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OfflineMyconewbie209
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Registered: 04/03/23
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Colonizing grain out of a tub?
    #28374300 - 06/25/23 04:12 PM (1 year, 6 months ago)

So I have something Iโ€™m curious about, I have a shoebox going already my question is can I colonize grain or keep this going in anyway if I pick out a few pieces of grain that have mycelium on em and throw em in a new grain bag? Or if I pull a chunk of it out and put it in new medium would it recolonize a new grain tub?
Iโ€™m new to this so I apologize for the noob questions

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OfflineSpirit-Crusher
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Re: Colonizing grain out of a tub? [Re: Myconewbie209] * 1
    #28374372 - 06/25/23 05:38 PM (1 year, 6 months ago)

You can do grain to grain transfers. However that has to be done in a sterile setting,.Spawning isn't a sterile process. That ship has sailed for you. You could potentially put a piece on agar, but you'd have to clean the shit out of it.

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OfflineSirPsycho
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Re: Colonizing grain out of a tub? [Re: Spirit-Crusher]
    #28374405 - 06/25/23 06:07 PM (1 year, 6 months ago)

No, not out of a tub. Needs do be done from sealed jars before spawning


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OfflineMyconewbie209
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Re: Colonizing grain out of a tub? [Re: Spirit-Crusher]
    #28374955 - 06/26/23 07:42 AM (1 year, 6 months ago)

You mean maybe set a piece of agar in the tub so that the mycelium starts to colonize onto it? Iv never worked with agar but just ordered some
What do you mean exactly by clean it

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Invisiblestroopwafelman
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Registered: 03/21/23
Posts: 386
Re: Colonizing grain out of a tub? [Re: Myconewbie209] * 1
    #28374966 - 06/26/23 07:54 AM (1 year, 6 months ago)

By "cleaning" they mean multiple transfers between agar plates to remove any possible contaminants that may have hitched a ride along with the sample.

In your case however it's a bit too late for taking grain samples to agar since it no longer in a sterile environment when it gets to the tub stage.

And setting a piece of agar in the tub:
A. Isn't a technique for agar work
B. Won't yield anything but contamination as agar work needs to be done is as sterile of an environment as possible and as I mentioned above the tub stage is not sterile.

Your options are:
A. reading up on agar and going with spores
B. reading up on agar (can't get away without it) and taking a samples from a mushroom that grows in your tub to make clones
C. doing a PF-Tek grow from spores and not having to deal with agar or grains but getting much lower yields

Edited by stroopwafelman (06/26/23 08:00 AM)

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OfflinePBJ710
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Re: Colonizing grain out of a tub? [Re: Myconewbie209]
    #28375017 - 06/26/23 08:55 AM (1 year, 6 months ago)

Quote:

Myconewbie209 said:
You mean maybe set a piece of agar in the tub so that the mycelium starts to colonize onto it? Iv never worked with agar but just ordered some
What do you mean exactly by clean it




No, he means taking some sterile tweezers/tool and taking a piece of the grain out and put it on a sterile agar petri.  This will likely grow out both contams and mycelium since your spawn has already been exposed to the outside world, but you might be able to seperate the two through however many transfers it takes and then send it to grain.  It will be an uphill battle though and not many people will recommend it as a preferred method.

Cleaning is the process of seperating clean mycelium away from contams on agar.

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OfflineMyconewbie209
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Registered: 04/03/23
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Re: Colonizing grain out of a tub? [Re: PBJ710]
    #28375738 - 06/26/23 07:31 PM (1 year, 6 months ago)

Thank you guys Iโ€™m going to do some research, I also have some lc that I made well I think I made it right so ima try from there, I put some water and honey ina jar I dnt have a pressure cooker so I boiled it for about 30 mins and I donโ€™t have a sab yet so what I do is turn on the oven 450 wipe my spore syringe down with iso and cracked open the oven and worked in the draft of hot air to put 8cc of my spore syringe into the jar and now itโ€™s starting to look like itโ€™s colonizing but ima move it to agar and hope I can clean it up and get some clean mycelium out of it

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OfflineMyconewbie209
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Registered: 04/03/23
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Re: Colonizing grain out of a tub? [Re: Myconewbie209]
    #28375740 - 06/26/23 07:32 PM (1 year, 6 months ago)


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