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OfflineArctic_Fox
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Pressure cooking fail
    #28373344 - 06/24/23 08:06 PM (1 year, 6 months ago)



The thing that I was using to elevate the petri dishes vibrated into a hole and fell over and ruined the batch.

:canthelpbutlaugh:


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InvisibleYuggoth
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Re: Pressure cooking fail [Re: Arctic_Fox] * 1
    #28373352 - 06/24/23 08:15 PM (1 year, 6 months ago)

I have the same wobbly steamer basket. This was $12 well spent.

https://www.amazon.com/dp/B078KVXQYC


--------------------
We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

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Invisibledna24
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373413 - 06/24/23 09:19 PM (1 year, 6 months ago)

Quote:

Arctic_Fox said:


The thing that I was using to elevate the petri dishes vibrated into a hole and fell over and ruined the batch.

:canthelpbutlaugh:





what is that thing? you cant just use jar lids? or the rack the pc comes with? ive done a double rack setup before by using the one from the instantpot


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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: dna24]
    #28373437 - 06/24/23 09:52 PM (1 year, 6 months ago)

It didn't come with a rack other than the one that keeps things a centimeter off the bottom.


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Invisibledna24
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373448 - 06/24/23 09:59 PM (1 year, 6 months ago)

is it a rack or a metal type plate thing with holes in it? my presto has the plate type which ya isnt that high up but you can stick metal mason jar lids under that and raise it up so you run your plates.


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Invisibledna24
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373455 - 06/24/23 10:03 PM (1 year, 6 months ago)

like this


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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: dna24]
    #28373459 - 06/24/23 10:06 PM (1 year, 6 months ago)

Yeah, that's what I've got. Mine's a Presto too.


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OfflineBajazly
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Re: Pressure cooking fail [Re: dna24]
    #28373461 - 06/24/23 10:12 PM (1 year, 6 months ago)

Stack a couple jar bands or some smashed down aluminum can under the trivet that came with the PC and all will be fine. Use 3 stacks/cans for easy balance.  You could also use 3 half pint jars to space it up. This was a rookie mistake.

Edit: You could probably put the trivet on top of the steamer basket and be fine too.

Edited by Bajazly (06/24/23 11:33 PM)

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373463 - 06/24/23 10:14 PM (1 year, 6 months ago)

At very least wrap them in foil. I'd invest in one of these:

https://www.fishersci.ca/shop/products/fisherbrand-sterilizer-box-petri-dishes/03460#

Sterilize them while empty, then pour.

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InvisiblePickle Rick
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373499 - 06/24/23 11:05 PM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
At very least wrap them in foil. I'd invest in one of these:

https://www.fishersci.ca/shop/products/fisherbrand-sterilizer-box-petri-dishes/03460#

Sterilize them while empty, then pour.




these are awesome, I got some cheap ones from aliexpress. they also free'd up space in my pc, as I dry oven sterilize these days


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Offlinesonson
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Re: Pressure cooking fail [Re: Pickle Rick]
    #28373535 - 06/24/23 11:55 PM (1 year, 6 months ago)

I use five empty cans(from some canned seafood :smile: )  to keep the ideal rack height.



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Edited by sonson (06/25/23 12:05 AM)

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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373797 - 06/25/23 07:36 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
Sterilize them while empty, then pour.



Why not sterilize them after pouring? Then there's no chance of contamination.

Also $130 for some sheet metal is exactly what I expect from science suppliers. Why do they do it?


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Edited by Arctic_Fox (06/25/23 07:38 AM)

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373805 - 06/25/23 07:42 AM (1 year, 6 months ago)

You can find them cheaper, the link was just for example. Doing "no pour" glass petris is possible but seems like a pita. Pouring plates is easy af, ivenever contaminated a petri dish due to pouring, ever.

Dry sterilization seems the way to go, like pickle rick said.

