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OfflineFrostyShroomer
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First agar transfer. DOA?
    #28380698 - 06/30/23 08:49 PM (1 year, 6 months ago)



First transfer from agar plate to agar plate. Came from a vendor in a sterilized bag. Transferred in SAB after spraying everything down with 70% iso. Plates for sure looked clear when I pulled them from package, mycelium is growing great but now I see these red specks all over my plate underneath the agar. Not really visible from the top. Were these plates contaminated from the get go or is it possible somehow I did something that caused this?

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OfflineCocaineBuffet
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Re: First agar transfer. DOA? [Re: FrostyShroomer]
    #28380724 - 06/30/23 09:08 PM (1 year, 6 months ago)

Its hard to tell from those pictures but the plates in the second picture seem to be good. In regard to the first picture there are some solid spots to transfer from if there is something I can't see. Kind of the point of agar is to keep sectoring healthy mycelium until that is the only thing left.

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OfflineFrostyShroomer
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Re: First agar transfer. DOA? [Re: CocaineBuffet]
    #28380753 - 06/30/23 09:39 PM (1 year, 6 months ago)



Here’s a top view, it sort of looks like I have primordia already, if that’s what that is? Doesn’t that typically happen if the mycelium can tell there’s some kind of contam?

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InvisibleYuggoth
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Re: First agar transfer. DOA? [Re: FrostyShroomer]
    #28380775 - 06/30/23 09:57 PM (1 year, 6 months ago)

The specs in your first picture look to me like browned bits of dry malt extract. Is that stuff on top or inside of the agar?


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We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo

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Offlinesmalltalk_canceled
Babnik
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Re: First agar transfer. DOA? [Re: FrostyShroomer]
    #28380787 - 06/30/23 10:06 PM (1 year, 6 months ago)

lookks good. do another transfer


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Willpower is the one true virtue


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OfflineFrostyShroomer
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Re: First agar transfer. DOA? [Re: Yuggoth]
    #28381093 - 07/01/23 07:31 AM (1 year, 6 months ago)

It’s actually inside the agar. I was hoping that’s all it was was bits of agar. It’s just they weren’t there when I first got them

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Babnik
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Re: First agar transfer. DOA? [Re: FrostyShroomer]
    #28381097 - 07/01/23 07:39 AM (1 year, 6 months ago)

ived seen commercial shipments send out plates like those, its a minor mistake leading to a if you ask me non important beauty loss


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Willpower is the one true virtue


Edited by smalltalk_canceled (07/01/23 07:40 AM)

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OfflineFrostyShroomer
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Re: First agar transfer. DOA? [Re: smalltalk_canceled]
    #28381287 - 07/01/23 11:05 AM (1 year, 6 months ago)

So you think I’m good to leave them alone and let them grow?

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