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Arctic_Fox
Mr. Furious


Registered: 03/13/23
Posts: 460
Loc: Ontario
Last seen: 4 days, 7 hours
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Pressure cooking fail
#28373344 - 06/24/23 08:06 PM (1 year, 6 months ago) |
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The thing that I was using to elevate the petri dishes vibrated into a hole and fell over and ruined the batch.
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Yuggoth
Mi-Go Cultivator



Registered: 03/04/23
Posts: 558
Loc: Lost Carcosa
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Re: Pressure cooking fail [Re: Arctic_Fox] 1
#28373352 - 06/24/23 08:15 PM (1 year, 6 months ago) |
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I have the same wobbly steamer basket. This was $12 well spent.
https://www.amazon.com/dp/B078KVXQYC
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dna24
Darth Randal



Registered: 04/19/22
Posts: 511
Loc: Savages
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Re: Pressure cooking fail [Re: Arctic_Fox]
#28373413 - 06/24/23 09:19 PM (1 year, 6 months ago) |
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Quote:
Arctic_Fox said:

The thing that I was using to elevate the petri dishes vibrated into a hole and fell over and ruined the batch.

what is that thing? you cant just use jar lids? or the rack the pc comes with? ive done a double rack setup before by using the one from the instantpot
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Arctic_Fox
Mr. Furious


Registered: 03/13/23
Posts: 460
Loc: Ontario
Last seen: 4 days, 7 hours
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Re: Pressure cooking fail [Re: dna24]
#28373437 - 06/24/23 09:52 PM (1 year, 6 months ago) |
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It didn't come with a rack other than the one that keeps things a centimeter off the bottom.
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dna24
Darth Randal



Registered: 04/19/22
Posts: 511
Loc: Savages
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Re: Pressure cooking fail [Re: Arctic_Fox]
#28373448 - 06/24/23 09:59 PM (1 year, 6 months ago) |
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is it a rack or a metal type plate thing with holes in it? my presto has the plate type which ya isnt that high up but you can stick metal mason jar lids under that and raise it up so you run your plates.
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dna24
Darth Randal



Registered: 04/19/22
Posts: 511
Loc: Savages
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Re: Pressure cooking fail [Re: Arctic_Fox]
#28373455 - 06/24/23 10:03 PM (1 year, 6 months ago) |
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like this
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Arctic_Fox
Mr. Furious


Registered: 03/13/23
Posts: 460
Loc: Ontario
Last seen: 4 days, 7 hours
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Re: Pressure cooking fail [Re: dna24]
#28373459 - 06/24/23 10:06 PM (1 year, 6 months ago) |
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Yeah, that's what I've got. Mine's a Presto too.
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Bajazly
Stranger... Than You Think



Registered: 09/02/22
Posts: 832
Loc: BC, Mexico
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Re: Pressure cooking fail [Re: dna24]
#28373461 - 06/24/23 10:12 PM (1 year, 6 months ago) |
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Stack a couple jar bands or some smashed down aluminum can under the trivet that came with the PC and all will be fine. Use 3 stacks/cans for easy balance. You could also use 3 half pint jars to space it up. This was a rookie mistake.
Edit: You could probably put the trivet on top of the steamer basket and be fine too.
Edited by Bajazly (06/24/23 11:33 PM)
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,269
Loc: Canada
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Re: Pressure cooking fail [Re: Arctic_Fox]
#28373463 - 06/24/23 10:14 PM (1 year, 6 months ago) |
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At very least wrap them in foil. I'd invest in one of these:
https://www.fishersci.ca/shop/products/fisherbrand-sterilizer-box-petri-dishes/03460#
Sterilize them while empty, then pour.
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Pickle Rick
Gherkin



Registered: 07/24/22
Posts: 866
Loc: Jar
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Quote:
Stipe-n Cap said: At very least wrap them in foil. I'd invest in one of these:
https://www.fishersci.ca/shop/products/fisherbrand-sterilizer-box-petri-dishes/03460#
Sterilize them while empty, then pour.
these are awesome, I got some cheap ones from aliexpress. they also free'd up space in my pc, as I dry oven sterilize these days
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sonson
Monkey King


Registered: 01/01/23
Posts: 185
Loc: Asia or Oceania
Last seen: 3 months, 17 days
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I use five empty cans(from some canned seafood ) to keep the ideal rack height.
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Edited by sonson (06/25/23 12:05 AM)
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Arctic_Fox
Mr. Furious


Registered: 03/13/23
Posts: 460
Loc: Ontario
Last seen: 4 days, 7 hours
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Quote:
Stipe-n Cap said: Sterilize them while empty, then pour.
Why not sterilize them after pouring? Then there's no chance of contamination.
Also $130 for some sheet metal is exactly what I expect from science suppliers. Why do they do it?
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Edited by Arctic_Fox (06/25/23 07:38 AM)
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,269
Loc: Canada
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Re: Pressure cooking fail [Re: Arctic_Fox]
#28373805 - 06/25/23 07:42 AM (1 year, 6 months ago) |
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You can find them cheaper, the link was just for example. Doing "no pour" glass petris is possible but seems like a pita. Pouring plates is easy af, ivenever contaminated a petri dish due to pouring, ever.
Dry sterilization seems the way to go, like pickle rick said.
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Arctic_Fox
Mr. Furious


Registered: 03/13/23
Posts: 460
Loc: Ontario
Last seen: 4 days, 7 hours
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I'm just thinking that if I have to sterilize the agar anyway, why not do it after pouring.
And you can sterilize in an oven? Why do people use pressure cookers then?
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,269
Loc: Canada
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Re: Pressure cooking fail [Re: Arctic_Fox]
#28373814 - 06/25/23 07:52 AM (1 year, 6 months ago) |
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Only the glass petris, you can't use the oven for anything else. Dry heat is ineffective. Dry oven heat is sufficient for the nonporous glass petris, the oven will greatly reduce the risk of condensation. Sterilizing "no-pour" petris in the pc means you'll have to be very careful not to disturb anything while the agar sets in the pc. You'll likely have very annoying levels of condensation as a result.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
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I do no pour with PP5 petris. Condensation is really the biggest problem you can run into but I've taken to using a couple of empty plates on the top of each stack for the pc cycle then just leaving the sterilized plates unwrapped in my culture drawer until I use them. They usually dry up in a day or two. Haven't had any contam issues either leaving them unwrapped.
Sterilizing agar is also usually the last thing I do for the day and after I turn the heat off I just go to bed everything is set by the morning. I do wrap the stacks in foil for the pressure cooker cycle just so they are easy to handle. Foil gets carefully removed after and they get gently placed in the drawer.
If you're gonna leave your plates unwrapped don't go waving them around and shit.
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,269
Loc: Canada
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Re: Pressure cooking fail [Re: SirPsycho]
#28373830 - 06/25/23 08:19 AM (1 year, 6 months ago) |
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No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 7,340
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Quote:
Stipe-n Cap said: No pour makes perfect sense when using pp5 containers. It seems silly with glass petris, but that's my own personal bias, I suppose. They seem too shallow, mostly. The potential for spillage while setting, and massive condensation is high.
Mine are 100x15mm vented petris
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
Posts: 8,269
Loc: Canada
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Re: Pressure cooking fail [Re: SirPsycho]
#28373843 - 06/25/23 08:33 AM (1 year, 6 months ago) |
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Pp5 petris exist?
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
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Yep, not as clear as glass but overall pretty great. Although someone did show me some very clear pp5 petris recently
Upside down:
Right side up:
Vent foot inside edge of lid:

They do also get scratched up by scalpels and other tools.
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Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens
Balance in life is like running on ice.
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