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Invisibletrichoserious
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Registered: 07/05/23
Posts: 5
Been a long time
    #28385935 - 07/05/23 12:48 PM (1 year, 6 months ago)

I have not done anything like this in a long time(20 yrs or so), so I'm a bit rusty... Also still can't figure out how to change profile picture.

Anyway, could use a little help. Knocked up 10 days ago. This looks ok I guess. Like I said, been a while.

Any help or advice is greatly appreciated!


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OfflineBajazly
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Registered: 09/02/22
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Re: Been a long time [Re: trichoserious] * 1
    #28385941 - 07/05/23 12:59 PM (1 year, 6 months ago)

Welcome back.

If this is spores to grain you may be in for a long hard ride. Better to start off with PF Tek or learn agar. You will be much happier in the long run.

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Invisibletrichoserious
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Re: Been a long time [Re: Bajazly]
    #28385944 - 07/05/23 01:02 PM (1 year, 6 months ago)

This was liquid culture. Yeah it may take some time, I'm just concerned that it looks ok and not contam. Like I said, been a while lol

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Invisibletrichoserious
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Re: Been a long time [Re: trichoserious]
    #28385946 - 07/05/23 01:07 PM (1 year, 6 months ago)

I should also interject that space is an issue for me. That why I'm going this route with trying it all in the bag. Not ideal, but what I have to work with atm

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Offlinetholos
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Re: Been a long time [Re: trichoserious] * 1
    #28385948 - 07/05/23 01:10 PM (1 year, 6 months ago)

Quote:

trichoserious said:
This was liquid culture. Yeah it may take some time, I'm just concerned that it looks ok and not contam. Like I said, been a while lol





This was his point -- the best thing to do is to use an agar plate to check if your LC was contaminated or not

You will be able to tell much more quickly whether or not your syringe is contaminated on a 2 dimensional surface of an agar plate, compared to a three dimensional bag of grain. With the agar plate in 48-72 hrs you will know if your syringe is harboring any nasties and not just cubensis mycelium. On a grain bag, that could take days or weeks dependent on how sterile your grains are and how sterile your syringe is

If it is not contaminated, awesome, you have a clean syringe and now an agar plate that you can also use to inoculate and/or make more plates to knock up more jars

If it is contaminated, now you've only used one agar plate worth of materials to see that your culture will need to be cleaned up instead of being down an entire bag of grain/coir

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Invisibletrichoserious
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Re: Been a long time [Re: tholos]
    #28385951 - 07/05/23 01:13 PM (1 year, 6 months ago)

Ahhhh 👍

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OfflinePandaskis
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Re: Been a long time [Re: trichoserious] * 2
    #28385985 - 07/05/23 01:47 PM (1 year, 6 months ago)

Quote:

trichoserious said:
Ahhhh 👍




Tbh already looks a bit bacterial to me

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Invisibletrichoserious
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Re: Been a long time [Re: Pandaskis]
    #28386003 - 07/05/23 01:58 PM (1 year, 6 months ago)

Quote:

Pandaskis said:
Quote:

trichoserious said:
Ahhhh 👍




Tbh already looks a bit bacterial to me





I thought so as well. Why I asked.

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OfflinePandaskis
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Re: Been a long time [Re: trichoserious]
    #28386012 - 07/05/23 02:07 PM (1 year, 6 months ago)

Quote:

trichoserious said:
Quote:

Pandaskis said:
Quote:

trichoserious said:
Ahhhh 👍




Tbh already looks a bit bacterial to me





I thought so as well. Why I asked.




The wet spots on the already colonized grain mostly. Check out the clean spawn checklist in my siggy

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