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OfflineSirPsycho
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Re: Halos and water droplets. What’s going on? [Re: MicroMycelium] * 3
    #28375827 - 06/26/23 08:31 PM (6 months, 29 days ago)



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InvisibleBigwormS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 6
    #28375908 - 06/26/23 09:54 PM (6 months, 29 days ago)

Just fixing the title. Please don't change the subject line!


Edited by Bigworm (06/26/23 09:55 PM)


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InvisibleStipe-n CapMDiscord
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Loc: Canada
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm]
    #28376141 - 06/27/23 06:18 AM (6 months, 29 days ago)

:lol:


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Invisiblepablotx
pipette monkey


Registered: 05/06/23
Posts: 45
Loc: Hot & humid
Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28376307 - 06/27/23 09:34 AM (6 months, 29 days ago)

Pan cyan Jamaica MS. Any advice?



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Offlineeugenemyco
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Registered: 01/30/23
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Re: AGAR ENVY! (Anything and All things agar!) [Re: pablotx]
    #28376342 - 06/27/23 10:01 AM (6 months, 29 days ago)



Sad rhizo plate completely overlaid with mold


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: AGAR ENVY! (Anything and All things agar!) [Re: eugenemyco] * 2
    #28376436 - 06/27/23 11:17 AM (6 months, 29 days ago)

Proofing the rustywhytes today!

Here are the chosen plates, they'll be going to grain today.
I'm pretty sure they're rustys but we still gotta proof our cultures before running a whole load a of grain.
Now before you say anything about how much parafilm I have used, I have changed my ways and no longer use so much.
These plates were just done before the change was made. :cheers:


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Funguy50]
    #28376548 - 06/27/23 12:48 PM (6 months, 29 days ago)

Quote:

Funguy50 said:
Quote:

SirPsycho said:
Blank or inoculated?



Blank for now but knowing for both would be good.



I've stored both in the fridge.

Blanks I pour, let cool to room temp, then stack ten, wrap micropore tape over it twice (once in each direction) then put it back in the sleeve it came in and tape the top down. I usually keep those a week before using to make sure none are dirty, and can toss any that are. It's rare but happens. I stack them up on the floor and toss a clean T-shirt over them to keep dust off them.

Any I don't use within 3 weeks I put in the fridge. Keep fridge just above freezing. between 32 F and 34 F.  I keep them upside down so any condensation goes in the top (bottom) not on the agar. They can be used a month after that, but will eventually dry out. I've used them after 2 months in the fridge, but don't like to.

Plates that I've grown but want to keep, I just seal up and put in a zip lock and put in the fridge. Write on the zip lock what they are. (date, species, variety, F / T).

The cool creepy thing is they will grow in the fridge. VERY slowly. Like in 6 months they'll grow what they'd grow in the room in a week.

I've transferred from and revived a (cube) line from just over a year in the fridge. Some people have revived more than that.

You can transfer from them as soon as you take them out, no need to let go to room temp.

Some people are doing some longer-term storage now with water or some crazy voodoo like that but I haven't done it.


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OfflineMonoVision
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Registered: 02/14/23
Posts: 76
Last seen: 3 months, 23 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28376981 - 06/27/23 07:30 PM (6 months, 28 days ago)

So I’ve got these plate pins(plins) or whatever you want to call them. Since these are sealed in the dish they should theoretically be sterile, correct?  Should I still split down the middle and take a sample from the middle to clone? Or can I just cut a section off and throw it on a new plate and call it a day?



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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision] * 1
    #28376995 - 06/27/23 07:40 PM (6 months, 28 days ago)

With plins I just use the whole thing if it's small. Large ones I'll cut a cross section of the stipe. Being in a steril environment no need to take a center sample.


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InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh πŸ˜›
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision] * 2
    #28377008 - 06/27/23 07:49 PM (6 months, 28 days ago)

Quote:

MonoVision said:
So I’ve got these plate pins(plins) or whatever you want to call them. Since these are sealed in the dish they should theoretically be sterile, correct?  Should I still split down the middle and take a sample from the middle to clone? Or can I just cut a section off and throw it on a new plate and call it a day?





Transfer the entire pin and then transfer the mycelium that's grows from the new pin plate. :thumbup:


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OfflineMonoVision
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Registered: 02/14/23
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Last seen: 3 months, 23 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28377028 - 06/27/23 08:01 PM (6 months, 28 days ago)

Awesome! Thanks y’all :iloveyou:


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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MonoVision]
    #28377059 - 06/27/23 08:18 PM (6 months, 28 days ago)

Quote:

MonoVision said:
Awesome! Thanks y’all :iloveyou:



To clone a plin, slice the plin above the bottom of its little stem to avoid getting any non-clone mycelium from the agar with it if you want a clone only, which you probably do. Do it with a plate where no plins have dropped spores yet. This is the easiest way to clone, and less likely to have contam than cutting from a shroom growing in the open air.

Then use your scalpel to push it into a clean plate.

Plin pushed into a fresh plate

Everything I've grown in the past two years has been the fruit of a single plate plin of x7x which produced consistently strong shrooms, or of a tall pheno line off that. I lucked out, was my first try, and was stronger than what it grew from.

But the theory is that first plins are often robust genetics since they grow out of sub-ideal conditions and wave their little flags around first. As in "the force is strong in this one." 

-

Plins aka plate pins ready to be cloned to a fresh plate.



