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InvisiblePsillySeeEms
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Registered: 11/12/22
Posts: 1,523
Loc: PNW
Re: Home made laminar flow with $100 [Re: johnukguy]
    #28376545 - 06/27/23 12:46 PM (1 year, 6 months ago)

Nm the corsi-rosenthal 4-way DIY air filter...

What about a legit 4-way LFH box. Using the proper filters on 4 sides, with the appropriate oversized blower motor hooked to the top mounted plenum? Design the whole work table it sits on to spin like a lazy susan, so you don't even have to get up.

I guess you could sit in an office chair and be surrounded by multiple flow hoods instead, and get dizzy spinning in circles.

:grin:

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OfflineGlomus
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Registered: 06/24/23
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Re: Home made laminar flow with $100 [Re: SirPsycho]
    #28376804 - 06/27/23 05:21 PM (1 year, 6 months ago)

I just built it this week so give me some more time, but first pic is an agar plate sitting in front of this thing for 3 minutes with the lid off. Second pic is Turkey tail culture from a LC.


^^^^ Look up ^^^^^

The first sign of contamination, I'll post it here. Check out the video I posted, he does a full grow from spores to fruiting mushrooms with several strains using this exact same design.

Edited by Glomus (06/27/23 05:23 PM)

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OfflineSirPsycho
Purple Belt in Google-Fu
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Registered: 01/01/20
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Re: Home made laminar flow with $100 [Re: Glomus] * 1
    #28376807 - 06/27/23 05:24 PM (1 year, 6 months ago)

Quote:

Glomus said:
I just built it this week so give me some more time, but first pic is an agar plate sitting in front of this thing for 3 minutes with the lid off. Second pic is Turkey tail culture from a LC.


^^^^ Look up ^^^^^



One plate isn't exactly impressive. I used to have 100% succes rate with open air transfers until recently. 2 for 2 clean results out of contamed donor plates. Now I'm at 66.666666...%:wink:

Let's see some bags and a smoke test.


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

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OfflineGlomus
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Re: Home made laminar flow with $100 [Re: SirPsycho]
    #28376820 - 06/27/23 05:39 PM (1 year, 6 months ago)

I did do a smoke test with an incent already, mainly to check for leaks around the seals, but I did see constant streams about an inch in from the edges. I'll post a vid doing that again and see how well it turns out. I'll admit I probably only have about 6-7 inches of laminar flow from the filter tho.

And like I said I just built it a week ago using the same guidelines as the guy in that video I posted, who had clean cultures and controls as well as running a particle device in front of it that showed 0ppm

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OfflineSirPsycho
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Re: Home made laminar flow with $100 [Re: Glomus] * 1
    #28376879 - 06/27/23 06:15 PM (1 year, 6 months ago)

Quote:

Glomus said:
I'll admit I probably only have about 6-7 inches of laminar flow from the filter tho.




So wtf is the point then? Your effective work area is so much smaller that using the same tub as an SAB.

Also if you're just injecting through SHIPs you will have just as much success in open air. The vectors for that aren't the air cause media doesn't get exposed to air, it's your tools.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineTri
Paramecium Brain
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Registered: 08/23/18
Posts: 1,206
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Re: Home made laminar flow with $100 [Re: SirPsycho]
    #28377268 - 06/28/23 01:37 AM (1 year, 6 months ago)

I mentioned it before but ill actually post the link this time, if you're injecting bags, check this out Sterilizing SHIPs after an ISO wipe


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𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

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OfflineGlomus
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Registered: 06/24/23
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Re: Home made laminar flow with $100 [Re: Tri]
    #28377302 - 06/28/23 03:16 AM (1 year, 6 months ago)

Yeah, I've injected into SHIPs in open air and never had problems, doing LC on a bigger scale is a bit new for me. Planning to test my LC with agar before using each jar. Anything you can add to your process to isolate the possible cause of contamination is constructive.

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OfflineGlomus
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Re: Home made laminar flow with $100 [Re: SirPsycho]
    #28377303 - 06/28/23 03:22 AM (1 year, 6 months ago)

I just found a small cobb web mold spot on the edge of the agar in my control culture today. However that was before I really found the clear area of constant flow of the hood and wasn't using a proper rack to elevate my culture in front of the filter. Today I did a thorough smoke test on it and marked with a sharpie on the filter area where my constant flow is. 
I'm not gona give up on this experiment and do some more controls, my other cultures are fine I think cuz I opened slightly for as short as possible to inoculate them. Where as control agar plate was open for 3 minutes.

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OfflinePandaskis
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Re: Home made laminar flow with $100 [Re: Glomus] * 2
    #28377325 - 06/28/23 04:30 AM (1 year, 6 months ago)

:tryingnottodie:

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