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Offlinelostlogic
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Registered: 10/30/00
Posts: 34
Last seen: 14 years, 10 months
Re: help with contamination (pic)
    #283964 - 04/03/01 04:16 AM (15 years, 10 months ago)

Does everyone agree - these are the only fruiting shrooms and the contamination has not reached them, only half a cake left!!



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OfflineShroomzilla
enthusiast

Registered: 02/04/01
Posts: 177
Loc: Texas, home of the longho...
Last seen: 14 years, 11 months
Re: help with contamination (pic) [Re: lostlogic]
    #284593 - 04/03/01 04:46 AM (15 years, 10 months ago)

you can start over with a piece of clean tissue from inside one of the mushrooms, as long as they look nice and white inside they should be fine. If you do it quick before the mold gets to the inside of the cake, you can also start again with a core sample from the cake itself.

Spores or any "exposed" tissue probably already have some mold spores on them. Trichoderma is a very rampant spore producer and hard to kill. H2O2 will NOT kill it, the foaming action will just spread the spores around. It does hate basic conditions though, so mixing up a slurry of lime [mineral...not the fruit] and water will slow it down.

If 1/2 the cake is already green...time to abandon ship. Pick any shrooms then rinse [scrub] the cake under running tap water . This will reduce, but not eliminate, the spore count on the surface. Then take a knife and start carving out the green half till you're left with a clean nub. move to your "clean area" and sterilize your knife well, then "skin" the chunk. Sterilize the knife again and you can then chop that remaining bit up into smaller bits, maybe 4-5 mm square. This will be great spawn for starting off some new jars. You'll be amazed how fast your jars will colonize when started from established mycelia.



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OfflineNeonBlack
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Registered: 11/24/00
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Re: help with contamination (pic) [Re: lostlogic]
    #284661 - 04/03/01 09:53 AM (15 years, 10 months ago)

If I were you, I'd just let the shrooms grow and take some prints.. Any spores are better than no spores at all.. If it was me in your situation I'd probably eat the shrooms.. I don' t think they pose any risk at all. But, that's just me.



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Offlinetoadlicker
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Registered: 02/27/01
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Last seen: 15 years, 9 months
Re: help with contamination (pic) [Re: NeonBlack]
    #284684 - 04/03/01 10:28 AM (15 years, 10 months ago)

The problem with taking spores from these mushrooms is dealing with the green mold. As was already stated this stuff is nasty. That sucks that it took your only cake but you are going to batttle this mold every time if you take prints from these mushrooms. Either take that core sample or make mushy water and grow new jars from this.



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OfflineNeonBlack
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Re: help with contamination (pic) [Re: toadlicker]
    #284837 - 04/03/01 02:51 PM (15 years, 10 months ago)

Well that's a given.. I wouldn't bother with liquid culture quite yet though.. I'd grow the spores out on agar and make sure that any mycelium used is sterile.. If you put it into liquid media, you'd still have the problem with the mold. Go the agar route.



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Offlinelostlogic
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Registered: 10/30/00
Posts: 34
Last seen: 14 years, 10 months
Re: help with contamination (pic) [Re: NeonBlack]
    #286033 - 04/05/01 07:41 AM (15 years, 10 months ago)

Thanks for the advice

A further 8 shrooms have formed, but the contamination has reached all sides of the cake. The cake was thrown out.

I have decided to take 2 spore prints from 2 shrooms which looked healthy, which has worked well, not quite as big as the print from the vendor but quite thick.

I have also mixed some home made agar (Malt extract, peroxide and agar agar) and pored into 6 small jars. I have taken one small immature shroom and cut it into small chunks and added to 4 of the jars. The other 2 where inoculated with mycelia from a healthy part of the cake.

I hope to use agar colonised chunks to inoculate grain jars.

Further advise would be much appreciated.




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