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covertjoy

Registered: 07/09/23
Posts: 272
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Haploid isolates from spore syringes?
#28391739 - 07/10/23 08:00 PM (1 year, 6 months ago) |
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Is it possible to create haploid isolates on agar from purchased spore syringes? Or would the spores inside the syringe have already combined into diploids?
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: Haploid isolates from spore syringes? [Re: covertjoy]
#28391762 - 07/10/23 08:13 PM (1 year, 6 months ago) |
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Yep, it would be using serial dilution and a microscope
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covertjoy

Registered: 07/09/23
Posts: 272
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Re: Haploid isolates from spore syringes? [Re: SirPsycho]
#28391769 - 07/10/23 08:17 PM (1 year, 6 months ago) |
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Thanks. The microscope is to determine the CFUs and identify a culture as haploid right? Is there any way to do that just via observation?
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Edited by covertjoy (07/10/23 08:17 PM)
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
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Re: Haploid isolates from spore syringes? [Re: covertjoy]
#28391771 - 07/10/23 08:17 PM (1 year, 6 months ago) |
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Quote:
covertjoy said: Thanks. The microscope is to identify a culture as haploid right? Is there any way to do that just via observation?
No
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Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens
Balance in life is like running on ice.
π
π
π
£π
£π
π
π
π
π
π
   
"Mist your balls and fan your asshole" - Pandaskis, 2023
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covertjoy

Registered: 07/09/23
Posts: 272
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Re: Haploid isolates from spore syringes? [Re: SirPsycho]
#28391777 - 07/10/23 08:18 PM (1 year, 6 months ago) |
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Appreciated, thanks
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Eclipse3130
Servant of the Fungi



Registered: 10/06/13
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Re: Haploid isolates from spore syringes? [Re: covertjoy]
#28391857 - 07/10/23 09:16 PM (1 year, 6 months ago) |
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Quote:
covertjoy said: Is it possible to create haploid isolates on agar from purchased spore syringes? Or would the spores inside the syringe have already combined into diploids?
If you're trying to breed cubensis, it's very easy to do without isolating monokaryotic mycelium, you can just simply combine fresh spores together.
The breeding route isolating monos is inferior imo, as you aren't given the option to select for natural compatibility, vigor, health etc.
The natural breeding process accomplishes all of this and allows you to select for such
-------------------- "In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"
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covertjoy

Registered: 07/09/23
Posts: 272
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Re: Haploid isolates from spore syringes? [Re: Eclipse3130]
#28392110 - 07/11/23 06:02 AM (1 year, 6 months ago) |
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Quote:
Eclipse3130 said:
Quote:
covertjoy said: Is it possible to create haploid isolates on agar from purchased spore syringes? Or would the spores inside the syringe have already combined into diploids?
If you're trying to breed cubensis, it's very easy to do without isolating monokaryotic mycelium, you can just simply combine fresh spores together.
The breeding route isolating monos is inferior imo, as you aren't given the option to select for natural compatibility, vigor, health etc.
The natural breeding process accomplishes all of this and allows you to select for such
What would the specific process for that be? I.e. if breeding two strains, how would one determine which cultures are of both parents and not from two spores from a single parent?
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,721
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Re: Haploid isolates from spore syringes? [Re: covertjoy] 1
#28392153 - 07/11/23 07:05 AM (1 year, 6 months ago) |
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For spore crossing, the easiest way is to use two varieties that have very distinct stabilized traits. PE and any albino or Lucy fruit is a good match. PE doesnβt drop spores readily so if you get a pigmented fruit thatβs dropping spores, thereβs a decent chance itβs a crossed fruit. Then in the f2 gen, you should see the traits from both parents present in that gen when using high MS.
For example⦠this is a f2 of a melmak and yeti using heavy ms

If youβre trying to cross, say, GT and B+ by spore mixing, itβs gonna be extremely hard to determine if you actually got a cross because they both look so similar
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Eclipse3130
Servant of the Fungi



Registered: 10/06/13
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Loc: PNW
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Re: Haploid isolates from spore syringes? [Re: covertjoy]
#28393635 - 07/12/23 03:47 PM (1 year, 6 months ago) |
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Quote:
covertjoy said:
Quote:
Eclipse3130 said:
Quote:
covertjoy said: Is it possible to create haploid isolates on agar from purchased spore syringes? Or would the spores inside the syringe have already combined into diploids?
If you're trying to breed cubensis, it's very easy to do without isolating monokaryotic mycelium, you can just simply combine fresh spores together.
The breeding route isolating monos is inferior imo, as you aren't given the option to select for natural compatibility, vigor, health etc.
The natural breeding process accomplishes all of this and allows you to select for such
What would the specific process for that be? I.e. if breeding two strains, how would one determine which cultures are of both parents and not from two spores from a single parent?

A better question would by why would you cross two strains that you wouldn't be able to tell the difference of? Stuff like Golden teacher x Orissa India would be rather useless, both standard gold capped cubes.
Have 2 strains that are clearly unique from one another, breed them and they will show traits of each very clearly.
You can simply smash spores together, fresh works best as getting near identical germination time is key for them to breed.
-------------------- "In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"
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