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Offlinebaldur
Stranger

Registered: 06/30/21
Posts: 434
Last seen: 18 days, 9 hours
My agar plates (from clones) look yellow - senescence?
    #28377873 - 06/28/23 02:05 PM (1 year, 6 months ago)

I have a fourth generation (P4) cloneplate of golden teacher which looked quite healthy, from 20.4.23. Took a transfer from it on the 2.6.23, and before the plate was fully colonized the center part started to look yellow. This happened to about 10 plates or so, all the plates in this batch. Isn't this something to worry about? Does this mean that I'm starting to see the effects of senescence?

The clone was taken from a first generation grow, meaning that it came straight from multispore (MS to AGAR to GRAIN SPAWN JARS to BAGS to TUBS).

I'm not completely familiar with the concept of senescence, but could this be that?

Should I be ok with using this plate?

Note that this is just one plate out of many, all which are showing the same yellowing.

Here is a picture of the orginal clone plate, quite healthy I'd say.



And here is an image of the yellow daughter plate. Note that the yellowing is a bit stronger than the picture suggests.


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OfflinePandaskis
Eating Bamboo
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Registered: 03/14/23
Posts: 1,869
Last seen: 8 months, 8 days
Re: My agar plates (from clones) look yellow - senescence? [Re: baldur]
    #28377874 - 06/28/23 02:10 PM (1 year, 6 months ago)

Ive never read of senescence causing yellowing. I would send the plate and see what happens, but please do wait for a second opinion! :smile: :takingnotes:

Edited by Pandaskis (06/28/23 02:11 PM)

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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: My agar plates (from clones) look yellow - senescence? [Re: Pandaskis]
    #28377876 - 06/28/23 02:12 PM (1 year, 6 months ago)

I get that once plates hit around 1month old. I always just thought it was drying up :shrug:


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Offlinephlanx
Experimentor


Registered: 06/11/05
Posts: 191
Loc: U.S.
Last seen: 4 months, 24 days
Re: My agar plates (from clones) look yellow - senescence? [Re: baldur]
    #28377952 - 06/28/23 03:35 PM (1 year, 6 months ago)

Hey Baldur,

So senescence should take quite a few transfers over the course of many months or even years.  Looks like your plates are drying out a little bit.

If you constantly take clones to fruit to clone to fruit or g2g to g2g to g2g constantly expanding myc without ever going back to spore or back to a culture to revitalize, you can reach senescence. 

Reduced vigor is generally the term people use when they see senescence, it means slow or no growth or failure to take up nutrients over time.

-phlanx

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Offlinebaldur
Stranger

Registered: 06/30/21
Posts: 434
Last seen: 18 days, 9 hours
Re: My agar plates (from clones) look yellow - senescence? [Re: phlanx]
    #28378069 - 06/28/23 05:40 PM (1 year, 6 months ago)

Ahh ok I thought senescence was reached much earlier than after multiple multiple grows!

It's just that I've worked with quite some numbers of agar plates in the past, and never seen this. And all my plates are showing this coloring. But this is the first time that I work with clones... which is why I thought this might be senescence.

Edited by baldur (06/28/23 05:40 PM)

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