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OfflineRLmushrooms420
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Is my liquid culture doing ok
    #28383417 - 07/03/23 09:54 AM (1 year, 6 months ago)

I am new to the hobby and wondering if my liquid culture is doing ok

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InvisibleStipe-n CapMDiscord
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Re: Is my liquid culture doing ok [Re: RLmushrooms420] * 1
    #28383451 - 07/03/23 10:23 AM (1 year, 6 months ago)

If you're new then my guess would be no. Those large surface bubbles aren't a good sign. I'd recommend not using LC at all until you've learned the basics.

The rule of thumb should be: if you have to ask if it's ok, it probably isn't.

If you insist upon using LC, this is how I do it:

https://www.shroomery.org/forums/showflat.php/Number/28104670

Give that a read.

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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: RLmushrooms420]
    #28383454 - 07/03/23 10:28 AM (1 year, 6 months ago)

It’s my second attempt but what what I was worried about it the light brown swirls the bubbles were from swerling it around it looks still unles I agitate it

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OfflineSirPsycho
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Re: Is my liquid culture doing ok [Re: RLmushrooms420] * 1
    #28383455 - 07/03/23 10:28 AM (1 year, 6 months ago)

Test it on agar.


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InvisibleStipe-n CapMDiscord
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Re: Is my liquid culture doing ok [Re: RLmushrooms420]
    #28383457 - 07/03/23 10:30 AM (1 year, 6 months ago)

The bubles are often an indication of microbial activity in the broth, with the exception of stirring/agitating.

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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: Stipe-n Cap]
    #28383497 - 07/03/23 10:53 AM (1 year, 6 months ago)

Ya pictures wore taken after like right after steering.

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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: RLmushrooms420]
    #28384595 - 07/04/23 09:29 AM (1 year, 6 months ago)

I’m not worried about the bubble like I said pic was taken right after agitating the jar but I have brown streaks it’s penis envy if that helps

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InvisibleStipe-n CapMDiscord
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Re: Is my liquid culture doing ok [Re: RLmushrooms420] * 1
    #28384597 - 07/04/23 09:30 AM (1 year, 6 months ago)

Test it on agar, as mentioned above.

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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: Stipe-n Cap]
    #28384609 - 07/04/23 09:48 AM (1 year, 6 months ago)

I don’t have any agar was considering just buying more syringes and using that to inoculate my grain spawn every liquid culture I do I end up with brown cetament on the bottom I inoculated another one sat looks better butt I’m hoping no cetement happens idk what I’m doing wrong?

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OfflinePandaskis
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Re: Is my liquid culture doing ok [Re: RLmushrooms420]
    #28384612 - 07/04/23 09:51 AM (1 year, 6 months ago)

What youre doing wrong is that youre attempting to do LC without first mastering Agar. All LC should be tested on Agar, LC is not a 2D Medium that you can see contamination in.

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InvisibleMycolorado
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Re: Is my liquid culture doing ok [Re: Stipe-n Cap]
    #28384614 - 07/04/23 09:52 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
If you're new then my guess would be no.  If you insist upon using LC, this is how I do it:

https://www.shroomery.org/forums/showflat.php/Number/28104670

Give that a read.



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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: Mycolorado]
    #28384616 - 07/04/23 09:58 AM (1 year, 6 months ago)

I’m just wondering what the brown streaks are they sit on the bottom until u agitate. I will order agar but should I just inculcate my spawn bags with syringes . Thanks for all the input

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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: RLmushrooms420]
    #28384617 - 07/04/23 10:00 AM (1 year, 6 months ago)

I have read a bunch of grow threads and liquid culture threads I shoulda boaght agar last time I ordered grow supplies

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InvisibleMycolorado
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Re: Is my liquid culture doing ok [Re: RLmushrooms420] * 1
    #28384626 - 07/04/23 10:09 AM (1 year, 6 months ago)

Spore syringes to LC is frowned upon due to the likelihood of contamination.  You haven’t posted the LC recipe but the sediment is likely ingredients but could be something funky…your pic leaves a lot to the imagination as it’s not very illustrative of what you’re referring to.  Spore syringes to grain are also often a gamble for the same reasons.  If you’re really wanting to grow with grain, start agar work and inoculate your grain and LCs with clean cultures. It can be done cheaply and is not difficult.  Otherwise, you should look into pf tek.  Ziran has a nice write up.

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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: Mycolorado]
    #28384634 - 07/04/23 10:16 AM (1 year, 6 months ago)

The liquid culture was boaght from midwest I already boaght all my substrate and spawn bags from them too

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InvisibleStipe-n CapMDiscord
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Re: Is my liquid culture doing ok [Re: RLmushrooms420]
    #28384635 - 07/04/23 10:17 AM (1 year, 6 months ago)

Quote:

RLmushrooms420 said:
The liquid culture was boaght from midwest I already boaght all my substrate and spawn bags from them too




Welp, that's going to produce a multitude of problems.

Here:

https://www.shroomery.org/forums/showflat.php/Number/28106983

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InvisibleMycolorado
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Re: Is my liquid culture doing ok [Re: RLmushrooms420]
    #28384639 - 07/04/23 10:20 AM (1 year, 6 months ago)

I don’t trust anything I didn’t make myself.  You’re also paying a premium on premade products and will save tons prepping yourself.  Homemade agar media, spawn and LC costs Pennies.

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OfflineRLmushrooms420
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Re: Is my liquid culture doing ok [Re: Stipe-n Cap]
    #28384642 - 07/04/23 10:22 AM (1 year, 6 months ago)

I get it it’s a science not a hobby

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InvisibleStipe-n CapMDiscord
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Re: Is my liquid culture doing ok [Re: RLmushrooms420] * 1
    #28384646 - 07/04/23 10:24 AM (1 year, 6 months ago)

It is a hobby that is founded on the science of microbiology, but don't let that dissuade you. It can be as easy or as complex as you'd like it to be.

By choosing to use LC as a new grower, without agar, you're definitely choosing the hard path.

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InvisibleMycolorado
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Re: Is my liquid culture doing ok [Re: RLmushrooms420] * 2
    #28384648 - 07/04/23 10:24 AM (1 year, 6 months ago)

Science, art, hobby. Sciartobby. :shrug:

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