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Rastuswatermelon
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Registered: 06/08/19
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Creating a warm dark place for inoculation/cultivation
#28391842 - 07/10/23 09:03 PM (1 year, 6 months ago) |
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Hi guys, serial poster I am, I know, but just trying to make sure I give my first grow the best chance possible.
I’m going to start the inoculation and cultivation process in the next few days (penis envy).
I understand it is good to let the grain bags etc go through that process in a dark warm place.
The only thing I can think of is in my wardrobe, but even then it’s only 18 degrees Celsius (when the heater is on at night, colder during the early morning during sleeping hours and during the day).
I was thinking of building and insulating a timber box for the process.
Or should I just do it in the mono tub and allow them to get a bit of light? (The only room I can do it in has a window and a skylight - so they will be on some form of daylight at all times).
Thanks in advance!
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johnukguy
Learning



Registered: 06/23/22
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Loc: Colorado
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Re: Creating a warm dark place for inoculation/cultivation [Re: Rastuswatermelon] 1
#28391864 - 07/10/23 09:24 PM (1 year, 6 months ago) |
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Doing anything in the dark with cubes is outdated. It's been found that a little light and air are beneficial from the get go. Also, just in case, put spores to agar, not to straight to grain, and then use clean mycelium from agar to grain.
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Baba Yaga
Psychedelic Minion

Registered: 09/13/20
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Loc: Try 'n' Error Boot Camp
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Re: Creating a warm dark place for inoculation/cultivation [Re: Rastuswatermelon] 1
#28391866 - 07/10/23 09:25 PM (1 year, 6 months ago) |
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Keeping inoculated spawn in the dark and at elevated temperatures is old information and can often cause more problems than doing any good especially if using a heat pad or something else that warms the bag unevenly. Keep you bag at room temperature on a shelf, that is all it needs and light or no light a this stage wont make mush of a difference. 18C will work, it only takes a bit longer. You could put something over your bags like a blanket but I rather try heat the room a bit more to 20C or 22C and leaven em uncovered so one doesn't have to fumble around to mush to have a look at the bag(s).
Definitely do not use any heat pads.
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Rastuswatermelon
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Re: Creating a warm dark place for inoculation/cultivation [Re: Baba Yaga]
#28392013 - 07/11/23 01:27 AM (1 year, 6 months ago) |
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Quote:
Baba Yaga said: Keeping inoculated spawn in the dark and at elevated temperatures is old information and can often cause more problems than doing any good especially if using a heat pad or something else that warms the bag unevenly. Keep you bag at room temperature on a shelf, that is all it needs and light or no light a this stage wont make mush of a difference. 18C will work, it only takes a bit longer. You could put something over your bags like a blanket but I rather try heat the room a bit more to 20C or 22C and leaven em uncovered so one doesn't have to fumble around to mush to have a look at the bag(s).
Definitely do not use any heat pads.
Thanks for the info guys! I will just keep it in the bedroom with natural light then. The room only gets to 18 degrees for a few hours at night when the ducted heating is on, rest of the time it’s about 14-16 degrees (early morning through to when we put the heater on at night.
I have an oil heater which I can use to bring the room to temp, but I presume it’s a bit of a safety hazard leaving it on 24/7 for the whole cultivation period. If that is the case, perhaps I just turn it in every now and then - surely that’s better than cold temps the majority of the time?
Other alternative is to not heat the room outside of the hours that we have the ducted heating on at night. I presume that will slow the process down dramatically. (Or will it halt the process all together?). At least then on a few months time when spring comes around the temps will pick up a bit (house still rarely gets above 20 degrees (from memory) even in warm weather - old double brick house. So artificial heating might be a necessity.
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Rastuswatermelon
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Re: Creating a warm dark place for inoculation/cultivation [Re: johnukguy]
#28392014 - 07/11/23 01:31 AM (1 year, 6 months ago) |
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Quote:
johnukguy said: Doing anything in the dark with cubes is outdated. It's been found that a little light and air are beneficial from the get go. Also, just in case, put spores to agar, not to straight to grain, and then use clean mycelium from agar to grain.
Thanks. I have live cultures in a syringe which I bought from an online vendor which has all been done in a sterile environment.
Do I still need to use agar?
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Ultravisit0r
apprentice



