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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
First liquid culture, does this look good so far?
    #28371707 - 06/23/23 07:09 PM (1 year, 6 months ago)

So I know I did this kinda half assed, but I had a spore syringe that was just sitting so I mixed up some water and honey and since I don’t have a pressure cooker I boiled it for 20mins and shot about 8cc of the spore syringe directly into the jar out the top back on and iv been letting it sit it’s been about a week and this is what I have, was I successful or is it contaminated?

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OfflinePandaskis
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209] * 1
    #28371732 - 06/23/23 07:26 PM (1 year, 6 months ago)

Lol the likely hood this isnt contaminated is 0

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Invisibledna24
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209]
    #28371889 - 06/23/23 09:16 PM (1 year, 6 months ago)

Quote:

Myconewbie209 said:
So I know I did this kinda half assed, but I had a spore syringe that was just sitting so I mixed up some water and honey and since I don’t have a pressure cooker I boiled it for 20mins and shot about 8cc of the spore syringe directly into the jar out the top back on and iv been letting it sit it’s been about a week and this is what I have, was I successful or is it contaminated?




LC isnt made by shooting spores into a growable medium


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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: First liquid culture, does this look good so far? [Re: dna24]
    #28371891 - 06/23/23 09:20 PM (1 year, 6 months ago)

That's contaminated.

Get yourself an SAB, everything should be done inside your SAB to prevent contamination:
https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888

Start with Spore Syringe -> Brown Rice Flour. You have some reading to do.
PFtek: https://www.shroomery.org/forums/showflat.php/Number/24179086
Water Tub tek: https://www.shroomery.org/forums/showflat.php/Number/26601054

Then once you have fruits pick out a nice looking one and clone it onto agar.
It's easy to clone, just cut out mycelia from the middle of the stem(aka the "Stipe") and place it on agar to grow.
You can then use this agar culture to make grain jars that wont mold out because you used clean agar - or we assume you will use clean agar that's up to you.

agar: https://www.shroomery.org/forums/showflat.php/Number/19208976
and why agar: https://www.shroomery.org/forums/showflat.php/Number/28188696#28188696

You can gather spore on a print like so: https://www.shroomery.org/forums/showflat.php/Number/24461957
Once you have a print you can use those spores to make more syringes: https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681
Those same spores can also be placed onto agar to continue your cultivation.
Once you have clean agar you can then make liquid culture with that.


--------------------
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Offlinefiddle_head
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209]
    #28371922 - 06/23/23 09:36 PM (1 year, 6 months ago)

Yup. Bout it. 👍


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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Re: First liquid culture, does this look good so far? [Re: mushhead]
    #28378327 - 06/28/23 09:35 PM (1 year, 6 months ago)

So lemme explain exactly what I did, I boiled the jar with the water and honey mixture for about 20-25mins
Let it cool then put my oven to 350 let it warm up once warm I crack the door and I work in the draft of hot air since I don’t have a flow hood or anything
I got my spore syringe wiped it down the iiso popped open the jar injected 8cc into it and closed it back up all while working in that draft of hot air, ima buy a pressure cooker soon and a big clear bin to make a sab but untill then you think I can maybe make some ajar and hopefully get a few clean pieces off of it to work off of to hopfully make something sterile later on
Or should I just toss this jar out

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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,242
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Re: First liquid culture, does this look good so far? [Re: mushhead] * 1
    #28378329 - 06/28/23 09:36 PM (1 year, 6 months ago)

Toss the jar. Follow this specific route quoted below and you will have success.
honestly there's a lot I could dissect that's wrong with your procedure.
You can't put spores to Liquid culture first off, it's dirty as fuck.
Your "sterilization" was useless because it honestly wasn't sterilized at all.
LC needs to be Pressure Cooked at 15PSI for 45min at least for it be thought of as ready for use.
You need to do all your work inside an SAB, none of this standing in front of your oven malarkey.
The reason the oven does not work is that one, it's in the open air, and two the oven itself is causing turbulence which forces contamination into your jar. You basically invited contams into your grow.
Quote:

mushhead said:
That's contaminated.

Get yourself an SAB, everything should be done inside your SAB to prevent contamination:
https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888

Start with Spore Syringe -> Brown Rice Flour. You have some reading to do.
PFtek: https://www.shroomery.org/forums/showflat.php/Number/24179086
Water Tub tek: https://www.shroomery.org/forums/showflat.php/Number/26601054

Then once you have fruits pick out a nice looking one and clone it onto agar.
It's easy to clone, just cut out mycelia from the middle of the stem(aka the "Stipe") and place it on agar to grow.
You can then use this agar culture to make grain jars that wont mold out because you used clean agar - or we assume you will use clean agar that's up to you.

agar: https://www.shroomery.org/forums/showflat.php/Number/19208976
and why agar: https://www.shroomery.org/forums/showflat.php/Number/28188696#28188696

You can gather spore on a print like so: https://www.shroomery.org/forums/showflat.php/Number/24461957
Once you have a print you can use those spores to make more syringes: https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681
Those same spores can also be placed onto agar to continue your cultivation.
Once you have clean agar you can then make liquid culture with that.



