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InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28350162 - 06/06/23 09:18 PM (1 year, 7 months ago)

Quote:

MushyMom said:
Quote:

phenyl said:
Quote:

MushyMom said:
I’ve tested multiple ways to prep grain. I’ve been using purchased LC and agar I’ve prepped myself and no matter what I’m getting the green. The constant is being in my house. Next test will be sending to fruiting and storing the shoeboxes at a different location.  Just need to keep on keeping on - sometimes a failure is a success.




My bet is still on the SAB not being functionally effective in your environment. I had the same issue when I moved -- plagued me for about 9 months before I relented and bought an FFU. Problem immediately solved.

For me the giveaway was that most of my blank plates were forming colonies a few weeks after being poured. Beefing up my SAB technique helped but never quite solved it. Airbourne bacteria sucks.




Possible but I get 99% clean plates when I pour agar. My jars appear clean but then fail when sent to fruiting - this is across multiple batches done at different tones. If it was the SAB I would expect a higher contamination rate from my plates and a lower contamination rate from my boxes.

I’m not ruling out the SAB. I might try bringing the sab over and inoculating jars at my friends house when I go over to mix coir and make shoeboxes.

I wish I could get a ffu but I really don’t have the space for it right now. Until someone creates a portable one I’m kind of out of luck.




Even has handles

It's 2 feet wide. Smaller than my SAB.

Definitely an investment though.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Offlinephenyl
Stupid person
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Registered: 09/03/22
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Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom] * 1
    #28350363 - 06/07/23 03:01 AM (1 year, 7 months ago)

Quote:

MushyMom said:

Possible but I get 99% clean plates when I pour agar. My jars appear clean but then fail when sent to fruiting - this is across multiple batches done at different tones. If it was the SAB I would expect a higher contamination rate from my plates and a lower contamination rate from my boxes.





How long are you leaving your plates out for? I had 99% clean plates that would gradually fall to about 10% clean plates after a month of storage. Bacteria won't grow as quickly on agar as a 3d substrate that gets shaked up frequently.

- Bacteria and random molds (pink green and white!) are persistent problems.
- Multiple grain prep techniques have been used without effect; unlikely the issue.
- Multiple inoculant sources have been used without effect; unlikely the issue.
- Substrate prep is overwhelmingly unlikely to be an issue.

My suspicion therefore is that something from the environment is getting into your jars at inoculation.

It's possible you have (macroscopically) invisible bacteria or mold on your plates but with the variety of molds you've dealt with sporulating in glassware I'm inclined against suggesting it as a primary contaminant source. Unless, of course, your grain prep was at some point ineffective and the cause of those molds; I can't see if you've done control studies to see if your jars contaminate without inoculation.

What I am inclined to suggest is that your jars are almost always bacterially contaminated and killing off the mycelium, allowing molds to germinate after a few weeks. You can sometimes seemingly observe that happening if you leave a heavily bacterial jar for long enough.


Here's my favourite tub from when I was struggling with phantom contamination. The "pillows" turned orange after another week and the entire thing went green, lol. I was so pissed.


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The fool who persists in his folly will become wise.

Edited by phenyl (06/07/23 03:16 AM)

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Offlinejohnukguy
Learning
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Registered: 06/23/22
Posts: 2,423
Loc: Colorado Flag
Last seen: 1 year, 2 months
Re: Diagnosing my contamination issues/reviewing technique [Re: Way] * 1
    #28350364 - 06/07/23 03:01 AM (1 year, 7 months ago)




Dammit man, yet another longed for material thing that I have to put on my Amazon wish list. At this rate, I'll be starting an OnlyFans account soon.


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OfflineMushyMom
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Registered: 07/19/21
Posts: 246
Last seen: 1 year, 6 months
Re: Diagnosing my contamination issues/reviewing technique [Re: MushyMom]
    #28395685 - 07/14/23 06:19 PM (1 year, 6 months ago)

So life has been very busy and I haven’t had time to do any cult work.

Today I found out that there is a slow leak in the plumbing of the unit upstairs from mine that has been there for a while.  There is significant water damage and the M word growing in the walls.  They are going to need to tear apart my bathroom ceilings and walls and basically redo everything. Working with insurance and a restoration company to get this resolved but according to the plumber this could have been there for up to a year. 

So if I didn’t have a bunch of crap flying around my condo before - I do now. I’m guessing my cult work needs to go on hold for a bit longer :frown:


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