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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373811 - 06/25/23 07:49 AM (1 year, 6 months ago)

I'm just thinking that if I have to sterilize the agar anyway, why not do it after pouring.

And you can sterilize in an oven? Why do people use pressure cookers then?


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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373814 - 06/25/23 07:52 AM (1 year, 6 months ago)

Only the glass petris, you can't use the oven for anything else. Dry heat is ineffective. Dry oven heat is sufficient for the nonporous glass petris, the oven will greatly reduce the risk of condensation.  Sterilizing "no-pour" petris in the pc means you'll have to be very careful not to disturb anything while the agar sets in the pc. You'll likely have very annoying levels of condensation as a result.

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap] * 1
    #28373828 - 06/25/23 08:15 AM (1 year, 6 months ago)

I do no pour with PP5 petris. Condensation is really the biggest problem you can run into but I've taken to using a couple of empty plates on the top of each stack for the pc cycle then just leaving the sterilized plates unwrapped in my culture drawer until I use them. They usually dry up in a day or two. Haven't had any contam issues either leaving them unwrapped.

Sterilizing agar is also usually the last thing I do for the day and after I turn the heat off I just go to bed everything is set by the morning. I do wrap the stacks in foil for the pressure cooker cycle just so they are easy to handle. Foil gets carefully removed after and they get gently placed in the drawer.

If you're gonna leave your plates unwrapped don't go waving them around and shit.


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373830 - 06/25/23 08:19 AM (1 year, 6 months ago)

No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373832 - 06/25/23 08:26 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.



Mine are 100x15mm vented petris


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373843 - 06/25/23 08:33 AM (1 year, 6 months ago)

Pp5 petris exist? :mindblown:

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373848 - 06/25/23 08:38 AM (1 year, 6 months ago)

Yep, not as clear as glass but overall pretty great. Although someone did show me some very clear pp5 petris recently

Upside down:


Right side up:


Vent foot inside edge of lid:


They do also get scratched up by scalpels and other tools.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373849 - 06/25/23 08:38 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.



QFT.  I’ve been seeing people no pour glass lately and been like wtf?

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Mycolorado]
    #28373854 - 06/25/23 08:45 AM (1 year, 6 months ago)

Quote:

Mycolorado said:
Quote:

Stipe-n Cap said:
No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.



QFT.  I’ve been seeing people no pour glass lately and been like wtf?



Honestly the reason I'm still doing no pour is purely just due to timing. I don't have a dedicated grow room, I keep my SAB in my bedroom closet with spare substrate materials sorted in it, put it on my dresser when I need it. It's a whole production to set up for sterile work.

Much easier to do no pour and let it set overnight and after going through all sorts of containers I can say petris are actually the best. Although the one thing I didn't try was alien's holy grail type containers, they seem really handy.

From my discussions with people using glass petris I'd imagine they are a bit tougher since the lids don't seem to fit as snugly and move around a lot but on the other hand they usually aren't vented.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: Mycolorado]
    #28373858 - 06/25/23 08:48 AM (1 year, 6 months ago)

Very cool. I'm set in my ways, pouring seems like much less of a pain. We have so many legit options now that jerry rigged, less than ideal options will hopefully be phased out over time. I couldn't imagine dealing with k cups, for instance.

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373859 - 06/25/23 08:50 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
Very cool. I'm set in my ways, pouring seems like much less of a pain. We have so many legit options now that jerry rigged, less than ideal options will hopefully be phased out over time. I couldn't imagine dealing with k cups, for instance.



Yes, I'd bet pouring is way easier and less work overall.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373861 - 06/25/23 08:51 AM (1 year, 6 months ago)

Lol, wtf
I can't see Mycolorado's post...

