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Offlinejumalauta
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Panaeolus Cyanscens, teks for cloning from growbox and growing?
    #28351812 - 06/08/23 09:00 AM (1 year, 7 months ago)

Dear shroomboiz and mushiegirlz....
I'm inclined to try growing panaeolus cyanescens. Most of my regular spore sources are out of stock on panaelous. So I decided to order a panaoelus growbox. And will try to extract spores and clones from that. Now  the growbox is still being delivered. I was wondering, is this feasible. Is there something specific that i need to take in consideration. Or problems that i might run into. I was planning to take clones on petri dishes. Make clone liquid cultures, and take sporeprints. Is this all doable? And how should i get from dishes and syringes to the mushies? Some say tub is not the way to go for panaeolus. But some say many things. What would be the way to go then?
Thanks in advance


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OfflinePho
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28351833 - 06/08/23 09:23 AM (1 year, 7 months ago)

Let the tub fruit and then make multiple clones and take multiple spore prints. You want to do multiple agar plates so you don't lose everything to contamination.


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InvisibleBaba Yaga
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: Pho]
    #28351865 - 06/08/23 09:52 AM (1 year, 7 months ago)

I have no experience with grow boxes, do they come fully colonized? If that is the case then you could open it in a SAB and transfer some mycelium to a few agar dishes, maybe use normal and also water agar for this first step to better your success rate. Clean it up with a few transfers. If you want to clone fruits from the grow box then take clones to water agar first...really makes it easier.

Growing in tubs can work very well.

At the end of the day you can do a lot of things if your sterile technique is solid.

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Offlinejumalauta
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: Baba Yaga]
    #28351880 - 06/08/23 10:06 AM (1 year, 7 months ago)

Yes they come in a box that contains the fully colonised substrate.


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OfflineAspectOfTheCreator
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: Baba Yaga]
    #28351882 - 06/08/23 10:07 AM (1 year, 7 months ago)

Place clone tissue to water agar first.


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InvisibleBaba Yaga
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta] * 1
    #28351900 - 06/08/23 10:22 AM (1 year, 7 months ago)

Quote:

jumalauta said:
Yes they come in a box that contains the fully colonised substrate.





Then first thing I would do is to take a sample from the box to agar and clean it up. That way you hopefully will end up with a backup culture if the box is not going to produce anything.

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Offlinejumalauta
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28352840 - 06/09/23 03:18 AM (1 year, 7 months ago)

Thanks for great advice you all. I will keep you posted. I'm really excited about this. Most of all I can't wait to get some of them babies up in my top dome.:laugh: :laugh: :laugh:


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Offlinejumalauta
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28369993 - 06/22/23 11:06 AM (1 year, 6 months ago)

Update:









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InvisibleBaba Yaga
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28370198 - 06/22/23 01:30 PM (1 year, 6 months ago)

Nice!

Looks like you are getting all you wished for.

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Offlinejumalauta
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: Baba Yaga]
    #28370961 - 06/23/23 06:16 AM (1 year, 6 months ago)

Thank you Baba Yaga!

I have a question I see that one of the mushrooms has already sporulated. I am going to gather spores now from another and also clone one or two. The shrooms grow very uneven though. And there isnt a canvas of any sort? I see there are loads of small pins already waiting. Do I need to harvest them all at once? Or what should be done? I am almost ready to go for the second flush?


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InvisibleBaba Yaga
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28371083 - 06/23/23 08:33 AM (1 year, 6 months ago)

With a small substrate like this I would cut out the big fruits and leave the pins.

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Offlinejumalauta
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: Baba Yaga]
    #28374107 - 06/25/23 12:56 PM (1 year, 6 months ago)

Sorry to bother you Baba Yaga. But since you are in my eyes the resident Panaeolus-wiz, i'm gonna ask away. In your first post you suggested to first take clones from the mycelium from the growbox before fruiting the box. Now they are already fruiting and i took 10 clones from the fruits to water agar. I was wondering can I still use the boxed mycelium as clone source? There's some nice looking fluffy mycelium on top of the casing layer.


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InvisibleBaba Yaga
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28374224 - 06/25/23 03:15 PM (1 year, 6 months ago)

To be honest, if you have cloned a few fruits and been able to get some spores then I wouldn't bother. I thought to get a culture going would be a good idea since the kit was the only thing you had. But to answer your question, yes you can take a sample from the kit but it would have been easier to do it first thing assuming that the substrate colonized under sterile conditions. Now you might have some cleaning up to do.

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Offlinejumalauta
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28391428 - 07/10/23 03:13 PM (1 year, 6 months ago)

Yay everything turned out really nicely. Got some nice sporeprints. Cloes on water agar. And sampled the fruits on weekend. They are really really nice. The clones are filling the dishes slowly. Some of them have visible bacterial growth some of them have slowly advancing bacteria with mostly clean mycel. Some even seem to have no contams at all. So the next step would be to transfer them to nutrient agar right? I already prepared an oat flake juice agar.

so here's an obvious contam am I correct?


And here hopefully visible with mycelium and contam in center. I know it looks like there's contam in the upper part of the pic. But thats just light glare from fluorescent tubes.


So this is my first time cleaning agar. So I can observe that the slimy part in every of the contaminated starts in the piece that i cut from the shroom. So starting from the same point as mycelium. But advancing much slower. (both grow really slow, but that must be because of water agar) So I should just avoid the slime when doing the transfers?


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InvisibleBaba Yaga
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta]
    #28391575 - 07/10/23 05:58 PM (1 year, 6 months ago)

Yes you definitely want to avoid the slime which is bacteria. Let it grow out so you can comfortably take a small piece from the leading edge of the mycelium while staying well away from the contamination and transfer to a nutrient agar plate. After this it could be already clean but you might want to expand the clean mycelium to a few more plates to have some for inoculation and some to keep as backup.

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Offlinejumalauta
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Re: Panaeolus Cyanscens, teks for cloning from growbox and growing? [Re: jumalauta] * 1
    #28391622 - 07/10/23 06:46 PM (1 year, 6 months ago)

Thank you Baba Yaga!

Been reading your panaeolus thread a lot. Great stuff there.


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