|
Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 1 hour
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78] 2
#28386100 - 07/05/23 03:40 PM (6 months, 21 days ago) |
|
|
Quote:
altford78 said:
Quote:
Tri-Polar said:

Are these the plates you made with the new Maltoferm ?
I still see some boogers but it's looking better.
No actually thats the CBW, this is the new plate with the maltoferm, it turned out real nice in my opinion (even though cbw and maltoferm are both briess brand lmao) it showed a lot less "boogerage"

And this was the end of my media bottle after pouring 20 plates without swirling in between

-------------------- Intro to Shroomery The Sexy TEKsβ’ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
Edited by Tri-Polar (07/05/23 03:41 PM)
|
nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 10 hours
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] 2
#28386521 - 07/05/23 11:24 PM (6 months, 20 days ago) |
|
|
Experiment, well, more of a test I did.
Shrooms growing in outbuilding. Swabbed some, others I printed but did something I've never done, did the old "raised wire cookie cooling tray so the gills don't touch the foil."
Swabbed 5 plates in front of flowhood, 6 days later 3 had contams, worst one shown here. Same day noced 5 plates in front of flowhood using cookie tray spores, zero contams. I'm going to do that from now on. I sprayed the cookie tray with ISO before using, then let dry in front of hood. Next time might use flame on it, just to be more careful.
Swab plate, 2 or 3 types of contam (plus some shroom mycelium):
---
Cookie tray print plates, no contam, plus good shroom mycelium): 


-------------------- -NEW? Start here.
Edited by nektar61 (07/06/23 02:34 PM)
|
Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 1 hour
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] 2
#28386529 - 07/05/23 11:34 PM (6 months, 20 days ago) |
|
|
I'd say thats some pretty stellar results!
 What if you just wrap the cookie rack in foil and bake it for a few minutes, unwrap in front of hood?
-------------------- Intro to Shroomery The Sexy TEKsβ’ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
|
nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 10 hours
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar]
#28386535 - 07/05/23 11:38 PM (6 months, 20 days ago) |
|
|
Quote:
Tri-Polar said: I'd say thats some pretty stellar results!
Thanks. I thought so too.
Quote:
Tri-Polar said: What if you just wrap the cookie rack in foil and bake it for a few minutes, unwrap in front of hood?
I think you're kidding. If you're not, you'd have baked mushrooms and probably no spore print. haha
Wouldn't help. Time is what drops spores. I've even tried to speed it up with a vibrator. Didn't work and Bod posted a long explanation of why that wouldn't help.
Though I usually don't do spore prints in a shroom room, is a little too warm, room temp is good. If the mushroom doesn't dry at all, they'll drop more spores. Mine look a little dried, but they were way open when I printed them. I should have printed 16 hours earlier. Still dropped a lot of spores.
(Vibrators are good for stirring spore syringes though, as are touching syringe to a blender or a power drill handle.)
-------------------- -NEW? Start here.
Edited by nektar61 (07/06/23 12:26 AM)
|
Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 1 hour
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] 2
#28386562 - 07/06/23 12:05 AM (6 months, 20 days ago) |
|
|
Oh no I was responding to this
Quote:
nektar61 said: I sprayed the cookie tray with ISO before using, then let dry in front of hood. Next time might use flame on it, just to be more careful.
-------------------- Intro to Shroomery The Sexy TEKsβ’ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
|
nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 10 hours
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar]
#28386572 - 07/06/23 12:15 AM (6 months, 20 days ago) |
|
|
Quote:
Tri-Polar said: Oh no I was responding to this
Quote:
nektar61 said: I sprayed the cookie tray with ISO before using, then let dry in front of hood. Next time might use flame on it, just to be more careful.
Obviously I meant fire without ISO. But yes, funny.
About every 6 months someone on here mentions using a flame inside an ISOed SAB and almost starting a huge fire.
I wonder if any ever really do the worst to the point where they are unable to post on here about it.
I go out of my way to tell noobs not to use flame in an ISOed SAB.
I mean, "it doesn't matter what I think of you, all that matters is clean spawn", but not doing permanent injury to yourself is actually a little more important than clean spawn.
-------------------- -NEW? Start here.
|
Tri-Polar
Paramecium Brain



