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Shroomancer
Stranger



Registered: 05/04/23
Posts: 12
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Liquid culture sediment or mycelium?
#28373667 - 06/25/23 05:04 AM (1 year, 6 months ago) |
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It's been exactly one week since I injected my LC with Spores and there has been a sediment like substance growing at the bottom. This is my first attempt at LC so idk what Mycelium would look like in LC so I'm not able to tell what it is. The LC is made of honey and distilled water. I used a spore syringe to inoculate it as Agar is outrageously expensive when I live. The first image is the first day of inoculation and the rest are from today.
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Sockadin



Registered: 01/03/10
Posts: 7,266
Last seen: 1 month, 22 days
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Re: Liquid culture sediment or mycelium? [Re: Shroomancer]
#28373671 - 06/25/23 05:09 AM (1 year, 6 months ago) |
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Ita really hard to tell, spores to LC is always a recipe for disaster. Mushrooms fruit in open air so when you print them there will always be air born contamination on the print. It's better to go with spores to agar, a few transfers to clean it up and then agar to LC.
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Shroomancer
Stranger



Registered: 05/04/23
Posts: 12
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Re: Liquid culture sediment or mycelium? [Re: Sockadin]
#28373673 - 06/25/23 05:11 AM (1 year, 6 months ago) |
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Yeah I know, as I stated in my post where I'm living they're really overpriced which is why I wanna try get by without having to use any
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PBJ710
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Registered: 07/05/19
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Re: Liquid culture sediment or mycelium? [Re: Shroomancer] 2
#28373717 - 06/25/23 06:06 AM (1 year, 6 months ago) |
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I find it hard to believe that the cost of agar is so high that it justifies using methods that are so failure prone. Even if it costs 10x as much as the North American prices, it's still worth it.
An ounce of agar will make 35-40 petris and that should be enough to get a few strains cleaned up and sent to grain.
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Shroomancer
Stranger



Registered: 05/04/23
Posts: 12
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Re: Liquid culture sediment or mycelium? [Re: PBJ710]
#28373767 - 06/25/23 07:14 AM (1 year, 6 months ago) |
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I don't know anything about making Agar though, so this just seemed like the easier option
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Pandaskis
Eating Bamboo



Registered: 03/14/23
Posts: 1,869
Last seen: 8 months, 8 days
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Re: Liquid culture sediment or mycelium? [Re: Shroomancer]
#28373774 - 06/25/23 07:17 AM (1 year, 6 months ago) |
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Quote:
Shroomancer said: I don't know anything about making Agar though, so this just seemed like the easier option
If you can make liquid culture you can make agar. You literally just use the liquid culture recipe + a bit of agar.
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Shroomancer
Stranger



Registered: 05/04/23
Posts: 12
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Re: Liquid culture sediment or mycelium? [Re: Pandaskis]
#28373794 - 06/25/23 07:34 AM (1 year, 6 months ago) |
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Oh okay yeah I'll do that then if this doesn't work out which I think will probably happen
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mushhead
Livonia Wanderer



Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
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Re: Liquid culture sediment or mycelium? [Re: Shroomancer] 1
#28373803 - 06/25/23 07:40 AM (1 year, 6 months ago) |
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This will for sure not work. Most likely those spores were covered in Bacteria or mold spores too. The Liquid Culture gives the bacteria free reign over the solution because they're motile in liquids and any mycelia you grow will be bacterial. Even super moldy. No Doubt. Your attempts to grow cheaply like this will fail. I'm sorry to say.
Get yourself an SAB, everything should be done inside your SAB to prevent contamination: https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888
Start with Spore Syringe -> Brown Rice Flour. You have some reading to do. PFtek: https://www.shroomery.org/forums/showflat.php/Number/24179086 Water Tub tek: https://www.shroomery.org/forums/showflat.php/Number/26601054
Then once you have fruits pick out a nice looking one and clone it onto agar. It's easy to clone, just cut out mycelia from the middle of the stem(aka the "Stipe") and place it on agar to grow. You can then use this agar culture to make grain jars that wont mold out because you used clean agar - or we assume you will use clean agar that's up to you.
agar: https://www.shroomery.org/forums/showflat.php/Number/19208976 and why agar: https://www.shroomery.org/forums/showflat.php/Number/28188696#28188696
You can gather spore on a print like so: https://www.shroomery.org/forums/showflat.php/Number/24461957 Once you have a print you can use those spores to make more syringes: https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681 Those same spores can also be placed onto agar to continue your cultivation. Once you have a clean culture on agar you can then use that to make Liquid Culture.
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Baba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,496
Loc: Try 'n' Error Boot Camp
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Re: Liquid culture sediment or mycelium? [Re: mushhead]
#28373837 - 06/25/23 08:27 AM (1 year, 6 months ago) |
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If it was clear before and is showing turbidity like this now then it's 100% contaminated. Photo below is showing a clean vs. contaminated LC.

If you don't invest in agar then you will loose a lot of money with contaminated grain. Also next time you are doing LC in a jar then don't fill it up to the top like this, only fill it half way to keep the lid dry and make a better lid. Use unmodded lids or a lids with a propper filter setup. This tinfoil, rubber band, lid contraption of yours alone could be the reason for a failing LC.
What were you planning to use this LC on? Have you got a PC? I would recommend you take it slow and limit yourself to PF-Tek as recommended above by mushhead. At least for the beginning.
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PBJ710
Strangler


Registered: 07/05/19
Posts: 3,301
Last seen: 5 hours, 48 minutes
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Re: Liquid culture sediment or mycelium? [Re: Shroomancer] 1
#28373882 - 06/25/23 09:05 AM (1 year, 6 months ago) |
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Quote:
Shroomancer said: I don't know anything about making Agar though, so this just seemed like the easier option
Do you want easy or do you want to be successful? The 2 are often mutually exclusive...
If you want to stay low tech, make your own PFTek cakes and use the MS syringes on them as they are somewhat resistant to the bacteria typically present in syringes both due to the structure PFteks provide as well as not being broken up and redistributing any latent contams/weakening the mycelium.
If you want to use grains and do tubs, the cultures need to be 100% axenic or contams will take over with the grain being broken up repeatedly. For this to work, you need to learn about agar so you can clean up the culture before allowing it to expand to grains.
IMO people shouldn't even attempt to make LC until they have at least a decent understanding of agar use or they will waste LOADS of time, grain and substrates. You will need to test the LC on agar anyhow, so it's kind of one of those walk before you run things.
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