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OfflineAmnesiac01
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Registered: 06/24/23
Posts: 2
Last seen: 1 year, 4 months
Need some guidance on my first attempt!
    #28378141 - 06/28/23 07:01 PM (1 year, 6 months ago)

So I’ve been reading bout all this for quite some time now but just recently decided to give it a go.

I’ve got 2 five lb. all in one bags that came with mixed substrate and grain. Both were in inoculated 46 days ago with MSS. I broke and shook about 5 weeks in when they were at 30% colonization. They survived the shake but seem to have stalled now, with large amounts of sub on the bottoms uncolonized. I’m attaching pics.





I’ve also got 2 three lb. grain bags inoculated with LC about five weeks ago in similar condition. Survived the break and shake but now they both still have good sized patches of uncolonized grain.









I just wanted to ask if this process should take this long or if something may be wrong. Should I break and shake again? Do I just need more patience? Any help greatly appreciated.

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OfflineMistaMycology
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Registered: 04/23/23
Posts: 44
Last seen: 1 year, 6 months
Re: Need some guidance on my first attempt! [Re: Amnesiac01]
    #28378148 - 06/28/23 07:14 PM (1 year, 6 months ago)

Typically stalling is a sign of bacteria. Myc won’t grow where bacteria is present. You can try another shake if it doesn’t recover I would deem the bags bacterial. On a side note I would really advise staying away from all in one bags a lot of people have issues with them. Do some research on bulk growing or even PF Tek although I’ve never done PF myself it’s tried and true by many people on here. Most people have little luck with all in one bags or anything presterilized. I would also advise learning about agar before your next run. If you combine agar with a bulk tek I can promise you have more success! Just keep doing your research your on the right track and I wish you the best of luck getting any fruits from your all in one bags!

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Invisiblemushhead
Livonia Wanderer
I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Need some guidance on my first attempt! [Re: MistaMycology]
    #28378150 - 06/28/23 07:18 PM (1 year, 6 months ago)

Get yourself an SAB, everything should be done inside your SAB to prevent contamination:
https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888

Start with Spore Syringe -> Brown Rice Flour. You have some reading to do.
PFtek: https://www.shroomery.org/forums/showflat.php/Number/24179086
Water Tub tek: https://www.shroomery.org/forums/showflat.php/Number/26601054

Then once you have fruits pick out a nice looking one and clone it onto agar.
It's easy to clone, just cut out mycelia from the middle of the stem(aka the "Stipe") and place it on agar to grow.
You can then use this agar culture to make grain jars/bags that wont mold out because you used clean agar - or we assume you will use clean agar that's up to you.

agar: https://www.shroomery.org/forums/showflat.php/Number/19208976
and why agar: https://www.shroomery.org/forums/showflat.php/Number/28188696#28188696

You can gather spore on a print like so: https://www.shroomery.org/forums/showflat.php/Number/24461957
Once you have a print you can use those spores to make more syringes: https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681
Those same spores can also be placed onto agar to continue your cultivation.


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OfflineAmnesiac01
Stranger

Registered: 06/24/23
Posts: 2
Last seen: 1 year, 4 months
Re: Need some guidance on my first attempt! [Re: MistaMycology]
    #28378215 - 06/28/23 08:28 PM (1 year, 6 months ago)

Word I really appreciate the quick feedback. Yeah I kinda got the bags before I had done much research and realized a little late it wasn’t a very reliable way to go.. I’ll see if they work out but not holding my breath..
That’s why I was hoping the grain/LC would maybe bring better luck, but based on all I’ve read (which is a lot lol) those are taking too long too. Thanks for the help bud.

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