Registered: 04/03/23
Posts: 55
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Re: Creating a warm dark place for inoculation/cultivation [Re: Rastuswatermelon]
#28392016 - 07/11/23 01:33 AM (1 year, 6 months ago) |
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When you feel comfortable the temperature it's fine for cubes. 14-16 c° should work, maybe not the fastest. Patience gets you far tho
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Ultravisit0r
apprentice



Registered: 04/03/23
Posts: 55
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Re: Creating a warm dark place for inoculation/cultivation [Re: Rastuswatermelon] 1
#28392017 - 07/11/23 01:34 AM (1 year, 6 months ago) |
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SQuote:
Rastuswatermelon said:
Quote:
johnukguy said: Doing anything in the dark with cubes is outdated. It's been found that a little light and air are beneficial from the get go. Also, just in case, put spores to agar, not to straight to grain, and then use clean mycelium from agar to grain.
Thanks. I have live cultures in a syringe which I bought from an online vendor which has all been done in a sterile environment.
Do I still need to use agar?
Better to be safe than sorry. LC is easily contaminated during production. Best practice is to test it on agar
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Rastuswatermelon
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Re: Creating a warm dark place for inoculation/cultivation [Re: Ultravisit0r]
#28392024 - 07/11/23 02:00 AM (1 year, 6 months ago) |
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Ok no worries. I’ll be patient/will turn the heater on every now and then haha.
And yup I’ll look into to doing the agar too.
Thanks for the advice!
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johnukguy
Learning



Registered: 06/23/22
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Loc: Colorado
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Re: Creating a warm dark place for inoculation/cultivation [Re: Rastuswatermelon]
#28392025 - 07/11/23 02:07 AM (1 year, 6 months ago) |
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Agar is easy and cheap to make. Testing LC on it is quicker than doing spores to agar as well. You should know within a few days at the outside, if the LC is clean.
-------------------- How to post pictures to shroomery TEK
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“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
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Rastuswatermelon
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Re: Creating a warm dark place for inoculation/cultivation [Re: johnukguy] 1
#28392026 - 07/11/23 02:12 AM (1 year, 6 months ago) |
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Quote:
johnukguy said: Agar is easy and cheap to make. Testing LC on it is quicker than doing spores to agar as well. You should know within a few days at the outside, if the LC is clean.
Ok cool, that does sound wise. Would hate to wait for a longer time than normal due to the colder temps only to find out I have mould haha
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Yuggoth
Mi-Go Cultivator



Registered: 03/04/23
Posts: 558
Loc: Lost Carcosa
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Re: Creating a warm dark place for inoculation/cultivation [Re: Rastuswatermelon]
#28392311 - 07/11/23 10:25 AM (1 year, 6 months ago) |
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The last bags I did I put on a shelf at room temp, and they were fine, but I noticed that colonization was a little quicker on the bottom and on the sides that touched the wall, or the other bag--and those sides were also obviously warmer when touched. I ended up rotating the bags every week or so to account for this.
Maybe putting the bags in a simple carboard box would help them hold on to the heat they generate.
But also, you could just not worry about it.
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Rastuswatermelon
Stranger

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Re: Creating a warm dark place for inoculation/cultivation [Re: Yuggoth]
#28392520 - 07/11/23 02:15 PM (1 year, 6 months ago) |
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Quote:
Yuggoth said: The last bags I did I put on a shelf at room temp, and they were fine, but I noticed that colonization was a little quicker on the bottom and on the sides that touched the wall, or the other bag--and those sides were also obviously warmer when touched. I ended up rotating the bags every week or so to account for this.
Maybe putting the bags in a simple carboard box would help them hold on to the heat they generate.
But also, you could just not worry about it.
Interesting. I’ll keep that in mind
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