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OfflineSporas
Mycelied one

Registered: 05/22/22
Posts: 30
Last seen: 8 months, 22 days
Worms on my agar plate and other questions on Agaricus sp. cultivation
    #28378491 - 06/29/23 01:18 AM (1 year, 6 months ago)

Hi, I was trying to clone an Agaricus sp., not yet identified.
I got successful petri dishes and grain spawn, but not yet a frutification.
Last ting first: one of my plate (grain transfer) went from full colonization to worms, little tiny white worms, dozens. The mycelum disappeared.
I 've noticed a similar phenomenon on some grain spawn: fully mycelized to nothing (but without worms and a very bad acid smell almost pee-like) in less than 24 hrs.
Here are some pictures, actually you can notice that the grain spawn (wild bird seeds) is not fully "mycelized", it seems it has a preference for small grains and some of them have been discarded. (See photos)

I suppose the worms were in the grain I used, probably one of the non-mycelized. But what about the disappeared mycelium?
Anyone has similar experience?




Thank you

Edited by Sporas (06/29/23 01:19 AM)

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Offlinephlanx
Experimentor


Registered: 06/11/05
Posts: 191
Loc: U.S.
Last seen: 4 months, 24 days
Re: Worms on my agar plate and other questions on Agaricus sp. cultivation [Re: Sporas] * 1
    #28378561 - 06/29/23 04:09 AM (1 year, 6 months ago)

Hey Sporas,

Sorry to read about your trouble.

Okay, so grain should be sterilized before we add any kind of culture to it, so anything in there should have been dealt with before inoculation.  Unless maybe you did an agar transfer and the contam rode in on bad agar?  How was your grain inoculated?

Little maggots like that are (i think) usually from flies, do you have fungus gnats? 

If your grain spawn stinks that badly, I would trash it, no question about that from my end.  Any plates that have maggots, unfortunately I would also trash those. I wouldn’t risk hatching flies directly in my grow space. 

If you are trying to clone from a wild specimen, then it seems you brought some bugs in with it. You could try to find some clean myc and very diligently transfer away from contams over a few plates, really the only option except to start over entirely.

Good luck!

Phlanx

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OfflineSporas
Mycelied one

Registered: 05/22/22
Posts: 30
Last seen: 8 months, 22 days
Re: Worms on my agar plate and other questions on Agaricus sp. cultivation [Re: phlanx]
    #28378585 - 06/29/23 04:56 AM (1 year, 6 months ago)

Ehi, thanks for your reply.
The grain was inoculated with agar. So probably the flies where on agar, right?

Ok, and what about the selection on grains the mycelium makes?
I saw other mycelum completely eat all grains of the same sort, this one seems has a more delicate taste. Any suggestion?
thank you

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OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
Last seen: 16 hours, 15 minutes
Trusted Cultivator
Re: Worms on my agar plate and other questions on Agaricus sp. cultivation [Re: Sporas]
    #28378587 - 06/29/23 04:58 AM (1 year, 6 months ago)

Quote:

Sporas said:
Ehi, thanks for your reply.
The grain was inoculated with agar. So probably the flies where on agar, right?

Ok, and what about the selection on grains the mycelium makes?
I saw other mycelum completely eat all grains of the same sort, this one seems has a more delicate taste. Any suggestion?
thank you



It's not covering the grain cause there's contaminants in there.

How did you make your grain?


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineSporas
Mycelied one

Registered: 05/22/22
Posts: 30
Last seen: 8 months, 22 days
Re: Worms on my agar plate and other questions on Agaricus sp. cultivation [Re: SirPsycho]
    #28379792 - 06/30/23 05:55 AM (1 year, 6 months ago)

Quote:

SirPsycho said:
It's not covering the grain cause there's contaminants in there.

How did you make your grain?




I pressure cooked them at 15psi for an hour. The fact is that all 10 jars with the same mycelium present the same pattern. It seems to me a behaviour of the mycelium on certain type of grains. Just my opinion.
Other jars pressure cooked  as well, with a different mycelium, were fully mycelized.

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OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
Last seen: 16 hours, 15 minutes
Trusted Cultivator
Re: Worms on my agar plate and other questions on Agaricus sp. cultivation [Re: Sporas] * 1
    #28379797 - 06/30/23 05:58 AM (1 year, 6 months ago)

Quote:

Sporas said:
Quote:

SirPsycho said:
It's not covering the grain cause there's contaminants in there.

How did you make your grain?




I pressure cooked them at 15psi for an hour. The fact is that all 10 jars with the same mycelium present the same pattern. It seems to me a behaviour of the mycelium on certain type of grains. Just my opinion.
Other jars pressure cooked  as well, with a different mycelium, were fully mycelized.



Those weren't sterilized, 90mins at least. And that's after letting steam vent for a minimum of 10 mins


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: Worms on my agar plate and other questions on Agaricus sp. cultivation [Re: SirPsycho]
    #28379813 - 06/30/23 06:11 AM (1 year, 6 months ago)

I assume you took a wild Agaricus so the most logical explanation is that the eggs were on the initial mushroom tissue. If not that, then you are using unmodified jars or the filters failed and let an insect lay eggs in the jars post inoculation. It doesn't make any sense for the larvae to be in the plate but not in the jar that the grain came from.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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OfflineSporas
Mycelied one

Registered: 05/22/22
Posts: 30
Last seen: 8 months, 22 days
Re: Worms on my agar plate and other questions on Agaricus sp. cultivation [Re: SirPsycho]
    #28380053 - 06/30/23 11:01 AM (1 year, 6 months ago)

Quote:

SirPsycho said:
Those weren't sterilized, 90mins at least. And that's after letting steam vent for a minimum of 10 mins





The moment of illumination.
Ok, I feel dumb. It's nice to learn though. Thank you

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