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OfflineiLoveramen
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Spawn bags keep getting contaminated with black.
    #28372866 - 06/24/23 02:40 PM (1 year, 6 months ago)





I have been having a lot of problems with my grain after switching over to milo and millet.
I soak my grain for 18 hours and dry it. Then load it into an autoclave for 2 hours
I then do a G2G transfer.
Currently I have about a 30% success rate. I don’t know what is happening. They are all becoming contaminated with this black looking mold. I work in front of a laminar flow filter, and practice sterile procedures.

Could this possibly be due to not letting the grain dry out properly? I feel the grain could have been a little more dry but it was not soaking wet after placing in auroclave. I let air dry for about an hour.

Any input into why every bag is being contaminated black would be very helpful.

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OfflineLand TroutM
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Re: Spawn bags keep getting contaminated with black. [Re: iLoveramen] * 1
    #28372892 - 06/24/23 03:03 PM (1 year, 6 months ago)

Molds are easily killed by heat, and moisture doesn’t cause contamination, contaminates do. most likely that it’s getting In while you are doing your transfers.

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InvisibleStipe-n CapMDiscord
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Re: Spawn bags keep getting contaminated with black. [Re: Land Trout]
    #28372938 - 06/24/23 03:35 PM (1 year, 6 months ago)

:whathesaid:

G2g with bags is a bit trickier than with jars alone.

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InvisibleMycolorado
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Re: Spawn bags keep getting contaminated with black. [Re: Stipe-n Cap]
    #28373025 - 06/24/23 04:24 PM (1 year, 6 months ago)

The grains being transferred could also be contaminated.

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InvisibleStipe-n CapMDiscord
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Re: Spawn bags keep getting contaminated with black. [Re: Mycolorado]
    #28373044 - 06/24/23 04:38 PM (1 year, 6 months ago)

Yeah, that mold seems to be far too extensive for a simple hand hover mishap.

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OfflineMadSeasonStudent
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Re: Spawn bags keep getting contaminated with black. [Re: iLoveramen]
    #28373133 - 06/24/23 05:40 PM (1 year, 6 months ago)

Whatever your transferring to grain jar is contaminated.
Do you have a picture of the jars your using for spawn?


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OfflineiLoveramen
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Re: Spawn bags keep getting contaminated with black. [Re: Stipe-n Cap]
    #28373264 - 06/24/23 07:15 PM (1 year, 6 months ago)

When I did the G2G transfer, the bags were was fully colonized without any contamination.
Could it be I need to sterilize the bags longer than 2 hours in the autoclave?

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InvisibleStipe-n CapMDiscord
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Re: Spawn bags keep getting contaminated with black. [Re: iLoveramen]
    #28373276 - 06/24/23 07:20 PM (1 year, 6 months ago)

Depending on the size, most standard spawn bags hold 4+ lbs. 3.5-4 hours for that size. As mentioned before, molds die quickly and at relatively low temps, so even at 2 hours all mold should be gone.

Culprits could be:

The culture; spawn you used for expansion, sloppy technique, pinholes in bag, or faulty seal/filter.

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OfflineLand TroutM
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Re: Spawn bags keep getting contaminated with black. [Re: iLoveramen] * 1
    #28373305 - 06/24/23 07:38 PM (1 year, 6 months ago)

How were the masters inoculated?
Not that you can’t use bags for masters, they do seem to be greater vectors to contaminate the receiving bags Vs. jars.

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OfflineiLoveramen
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Re: Spawn bags keep getting contaminated with black. [Re: Land Trout]
    #28373383 - 06/24/23 08:53 PM (1 year, 6 months ago)

Masters were inoculated with agars.
My plan is to introduce some bags I just sterilizer using the same method into the same environment. This way I can check to see if it is my bags or sterilizing technique.
I need to start crossing off the list to see where I went wrong.
I’m very frustrated right now.

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