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OfflinePandaskis
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209] * 1
    #28378343 - 06/28/23 09:44 PM (1 year, 6 months ago)

Quote:

Myconewbie209 said:
So lemme explain exactly what I did, I boiled the jar with the water and honey mixture for about 20-25mins
Let it cool then put my oven to 350 let it warm up once warm I crack the door and I work in the draft of hot air since I don’t have a flow hood or anything
I got my spore syringe wiped it down the iiso popped open the jar injected 8cc into it and closed it back up all while working in that draft of hot air, ima buy a pressure cooker soon and a big clear bin to make a sab but untill then you think I can maybe make some ajar and hopefully get a few clean pieces off of it to work off of to hopfully make something sterile later on
Or should I just toss this jar out




Listen to mushhead :smile:, appreciate the detailed rundown, but as soon as you said spore syringe to LC we know its contaminated

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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Re: First liquid culture, does this look good so far? [Re: Pandaskis]
    #28378424 - 06/28/23 11:05 PM (1 year, 6 months ago)

So now my question is could I at least use it and put it to agar and hopfully clean it up enough to hopfully get a piece that’s usable to put to grain and mutipul transfers hopfully

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Invisibledna24
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Registered: 04/19/22
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209] * 1
    #28378427 - 06/28/23 11:06 PM (1 year, 6 months ago)

Quote:

Myconewbie209 said:
So now my question is could I at least use it and put it to agar and hopfully clean it up enough to hopfully get a piece that’s usable to put to grain and mutipul transfers hopfully



maybe you can always try

if you still have the mss then you can do that to agar too or just do that


--------------------

Edited by dna24 (06/28/23 11:07 PM)

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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: First liquid culture, does this look good so far? [Re: dna24] * 1
    #28378430 - 06/28/23 11:12 PM (1 year, 6 months ago)

Randal is right, you could try but I doubt anything good can come of it.
I would start from spore on agar or spore to BRF.

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OfflinePandaskis
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Re: First liquid culture, does this look good so far? [Re: mushhead]
    #28378699 - 06/29/23 07:35 AM (1 year, 6 months ago)

You could also put it to brf puck then water agar, might be able to out pace the bacteria that way

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Invisiblemushhead
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Registered: 08/22/14
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Re: First liquid culture, does this look good so far? [Re: Pandaskis]
    #28378704 - 06/29/23 07:43 AM (1 year, 6 months ago)

most likely @Pandaskis his LC solution has much more than just bacteria.

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OfflinePandaskis
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Re: First liquid culture, does this look good so far? [Re: mushhead]
    #28378707 - 06/29/23 07:47 AM (1 year, 6 months ago)

Quote:

mushhead said:
most likely @Pandaskis his LC solution has much more than just bacteria.




Yeahh :/, its unlikely to work, but if hes desperate to do it; thats the best method i can think of :shrug:

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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: First liquid culture, does this look good so far? [Re: mushhead]
    #28378709 - 06/29/23 07:48 AM (1 year, 6 months ago)

I was just going to say, brown bits in LC is a bit on the extreme side for just bact

I can't even think what could cause brown debris in there tbh


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Invisibledna24
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Re: First liquid culture, does this look good so far? [Re: altford78]
    #28378752 - 06/29/23 08:22 AM (1 year, 6 months ago)

Quote:

altford78 said:
I was just going to say, brown bits in LC is a bit on the extreme side for just bact

I can't even think what could cause brown debris in there tbh




thats literally the scariest part of the pics, what on earth is causing that weird brown growth


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InvisibleYuggoth
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209]
    #28378864 - 06/29/23 10:47 AM (1 year, 6 months ago)

Quote:

Myconewbie209 said:
So now my question is could I at least use it and put it to agar and hopfully clean it up enough to hopfully get a piece that’s usable to put to grain and mutipul transfers hopfully




You could but you'd be better off just doing that with spores. The LC is likely to have more contamination than your original spore syringe because it's such a good environment for bacteria.


--------------------
We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

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OfflineMyconewbie209
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Re: First liquid culture, does this look good so far? [Re: Yuggoth]
    #28379538 - 06/29/23 08:51 PM (1 year, 6 months ago)

Those bits are chunks of spores from the syringe and the days so by they are slowly going away as more mycelium forms
I shook the syringe as much as I could but couldn’t break em up all the way

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OfflinePandaskis
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209] * 2
    #28379567 - 06/29/23 09:33 PM (1 year, 6 months ago)

Quote:

Myconewbie209 said:
Those bits are chunks of spores from the syringe and the days so by they are slowly going away as more mycelium forms
I shook the syringe as much as I could but couldn’t break em up all the way




If you shot enough into the LC solution that you can see that many spores, then yeah this LC is fucked. If im honest, it doesnt look like spores to me

Edited by Pandaskis (06/29/23 09:34 PM)

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OfflineCocaineBuffet
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Re: First liquid culture, does this look good so far? [Re: Myconewbie209] * 2
    #28379570 - 06/29/23 09:38 PM (1 year, 6 months ago)

Quote:

Myconewbie209 said: I don’t have a pressure cooker I boiled it for 20mins and shot about 8cc of the spore syringe directly into the jar...




Say less.

Toss it.

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