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BlindMyce
Stranger

Registered: 06/02/23
Posts: 24
Last seen: 1 year, 2 months
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MS syringe>agar cleanup>grow VS syringe>grow>select primo fruit? 1
#28395879 - 07/14/23 11:10 PM (1 year, 6 months ago) |
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I’m finding MS syringes to be very hit or miss. Some have performed wonderfully straight from the vendor while some are nothing but a bunch of inactive spores in an E. coli soup (seriously, massive E. coli contamination in one that I received from a well established site sponsor).
Lots of people say you need to put it on agar, clean it up over the course of a few weeks via transfers, and then use the clean (and genetically much smaller) culture on the agar. But, at this point you don’t even know anything about how what you have left at the end of the process will actually grow & fruit. And I suppose you toss the syringe, minus the 10uL you pulled out on a loop in the beginning?
Conversely, I’ve seen some suggestions that you just grow right from the whatever you knock up with the MS (it either works or not depending on how badly it’s contaminated) then select the fruits that show the most desirable combination of characteristics and then go to agar with a print or tissue sample of this known desirable fruit genetics.
Like i said, I’ve seen both of these routes being advised but I haven’t seen a thread where they are compared and debated against one another. So let this be that place.
I kind of favor the logic of the second one, just knock up some grain with the genetic universe of the MS syringe and be able to select based on desired phenotype. Sure, some may not work at all or some might have a little bacteria that slows things down, but are the complete failures even worth the trouble? Some will certainly work and bear fruit. I just hate the idea of selecting and cleaning things up for weeks not knowing if what you’ll have in the end will actually be any good where it really matters. One of the great things about MS is the genetic diversity, there could be some monster genetic lineage in there but if you could be wasting your time with some deformed midget producer because you’re just worried about cleaning up the myc on agar first.
Would love to hear your thoughts on which route to go.
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Pickle Rick
Gherkin



Registered: 07/24/22
Posts: 866
Loc: Jar
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Re: MS syringe>agar cleanup>grow VS syringe>grow>select primo fruit? [Re: BlindMyce]
#28395940 - 07/15/23 02:13 AM (1 year, 6 months ago) |
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It boils down to contamination rates. In the second scenario the likelihood of growing anything useful is low. Not impossible, but low. Once you've cleaned up a culture, provided you've done everything else correctly - chances of contamination are fairly low.
-------------------- P i c k l e R i c k
Pick Rick's Mushroom Chocolate
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Hysteria
Bum Looker


Registered: 02/19/23
Posts: 996
Loc: New England USA
Last seen: 1 month, 27 days
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Re: MS syringe>agar cleanup>grow VS syringe>grow>select primo fruit? [Re: BlindMyce] 2
#28395951 - 07/15/23 02:48 AM (1 year, 6 months ago) |
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I think the consensus is on the 2nd method, but not spores to grain. Rather, spores to brf cakes (pf tek), where your chances of avoiding contamination are much greater. Personally, I did both concurrently, and I was able to succesfully get fruits from both the cleaned up cultures from mss to agar->grain->bulk substrate, and from pf tek, where I used those fruits for clones and prints for future grows. I'm not an expert but that has been my experience so far, and the results have been great. I feel like I have been able to make the best use of the original syringes that way. I didn't set out trying to reinvent the wheel. Just stood on the shoulders of the giants that built this place and followed their advice as best I could, and I can now say from experience that the teks on this site just work, as long as you put your head down and follow them.
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PBJ710
Strangler


Registered: 07/05/19
Posts: 3,301
Last seen: 6 hours, 42 minutes
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Re: MS syringe>agar cleanup>grow VS syringe>grow>select primo fruit? [Re: Hysteria]
#28395991 - 07/15/23 05:03 AM (1 year, 6 months ago) |
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IME when it comes to using spore syringes, PFTek is more likely to give you a diverse range of healthy fruits to clone/print from vs. spores > grain.
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iceNock


Registered: 11/15/21
Posts: 335
Last seen: 1 year, 4 months
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Re: MS syringe>agar cleanup>grow VS syringe>grow>select primo fruit? [Re: PBJ710]
#28396030 - 07/15/23 06:29 AM (1 year, 6 months ago) |
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Why does it have to be so extreme? Spores/Swab to agar, transfer what you know is clean growth and then run different sections of your T1 in a few days.
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RockinRobot
Stranger


Registered: 12/08/22
Posts: 1,893
Last seen: 6 hours, 11 minutes
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Re: MS syringe>agar cleanup>grow VS syringe>grow>select primo fruit? [Re: iceNock]
#28396051 - 07/15/23 07:01 AM (1 year, 6 months ago) |
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Agar is so simple. Even when I first started I had less than 2% contam rate on agar pours and transfers.
As others said if you want to shoot spores direct then use PF Tek then clone the fruit. The cloning still requires agar work though so may as well learn to do it with spores.
IMO if you can't work with Agar you are in the wrong hobby.
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