Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
OfflineDnice1
Young Grasshoppa
I'm a teapot
Registered: 01/13/23
Posts: 104
Last seen: 3 days, 8 hours
LC to make more LC. Clones to make LC - genetic drift?
    #28394916 - 07/14/23 01:28 AM (1 year, 6 months ago)

Alright fellas.

I’ve been trying to read up on. But I just feel like I’m confusing my self lol. Explain to this to me like I’m 12.

If I have a good liquid culture, can I squirt a tiny pit on a agar plate, and if that is CLEAN, just dump the plate into a jar of agar mix to make more liquid culture?

From what I have read. Yes. But no more than 3-4 times (transfers) - why? Is it genetic drift? Or the genetics change/weaken?

Let’s say after the 3rd crop, using the 3rd set of LC, I find a nice looking mushroom, I take a piece of the stipe, and put that on an agar plate….. does that mean I just started over to the “first transfer”? Or am I still on the 3rd?

Basically, I have a good genetic liquid culture that I’ve been running for a few runs now, and I thought I could just keep recycling the liquid culture forever. I’m now learning that is not the case and I’ll eventually run into a dud harvest.

Thanks for helping the noob fellas.

Edited by Dnice1 (07/14/23 01:29 AM)

Extras: Filter Print Post Top
InvisiblePickle Rick
Gherkin
I'm a teapot User Gallery


Registered: 07/24/22
Posts: 866
Loc: Jar
Re: LC to make more LC. Clones to make LC - genetic drift? [Re: Dnice1]
    #28394993 - 07/14/23 05:09 AM (1 year, 6 months ago)

If you have good LC that you've already fruited successfully you dont need to take it to agar to test.

To expand it, you can take it to agar to make more lc. Or you can aspire some of your current lc into a syringe and inoculate a new batch of lc. Agar is not used in classic lc other than for inoculation, most people use light malt extract (lme) or grain water but you can also use potato dextrose. Agar is used in semi solid lc (sslc) which apparently has better colonization time. Either way its not just a jar of agar to dump your plates/lc into.

If you're growing from a multispore lc, its worth finding a suitable candidate to clone immediately (largest fruit from your largest cluster is generally a good place to start) to stabilize and maximize your next grow.

Not sure about weakening. I have read about it but I'm not entirely sure when it happens. Unless I'm mistaken, I think this can be avoided by cloning a fruit; tissue sample -> agar -> new lc. That said, you can expand your lc quite a bit before having to do this.


--------------------
P i c k l e    R i c k :dancer:



Pick Rick's Mushroom Chocolate

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Far into the LC Myc Matrix
( 1 2 3 all )
agar 9,629 44 05/26/05 01:36 PM
by Aeolus1369
* clone question whitesox 960 5 02/21/03 08:27 AM
by z@z.com
* clone fruitbody on popcorn accesstwo 1,837 13 05/27/06 02:15 PM
by accesstwo
* Genetics daywalker 921 5 12/23/03 08:49 AM
by micro
* fruiting clone - will fruits resemble the master ? ohmatic 1,326 4 01/09/05 01:39 PM
by hyphae
* Liquid Culture help Kaydren 1,300 6 05/23/05 01:59 PM
by VALIS
* Various Questions -- LC, Tyvek and Continuous Myc splifner180 1,216 6 04/12/06 02:37 PM
by cappa
* LC Problems (It doesn't do a thing!) NESpores 860 7 01/28/06 05:49 PM
by blueferret

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
77 topic views. 18 members, 77 guests and 56 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.007 seconds on 12 queries.