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OfflineArctic_Fox
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Pressure cooking fail
    #28373344 - 06/24/23 08:06 PM (1 year, 6 months ago)



The thing that I was using to elevate the petri dishes vibrated into a hole and fell over and ruined the batch.

:canthelpbutlaugh:


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InvisibleYuggoth
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Re: Pressure cooking fail [Re: Arctic_Fox] * 1
    #28373352 - 06/24/23 08:15 PM (1 year, 6 months ago)

I have the same wobbly steamer basket. This was $12 well spent.

https://www.amazon.com/dp/B078KVXQYC


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We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo

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Invisibledna24
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Registered: 04/19/22
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373413 - 06/24/23 09:19 PM (1 year, 6 months ago)

Quote:

Arctic_Fox said:


The thing that I was using to elevate the petri dishes vibrated into a hole and fell over and ruined the batch.

:canthelpbutlaugh:





what is that thing? you cant just use jar lids? or the rack the pc comes with? ive done a double rack setup before by using the one from the instantpot


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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: dna24]
    #28373437 - 06/24/23 09:52 PM (1 year, 6 months ago)

It didn't come with a rack other than the one that keeps things a centimeter off the bottom.


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Invisibledna24
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373448 - 06/24/23 09:59 PM (1 year, 6 months ago)

is it a rack or a metal type plate thing with holes in it? my presto has the plate type which ya isnt that high up but you can stick metal mason jar lids under that and raise it up so you run your plates.


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Invisibledna24
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373455 - 06/24/23 10:03 PM (1 year, 6 months ago)

like this


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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: dna24]
    #28373459 - 06/24/23 10:06 PM (1 year, 6 months ago)

Yeah, that's what I've got. Mine's a Presto too.


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OfflineBajazly
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Re: Pressure cooking fail [Re: dna24]
    #28373461 - 06/24/23 10:12 PM (1 year, 6 months ago)

Stack a couple jar bands or some smashed down aluminum can under the trivet that came with the PC and all will be fine. Use 3 stacks/cans for easy balance.  You could also use 3 half pint jars to space it up. This was a rookie mistake.

Edit: You could probably put the trivet on top of the steamer basket and be fine too.

Edited by Bajazly (06/24/23 11:33 PM)

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373463 - 06/24/23 10:14 PM (1 year, 6 months ago)

At very least wrap them in foil. I'd invest in one of these:

https://www.fishersci.ca/shop/products/fisherbrand-sterilizer-box-petri-dishes/03460#

Sterilize them while empty, then pour.

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InvisiblePickle Rick
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373499 - 06/24/23 11:05 PM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
At very least wrap them in foil. I'd invest in one of these:

https://www.fishersci.ca/shop/products/fisherbrand-sterilizer-box-petri-dishes/03460#

Sterilize them while empty, then pour.




these are awesome, I got some cheap ones from aliexpress. they also free'd up space in my pc, as I dry oven sterilize these days


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Offlinesonson
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Re: Pressure cooking fail [Re: Pickle Rick]
    #28373535 - 06/24/23 11:55 PM (1 year, 6 months ago)

I use five empty cans(from some canned seafood :smile: )  to keep the ideal rack height.



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Edited by sonson (06/25/23 12:05 AM)

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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373797 - 06/25/23 07:36 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
Sterilize them while empty, then pour.



Why not sterilize them after pouring? Then there's no chance of contamination.

Also $130 for some sheet metal is exactly what I expect from science suppliers. Why do they do it?


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Edited by Arctic_Fox (06/25/23 07:38 AM)

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373805 - 06/25/23 07:42 AM (1 year, 6 months ago)

You can find them cheaper, the link was just for example. Doing "no pour" glass petris is possible but seems like a pita. Pouring plates is easy af, ivenever contaminated a petri dish due to pouring, ever.

Dry sterilization seems the way to go, like pickle rick said.

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OfflineArctic_Fox
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373811 - 06/25/23 07:49 AM (1 year, 6 months ago)

I'm just thinking that if I have to sterilize the agar anyway, why not do it after pouring.

And you can sterilize in an oven? Why do people use pressure cookers then?


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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: Arctic_Fox]
    #28373814 - 06/25/23 07:52 AM (1 year, 6 months ago)

Only the glass petris, you can't use the oven for anything else. Dry heat is ineffective. Dry oven heat is sufficient for the nonporous glass petris, the oven will greatly reduce the risk of condensation.  Sterilizing "no-pour" petris in the pc means you'll have to be very careful not to disturb anything while the agar sets in the pc. You'll likely have very annoying levels of condensation as a result.

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap] * 1
    #28373828 - 06/25/23 08:15 AM (1 year, 6 months ago)

I do no pour with PP5 petris. Condensation is really the biggest problem you can run into but I've taken to using a couple of empty plates on the top of each stack for the pc cycle then just leaving the sterilized plates unwrapped in my culture drawer until I use them. They usually dry up in a day or two. Haven't had any contam issues either leaving them unwrapped.

Sterilizing agar is also usually the last thing I do for the day and after I turn the heat off I just go to bed everything is set by the morning. I do wrap the stacks in foil for the pressure cooker cycle just so they are easy to handle. Foil gets carefully removed after and they get gently placed in the drawer.

If you're gonna leave your plates unwrapped don't go waving them around and shit.


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373830 - 06/25/23 08:19 AM (1 year, 6 months ago)

No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373832 - 06/25/23 08:26 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.



Mine are 100x15mm vented petris


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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InvisibleStipe-n CapMDiscord
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Re: Pressure cooking fail [Re: SirPsycho]
    #28373843 - 06/25/23 08:33 AM (1 year, 6 months ago)

Pp5 petris exist? :mindblown:

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OfflineSirPsycho
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Re: Pressure cooking fail [Re: Stipe-n Cap]
    #28373848 - 06/25/23 08:38 AM (1 year, 6 months ago)

Yep, not as clear as glass but overall pretty great. Although someone did show me some very clear pp5 petris recently

Upside down:


Right side up:


Vent foot inside edge of lid:


They do also get scratched up by scalpels and other tools.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicornsβ€”We’re Building Quality (Olympus Myco Grow Bags)


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