Registered: 08/23/18
Posts: 374
Loc: purgatory
Last seen: 1 day, 1 hour
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
#28386581 - 07/06/23 12:27 AM (6 months, 20 days ago) |
|
|
I dont think im getting my point across im sorry lol
I mean, instead of spraying iso or using fire on the cookie rack, maybe you could wrap it in a layer of foil and sterilize it in your oven, and then take it to the flowhood to unwrap and cool down
 Sorry if I'm not being clear im stoned 
Edit:
Hmm efficacy or maybe efficiency of oven sterilization seems to be a little bit of a debated topic, I had just remembered Bod had it in his spore syringe tek as an option to sterilize the shot glass. Seems 375 for 30 minutes may be feasible/worth trying. If it can handle flame though I guess that may be the easy option
-------------------- Intro to Shroomery The Sexy TEKsβ’ IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH. hey fuck you dont look at me
Edited by Tri-Polar (07/06/23 12:38 AM)
|
buddzy
Mush wrangler



Registered: 02/16/04
Posts: 245
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar] 2
#28386587 - 07/06/23 12:39 AM (6 months, 20 days ago) |
|
|
Quote:
Tri-Polar said: I dont think im getting my point across im sorry lol
I mean, instead of spraying iso or using fire on the cookie rack, maybe you could wrap it in a layer of foil and sterilize it in your oven, and then take it to the flowhood to unwrap and cool down
 Sorry if I'm not being clear im stoned 
Heβs saying to dry heat sterilize the cookie tray then print instead of iso for the cookie tray but your test shows a big jump in successful clean prints Iβd be happy with those results.
|
nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,241
Loc: Cube Satellite
Last seen: 8 days, 10 hours
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar]
#28386606 - 07/06/23 12:57 AM (6 months, 20 days ago) |
|
|
Quote:
Tri-Polar said: I dont think im getting my point across im sorry lol
I mean, instead of spraying iso or using fire on the cookie rack, maybe you could wrap it in a layer of foil and sterilize it in your oven, and then take it to the flowhood to unwrap and cool down
 Sorry if I'm not being clear im stoned 
Edit:
Hmm efficacy or maybe efficiency of oven sterilization seems to be a little bit of a debated topic, I had just remembered Bod had it in his spore syringe tek as an option to sterilize the shot glass. Seems 375 for 30 minutes may be feasible/worth trying. If it can handle flame though I guess that may be the easy option 
I thought you were making a joke, like "wouldn't it be funny to spray the tray with ISO then flame it?" I had no idea you were being serious and talking about the oven. You didn't say "oven" until now.
I wouldn't bother sterilizing in an oven. Would take a while, 375 for 30 minutes would heat up the apartment (it's already hot where I live without that), and use more energy. And no need to sterilize foil, comes off the roll sterile.
How old was that Bod tek? He's one of the top growers here, but some of his really old teks are stuff I think he abandoned later. Plus he has an old tek that involves two chemicals that if mixed would produce toxic gas.
Little flame I use would take seconds for cookie cooling tray. I use this to flame sterilize scalpels, this is what I'll use next time for the cookie tray (same type thing bod recommends in his SAB tek): https://www.amazon.com/BonJour-Butane-Culinary-Br%C3%BBl%C3%A9e-Aluminum/dp/B000G9OYX4/
-------------------- -NEW? Start here.
Edited by nektar61 (07/06/23 02:51 PM)
|
JW123
Nooby Nooby Noob