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373862 - 06/25/23 08:52 AM (1 year, 6 months ago)

So you're the one person ignoring him. Jerk


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflinePandaskis
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373863 - 06/25/23 08:52 AM (1 year, 6 months ago)

Quote:

SirPsycho said:
Yep, not as clear as glass but overall pretty great. Although someone did show me some very clear pp5 petris recently

Upside down:


Right side up:


Vent foot inside edge of lid:


They do also get scratched up by scalpels and other tools.




Can you throw me a link to these.... @-@

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InvisibleMycolorado
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373864 - 06/25/23 08:52 AM (1 year, 6 months ago)

For sure, no issue with no pour as technique.  I run no pour pp5 cups for plant TC so not criticizing it.  I just don’t get no pouring glass Petri dishes that have a non sealing lid.  They’re Heavey and typically non stackable in that they slip around.  I’d just sterilize and pour a’la disposable.

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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373865 - 06/25/23 08:53 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
Lol, wtf
I can't see Mycolorado's post...




I can’t see it either…sir and I posted simultaneously when the page clicked over.  Think it has to do with posts per page settings.

Quote:

SirPsycho said:
So you're the one person ignoring him. Jerk



:lol:

Edited by Mycolorado (06/25/23 08:54 AM)

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Pandaskis]
    #28373867 - 06/25/23 08:54 AM (1 year, 6 months ago)

Quote:

Pandaskis said:
Quote:

SirPsycho said:
Yep, not as clear as glass but overall pretty great. Although someone did show me some very clear pp5 petris recently

Upside down:


Right side up:


Vent foot inside edge of lid:


They do also get scratched up by scalpels and other tools.




Can you throw me a link to these.... @-@



12PK Plastic Petri Dishes with... https://www.amazon.ca/dp/B077MPZKTM?ref=ppx_pop_mob_ap_share


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: SirPsycho] * 1
    #28373868 - 06/25/23 08:54 AM (1 year, 6 months ago)

Quote:

SirPsycho said:
So you're the one person ignoring him. Jerk




Nope, had to ignore and then unignore to get things rolling. Fucking shroomery better get it's shit together:lol:

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Mycolorado]
    #28373870 - 06/25/23 08:55 AM (1 year, 6 months ago)

Quote:

Mycolorado said:
For sure, no issue with no pour as technique.  I run no pour pp5 cups for plant TC so not criticizing it.  I just don’t get no pouring glass Petri dishes that have a non sealing lid.  They’re Heavey and typically non stackable in that they slip around.  I’d just sterilize and pour a’la disposable.



Yes, that's another advantage of the pp5 dishes. They have a slightly raised edge on the lid so the bottom of the next dish sits nicely in there and doesn't slide around.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Pressure cooking fail [Re: SirPsycho]
    #28373872 - 06/25/23 08:58 AM (1 year, 6 months ago)

Oh shit, kinda pricey haha, but i guess their reusable so they make up for their cost

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373873 - 06/25/23 08:59 AM (1 year, 6 months ago)

Top edge of lid:


Next plate on top(no lid on top plate):


Two plates stacked... DUH:


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Pandaskis]
    #28373874 - 06/25/23 08:59 AM (1 year, 6 months ago)

Quote:

Pandaskis said:
Oh shit, kinda pricey haha, but i guess their reusable so they make up for their cost



Compared to disposable for sure, compared to glass not so much.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373926 - 06/25/23 10:11 AM (1 year, 6 months ago)

I just wrapped a stack of empty petri dishes in aluminium foil and put them in the oven on 340°F. If I leave them for an hour, they'll be sterile and ready for pouring, right? Do I need to be aware of any special precautions for keeping them sterile after taking them out?


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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373965 - 06/25/23 10:46 AM (1 year, 6 months ago)

The PP5 petris aren't bad but if you use Parafilm, sometimes the film sticks to them. This makes them hard to unwrap without a lot of unwanted hand motions in the SAB, and you need to be careful not to squeeze the middle of the petri where it flexes, since that can suck air in. I've decided to retire mine from sterile work, I found that I like glass better overall.


--------------------
We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo

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