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OfflineRLmushrooms420
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Registered: 07/03/23
Posts: 17
Last seen: 1 year, 2 months
Re: Is my liquid culture doing ok [Re: Pandaskis]
    #28384724 - 07/04/23 11:15 AM (1 year, 6 months ago)

That’s what mine looks like but it’s brown Onley been 4 days

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InvisibleStipe-n CapMDiscord
The Pride of Cucamonga
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Registered: 08/04/12
Posts: 8,269
Loc: Canada
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Re: Is my liquid culture doing ok [Re: RLmushrooms420] * 2
    #28384727 - 07/04/23 11:18 AM (1 year, 6 months ago)

Yep, I pour its straight from the media bottle directly into jars or bags. As seen here, disregard the amount being poured, I needed to use it all up :lol:



Notice how I keep the foil caps on the lids that are behind the jars receiving first air? This maintains sterility while in turbulent flow.

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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: Is my liquid culture doing ok [Re: Mycolorado]
    #28384729 - 07/04/23 11:22 AM (1 year, 6 months ago)

Quote:

Mycolorado said:
I’ve never had an issue with vacuum on a sterilized syringe.  If it’s an issue, order some sterile, individually sealed pipettes.  You could also use a loop.  And to be honest, I don’t test my LC.




Oh look, I'm just being a retard again. Just use a pipette tip or inoc loop. Right.

But what if the loop was not properly sterilized at the factory? Or what if I contam it while taking it out of the package?

LC is not for paranoid bitches lol.

I don't test my LC either. :fuckthepolice:

But I also grow mold as my day job so YMMV.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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OfflinePandaskis
Eating Bamboo
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Registered: 03/14/23
Posts: 1,869
Last seen: 8 months, 8 days
Re: Is my liquid culture doing ok [Re: altford78]
    #28384730 - 07/04/23 11:24 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
Yep, I pour its straight from the media bottle directly into jars or bags. As seen here, disregard the amount being poured, I needed to use it all up :lol:



Notice how I keep the foil caps on the lids that are behind the jars receiving first air? This maintains sterility while in turbulent flow.




:takingnotes: ill keep this all in mind when i graduate to LC and a Laminar flow hood in the distant future ;___;

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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: Is my liquid culture doing ok [Re: Stipe-n Cap]
    #28384740 - 07/04/23 11:35 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
LC is single use media, imo, so testing is an unnecessary risk/vector for contamination.






:emotionalmoment:

Fucking finally, a TC confirms I'm not insane. You can't test LC without having an  observer effect. It's not as bad as quantum entangled photons, but still you are fucking up the thing you are trying to test.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
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Re: Is my liquid culture doing ok [Re: Pandaskis] * 2
    #28384743 - 07/04/23 11:37 AM (1 year, 6 months ago)

Yes, free pour, too. 

At altford - make your own loop.

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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: Is my liquid culture doing ok [Re: Mycolorado]
    #28384750 - 07/04/23 11:43 AM (1 year, 6 months ago)

Just shove that bitch in there red hot? Sure why not. I have some spent LC, I've been thinking putting that shit to agar to see if it was contamed to begin with or if my unmodified jars were the vector. Will do.:boobsmell:


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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