Registered: 01/17/22
Posts: 1,305
Loc: Tn
Last seen: 22 minutes, 31 seconds
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] 2
#28386702 - 07/06/23 04:26 AM (6 months, 20 days ago) |
|
|
I am just going to leave this link here. I don't use it, because I just double print in a SAB and cannot be bothered with all this extra work. https://www.shroomery.org/forums/showflat.php/Number/24732724#24732724
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 15 hours, 3 minutes
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
#28386734 - 07/06/23 05:15 AM (6 months, 20 days ago) |
|
|
Quote:
Arctic_Fox said: Then why did PE destabilize already?
If you're seeing a PE destabilize it's cause of a lack of proper pheno selection for cloning and spore collection. All fruits have lots of different genetics in them almost seems like anything can happen cause it kinda can. What may appear as a destabilization could just be a different pheno and at any time starting some spores from it could even yield what appears to be a revert back to that original pheno.
The practice of cloning and printing the correct pheno each time must be a diligent task. If you have a PE that's not showing what you want try some spores from a different source perhaps.
|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 15 hours, 3 minutes
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69] 4
#28386738 - 07/06/23 05:19 AM (6 months, 20 days ago) |
|
|
|
Arctic_Fox
Mr. Furious


Registered: 03/13/23
Posts: 257
Loc: Ontario
Last seen: 3 months, 18 days
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
#28386800 - 07/06/23 07:09 AM (6 months, 20 days ago) |
|
|
Oh, I've never grown PE. I was referring to Pastywhyte's talk about PE destabilizing. You can search the forums for it.
--------------------
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] 3
#28386918 - 07/06/23 09:53 AM (6 months, 20 days ago) |
|
|
The idea that agar won't grow anything if it is sterilized more than a half hour is one of the more dumb things I've ever seen here.
People routinely sterilized pasty plates or agar in bottles along with spawn for 2 hours just fine.
If your plates are not growing its you or your inoculum to blame
|
aPurpleCray0n
His Dudeness



Registered: 02/13/23
Posts: 290
Loc: π¨π¦
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
#28386927 - 07/06/23 09:57 AM (6 months, 20 days ago) |
|
|
Couple plates I was ready to send to grain after they filled out, now I have an alarm in the back of my head telling me I ruined them taking them out of the bag and putting them on the black fabric unwrapped to take a pic.. classic me π€£

|
rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 15 hours, 3 minutes
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: bodhisatta] 1
#28386946 - 07/06/23 10:06 AM (6 months, 20 days ago) |
|
|
Quote:
bodhisatta said: The idea that agar won't grow anything if it is sterilized more than a half hour is one of the more dumb things I've ever seen here.
People routinely sterilized pasty plates or agar in bottles along with spawn for 2 hours just fine.
If your plates are not growing its you or your inoculum to blame
Haha Hi Bod!!
|
Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,152
Loc: Stables
Last seen: 16 minutes, 8 seconds
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69] 3
#28386967 - 07/06/23 10:29 AM (6 months, 20 days ago) |
|
|
Yeah, itβs that stinkin live mycelium that grows on some plates but not the ones I had in the PC for 90 minutes! That has to be it.
|
Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: bodhisatta]
#28386969 - 07/06/23 10:32 AM (6 months, 20 days ago) |
|
|
Quote:
bodhisatta said: The idea that agar won't grow anything if it is sterilized more than a half hour is one of the more dumb things I've ever seen here.
|
mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
#28386983 - 07/06/23 10:43 AM (6 months, 20 days ago) |
|
|
Quote:
Stipe-n Cap said:
Quote:
bodhisatta said: The idea that agar won't grow anything if it is sterilized more than a half hour is one of the more dumb things I've ever seen here.

|
Arctic_Fox
Mr. Furious



Registered: 03/13/23
Posts: 257
Loc: Ontario
Last seen: 3 months, 18 days
|
Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
#28386990 - 07/06/23 10:53 AM (6 months, 20 days ago) |
|
|
Quote:
Smellyhobbit said: Yeah, itβs that stinkin live mycelium that grows on some plates but not the ones I had in the PC for 90 minutes! That has to be it.
Did you get around to performing the test again?
--------------------
|
|