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Edited by nektar61 (06/27/23 08:34 PM)


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OfflineTri-Polar
Paramecium Brain
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Registered: 08/23/18
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #28377232 - 06/28/23 12:16 AM (6 months, 28 days ago)



Exposed to open air/ AC blowing on them for ~30 seconds at 2% 1% and .2% nutes.

Not feeling warm and fuzzy about nothing showing up at all yet, been having shit luck with agar and im thinking I might need to lower the agar concentration from 2%

Any words of wisdom?


--------------------
Intro to Shroomery:raver2:The Sexy TEKsβ„’

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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar]
    #28377235 - 06/28/23 12:21 AM (6 months, 28 days ago)

Quote:

Tri-Polar said:


Exposed to open air/ AC blowing on them for ~30 seconds at 2% 1% and .2% nutes.

Not feeling warm and fuzzy about nothing showing up at all yet, been having shit luck with agar and im thinking I might need to lower the agar concentration from 2%

Any words of wisdom?




You're trying to see if you can grow contam from your home air, right? How long did you let it sit after exposure?

If it's a week, try spitting in them when you just woke up before brushing your teeth.

I am no expert on Agar, but I've got some things worked out that work consistently for me:
https://www.shroomery.org/forums/showflat.php/Number/27551422#27551422

What variety (maybe even species for right) shrooms are in your sig line? They look very nice.


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OfflineTri-Polar
Paramecium Brain
I'm a teapot User Gallery


Registered: 08/23/18
Posts: 374
Loc: purgatory
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28377240 - 06/28/23 12:37 AM (6 months, 28 days ago)

Oh shoot thought I mentioned, been 4.5 days, and I have an insane sporeload(moldy apartment building + pnw) so I know shits on there.

Good writeup, I am personally somewhat partial to MEA even though I've been fucking it up a lot recently lol, im drawn to toxic relationships  :shrug: :rofl:

I read a thread (the "no pours suck" one) and it seemed like the OP had a lot of contams that weren't showing up due to seemingly his agar being too hard, lowered it to 1.25% or something and found so much trouble with contam I think they denounced SABs and quit the hobby lmao, rough stuff. But from that thread I've been inspired to 1. Lower my agar concentration and see if im secretly harboring nasties and 2. Create a time-lapse of particulates in an SAB

Also my sig is TW and Hillbilly pumpkin :biggrin:


--------------------
Intro to Shroomery:raver2:The Sexy TEKsβ„’

IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH.

hey fuck you dont look at me


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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar]
    #28377260 - 06/28/23 01:17 AM (6 months, 28 days ago)

Quote:

Tri-Polar said:
Oh shoot thought I mentioned, been 4.5 days




Bacteria often shows on a plate in 3 days, but can take longer. Mold (microscopic fungi) can take a week. That's why I mentioned yesterday letting plates sit a week after pouring, to see if anything grows. Saves occasional problems after nocing.

Put those plates on a heating pad. That always gives noobs contams when they put one under a shoebox. haha.

Thank you for the compliment. I try.

I had better luck early on with grocery store potato flakes than malt extract. Might have been beginner's luck, but I stayed with it.


--------------------
-NEW? Start here.


Edited by nektar61 (06/28/23 01:25 AM)


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Invisiblemilkboy
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Registered: 05/01/21
Posts: 2,295
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #28377265 - 06/28/23 01:30 AM (6 months, 28 days ago)

Naw potato flake agar is the shiz, all the cool kids are doing it


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OfflineSirPsycho
Purple Belt in Google-Fu
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar] * 2
    #28377342 - 06/28/23 05:04 AM (6 months, 28 days ago)

Quote:

Tri-Polar said:


Exposed to open air/ AC blowing on them for ~30 seconds at 2% 1% and .2% nutes.

Not feeling warm and fuzzy about nothing showing up at all yet, been having shit luck with agar and im thinking I might need to lower the agar concentration from 2%

Any words of wisdom?



How long since exposure? Nvm I should read the rest of the thread before posting.

Still a touch early imo


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Invisiblealtford78
What do I put here

Registered: 05/09/23
Posts: 890
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar]
    #28377347 - 06/28/23 05:16 AM (6 months, 28 days ago)

Quote:

Tri-Polar said:
Oh shoot thought I mentioned, been 4.5 days, and I have an insane sporeload(moldy apartment building + pnw) so I know shits on there.

Good writeup, I am personally somewhat partial to MEA even though I've been fucking it up a lot recently lol, im drawn to toxic relationships  :shrug: :rofl:

I read a thread (the "no pours suck" one) and it seemed like the OP had a lot of contams that weren't showing up due to seemingly his agar being too hard, lowered it to 1.25% or something and found so much trouble with contam I think they denounced SABs and quit the hobby lmao, rough stuff. But from that thread I've been inspired to 1. Lower my agar concentration and see if im secretly harboring nasties and 2. Create a time-lapse of particulates in an SAB

Also my sig is TW and Hillbilly pumpkin :biggrin:




Backlight them, do you still see nothing?
You have a whole range of nutrient % in there, something should find one of them hospitable.

I mean contam will even show up on water agar eventually so something's up here if nothing grows by tomorrow/after tomorrow.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes


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InvisibleGuerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78] * 7
    #28377348 - 06/28/23 05:20 AM (6 months, 28 days ago)



TWA, Gandalf x Omni, Makilla Gorilla x MAK


--------------------
Being pissed on does not make you a real man.


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