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OfflineGlockta
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Registered: 04/28/23
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Help identifying my pf tek
    #28378397 - 06/28/23 10:45 PM (1 year, 6 months ago)

Hey all,
Newbie here gone from spores/agar/liquid culture/pf Tek
These are meant to be GT, they have been in my fruiting chamber for 1 week





They dont appear to look like anything I have seen online, are they GT?

Regards

Edited by Glockta (06/28/23 11:06 PM)

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OfflineAnglerfishM
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Re: Help identifying my pf tek (moved) [Re: Glockta] * 1
    #28378523 - 06/29/23 02:32 AM (1 year, 6 months ago)

This thread was moved from Mushroom Hunting and Identification.

Reason:
Cultivation questions are better answered in cultivation forum.

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OfflinePBJ710
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Re: Help identifying my pf tek (moved) [Re: Anglerfish]
    #28378552 - 06/29/23 03:54 AM (1 year, 6 months ago)

It's one of those things that can happen when you don't test your cultures out before expanding them.  You would have likely seen better results by using MS > PFtek to obtain clone material and then clean it up on agar & expand it.

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OfflineGlockta
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Re: Help identifying my pf tek (moved) [Re: PBJ710]
    #28378559 - 06/29/23 04:03 AM (1 year, 6 months ago)

Sry I don’t understand, is my liquid culture that I made from spores bad? Will these of cakes fruit or is this it?

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Offlinephlanx
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Re: Help identifying my pf tek (moved) [Re: Glockta]
    #28378566 - 06/29/23 04:22 AM (1 year, 6 months ago)

Hey Glockta,

Welcome to the shroomery!

So, you made an LC using a spore syringe?  Thats a no no. There is no way to tell if you are transferring contams from a syringe to an LC without being able to test it on agar.

So, syringes are generally best used for PFTek or to agar for clean up.  They are inherently dirty because of the way mushrooms are grown and how spores are collected.  Fruiting is done (most often) in open air, so their spores are not clean right off the bat.

Okay, so you took that LC to PFtek jars, they seem to have colonized okay, which is great.  Is that tan material fruit material? Or is it mold?  Never seen anything like that.  It could just be really strange mutation, but I can’t see that being the case on all your cakes at once.

Were your spores from a trusted vendor?  Could they be a different species?

Doesn’t look like GT fruit to me.

Phlanx

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OfflinePBJ710
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Re: Help identifying my pf tek (moved) [Re: phlanx]
    #28378579 - 06/29/23 04:41 AM (1 year, 6 months ago)

The LC isn't 'bad' from a contamination point of view, but it does appear that the culture doesn't perform very well and produces small oddball shaped fruits.  IMO you need to test the fruits to see if the potency/aesthetics makes up for the low yields and if not, toss the culture and start a new project.

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Invisibledna24
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Re: Help identifying my pf tek (moved) [Re: Glockta]
    #28378758 - 06/29/23 08:24 AM (1 year, 6 months ago)

Quote:

Glockta said:
Sry I don’t understand, is my liquid culture that I made from spores bad? Will these of cakes fruit or is this it?



you did not make a liquid culture from spores, thats not how that works


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Invisiblemushhead
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Registered: 08/22/14
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Re: Help identifying my pf tek (moved) [Re: dna24] * 1
    #28378770 - 06/29/23 08:32 AM (1 year, 6 months ago)

We're getting a lot of these posts recently Randal.
I wonder which YouTuber is saying it's a good idea.
:frankensmoke:

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OfflinePandaskis
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Re: Help identifying my pf tek (moved) [Re: mushhead]
    #28378788 - 06/29/23 08:53 AM (1 year, 6 months ago)

:tubgirl:

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Invisiblestarvinghooker
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Re: Help identifying my pf tek (moved) [Re: Pandaskis]
    #28378884 - 06/29/23 11:09 AM (1 year, 6 months ago)

Didn't op say he made LC from agar? I'm confused, too


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OfflineGlockta
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Re: Help identifying my pf tek (moved) [Re: starvinghooker]
    #28378927 - 06/29/23 11:57 AM (1 year, 6 months ago)

Apologies I made it from Agar, then tested it again on agar to check for contamination, Thankyou everyone for your responses, much appreciated.

Edited by Glockta (06/29/23 12:00 PM)

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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,242
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Re: Help identifying my pf tek (moved) [Re: Glockta]
    #28378964 - 06/29/23 12:32 PM (1 year, 6 months ago)

Well you're on the right track!
Keep at the agar work, come back to LC when you have a cleaner plate.


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