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Offlinedreamcows
Stranger
Registered: 06/06/23
Posts: 3
Last seen: 1 year, 4 months
pf tek germination
    #28366579 - 06/20/23 03:40 AM (1 year, 6 months ago)

Hey

New to this and started off with some BRF cakes and I've got no signs of germination just wondering if I've done something wrong as i did the following:

240ml (1/2 pint) wide mouth mason jars
2 part verm
1 part water
1 part brown rice flour
made sure verm was wet but not dripping

put a 1/2 inch layer of verm ontop of the mix in the jar

I don't own a PC but a multicooker with a canning feature and let it go for 120 mins and then waited over night (8+ hours) to cool down before taking them out

inoculated in a SAB, made sure everything was as clean as possible before going in, iso sprayed and let dry the inside of the SAB and then wet down the inside of the SAB with bleach water mix and had a damp bleach water towel laid down inside as a clean surface

shook the syringe before inoculating and flame sterilised and 70% iso wiped needles before each jar



and the jars are currently like below

sorry the pictures aren't that interesting

jar 1/2




jar 3/4




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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,801
Loc: Germany
Trusted Cultivator
Re: pf tek germination [Re: dreamcows]
    #28366608 - 06/20/23 04:35 AM (1 year, 6 months ago)

Quote:

dreamcows said:
shook the syringe before inoculating and flame sterilised and 70% iso wiped needles before each jar




Please forget about wiping the needle with iso
as you want to sterilize it, not sanitize.
Hope you wiped first, then flamed.
The other way is never recommended.
Just flame.

The rest sounds ok,
but when did you inoculate?
Depending on the age of spores,
it can take some time to see signs of germination.

          :cookiemonster:


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Offlinedreamcows
Stranger
Registered: 06/06/23
Posts: 3
Last seen: 1 year, 4 months
Re: pf tek germination [Re: Goatrider]
    #28366614 - 06/20/23 04:43 AM (1 year, 6 months ago)

yes I did flame then iso. I'll rectify that next time thank you for pointing that out. I assume I need to wait for the needle to cool before injecting because of the heat damaging the spores(?)

today will be going on 6 days since I've inoculated them

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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,801
Loc: Germany
Trusted Cultivator
Re: pf tek germination [Re: dreamcows] * 1
    #28366617 - 06/20/23 04:46 AM (1 year, 6 months ago)

You can squeeze the barrel gently to release a little drop,
or you can wait ~20-30 seconds to let the needle cool down.
6 days isn´t that much, just be patient.
The hardest part :biggrin:

      :cookiemonster:


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Offlinedreamcows
Stranger
Registered: 06/06/23
Posts: 3
Last seen: 1 year, 4 months
Re: pf tek germination [Re: Goatrider]
    #28366618 - 06/20/23 04:51 AM (1 year, 6 months ago)

easy mate cheers for the information, I wasn't sure if I needed to change anything next time considering the lack of any sign of germination :smile:

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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,801
Loc: Germany
Trusted Cultivator
Re: pf tek germination [Re: dreamcows]
    #28366622 - 06/20/23 04:58 AM (1 year, 6 months ago)

It´s just important you understand the meaning of sterilizing and sanitizing.
As you sterilized your jars,
you never want to put something in that isn´t sterilized either.
So with finally wiping, possibly something landed on the needle
that just got sanitized, but not killed.
It will enter the jar as a sleeper,
or worst case starts work immediately.
You may be lucky it worked though,
as you don´t see signs of unwanted growth.
So kill everything on the needle with flaming,
and repeat this for each other jar.
This weekend you should see germination :wink:

          :cookiemonster:


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