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OfflineMurkla
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Registered: 07/05/23
Posts: 2
Last seen: 1 year, 3 months
Stalled colonization and other questions. (please help a beginner!)
    #28386199 - 07/05/23 04:42 PM (1 year, 6 months ago)

hello! This is my first post here and I would greatly appreciate some help.


I have grown mushrooms before, but only from ready-to-grow boxes.
This time I wanted to try it out myself.

So my situation is as follows.
I got a glass jar with some kind of grain in it, as well as vermiculite.
The jar was inoculated and the mycelium had begun to grow and cover about 20-30% of it what I could see.
But after that it didn't make much progress for a week. I'f any at all.
I can see no signs of decontamination or anything bad with it. (Maybe just that it seems a little dry since tha grain at the top is completely loose and scrambles around In the jar.
I have tried shaking it, and opening it very briefly to get some gas to exchange.

My plan is to scoop out small pieces of mycelium and put them in new jars as to inoculate them with the already grown mycelium.
And then dump the contents of the jar into a bigger box(monotub).
In regards to the air-filters for the monotub, is"cut to size" HEPA filters a good choise for those?
I have this large sheet of filter material that is meant for filtrating your cooking stove fumes before it gets sucked into the air intake.

I have planned to fill the monotub with a mixture of brown rice, vermiculite and rice flour.


Now my questions are
:

  • 1: is my jar dried out and that's why the mycelium isn't making any noticable progress?
    If so, what can I do about it to make it continue colonizing?
    If so, can I add more water to it?

  • 2: is it possible to get more fresh jars to inoculate with only some pieces of already cultivated substrate from the original jar?

  • 3: is my substrate recipe good for filling the monotub with after I have sterilized it and made it ready for dumping in the colonized contents of the jar?
    And should I have the substrate dry or wet?
    If it should be wet:
    Is it enough to boil that water for a while, let it cool and then add it to the substrate, or will that not get rid of any contaminations?


  • 4: when I sterilize the substrate for the monotub and future jars, can I do it in thicker plastic bags, or will they just melt inside the PC?

  • 5: should I add any water to the substrate before or after I sterilize it in my PC?

    I don't want to lose the mycelium I have right now because I don't have access to more spore-syringes to start over with at the moment.


    I would greatly appreciate your help in this.


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Offlinejohnukguy
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Registered: 06/23/22
Posts: 2,423
Loc: Colorado Flag
Last seen: 1 year, 2 months
Re: Stalled colonization and other questions. (please help a beginner!) [Re: Murkla] * 1
    #28386205 - 07/05/23 04:45 PM (1 year, 6 months ago)

Welcome Please post pics so that people can better advise you. Essentially however, not much of what you plan to do sounds like a good option to be honest.
If you do want to save any of the mycelium in a form that's usable, then learn to make some cheap, quick, agar and put a tiny bit to that (literally a speck will do).
Then transfer away from contaminants a few times until you have clean mycelium on agar to put to grain. Have you read any of the up to date Teks here yet?


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OfflineMurkla
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Registered: 07/05/23
Posts: 2
Last seen: 1 year, 3 months
Re: Stalled colonization and other questions. (please help a beginner!) [Re: johnukguy]
    #28386370 - 07/05/23 08:12 PM (1 year, 6 months ago)

I don't see any contaminants at all atm, it's just growing very slow or has stalled, and I wonder if I can do anything about speeding the colonization up, like maybe add some water to the jar that seems to have stalled in colonizing further after 30%.
Or if I can take some of the healthy mycelium and make new jars with that (letting it colonize new fresh jars.

The other questions are about if my materials would work fine as substrate to make a monotub and use the mycelium I have. Or if I really need some other things like Coco coir or something to bull up the final substrate.
I have brown rice, rice flour, vermaculite and perlite at the moment to use.

I'm really just looking for some tips of what is possible with what I have. (I can get some Coco coir but have a hard time getting new spores right now, so I would like to at least try to make something out of what I have going right now.

Thanks :smile:

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