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OfflineZactopher
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Registered: 10/31/22
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Trouble growing PC
    #28388687 - 07/07/23 06:28 PM (1 year, 6 months ago)

I’m at a loss and need some guidance.

I have been trying to grow Golden Teacher for some time now with no success. From spores, I inoculated 2 agar plates. All work is done in front of a laminar flow hood. One plate has contam, and the other did not appear to be contaminated. However, both plates have not grown out, but have stalled. I transferred the non-contaminated plate to another agar plate, and this one, too, stalled. From this new plate, I have then inoculated one grain bag, and two grain jars. All of them free for about a week, then stopped growing, and instead produced a liquid at the bottom.

I grow gourmets quite frequently and rarely have contamination issues. The grains I have used are from the same steralization batches from which I’ve used to grow gourmets successfully. Is this just some weak strain? Could the mycelium actually be contaminated, even though it looks clean? Is it just a coincidence that all 3 attempts at inoculation have failed? Should I just abandon this mycelium and buy new spores, or should I keep doing transfers?


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OfflinePBJ710
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Registered: 07/05/19
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Re: Trouble growing PC [Re: Zactopher]
    #28388972 - 07/08/23 04:14 AM (1 year, 6 months ago)

Sorry to be the bearer of bad news, but that spore print is very contaminated.  You're either going to need to do lots of cleanup work on the cultures or get a new print.

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OfflineSockadin
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Registered: 01/03/10
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Re: Trouble growing PC [Re: Zactopher]
    #28388991 - 07/08/23 05:34 AM (1 year, 6 months ago)

Grains look really wet In the jar. The plates don't look great either.

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