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Offlineprawnmunch3r
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Revival of B+ LQ, jellified at RTC for 3 years, revived on agar.
    #28360671 - 06/15/23 10:43 AM (7 months, 9 days ago)

I collected and made this LQ from a B+ Variety which I fruited in 2020. I Innocued Early in the year (Probs march/april), into a 4% normal honey/water mix. It quickly colonized and I kinda neglected it till earlier this week. In the 3 years, the temperatures fluctuated between 9c and 36c with varying levels of ambient humidity.

In the below pic, I'm holding the jar angled 45deg to show the LQ has been jellified.



I agitated it till some water seperated from it, then I needled up approx 1ml of LQ. Putting it on LME agar and incubating it at 27c reveals mycelium like growth on agar (within 3 days).





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Offlineprawnmunch3r
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Re: Revival of B+ LQ, jellified at RTC for 3 years, revived on agar. [Re: prawnmunch3r]
    #28361700 - 06/16/23 04:40 AM (7 months, 8 days ago)

The reason I posted this is to propose the idea that LQ is more resilient than given credit, given that I was able to obtain viable growth on agar after having it been dehydrated and jellyfied over 3 years, unrefrigerated, undergoing large temperature swings.


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OfflinePho
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Re: Revival of B+ LQ, jellified at RTC for 3 years, revived on agar. [Re: prawnmunch3r]
    #28362005 - 06/16/23 11:41 AM (7 months, 8 days ago)

This is pretty interesting, I have some LC that's over a year old that's been in the closet. I'm hoping it's still good, and this post gives me hope haha


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Offlinerifampicin
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Re: Revival of B+ LQ, jellified at RTC for 3 years, revived on agar. [Re: Pho]
    #28362101 - 06/16/23 12:40 PM (7 months, 8 days ago)

Good to know! I was exploring some long term storage and LC isn't that bad then (yeah I know agar slants are better still)


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Offlinetrippleblack
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Re: Revival of B+ LQ, jellified at RTC for 3 years, revived on agar. [Re: rifampicin]
    #28362182 - 06/16/23 02:12 PM (7 months, 8 days ago)

what you hope is that it was quasi freeze dried; but that is usually done under negative pressure without oxygen. 

coming back to life on agar is kinda cool.. a perfect fruiting culture  with no genetic changes is the most important part; let us know how it fruit.


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Offlineprawnmunch3r
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Re: Revival of B+ LQ, jellified at RTC for 3 years, revived on agar. [Re: trippleblack]
    #28365202 - 06/19/23 08:17 AM (7 months, 5 days ago)

I plan to, Im growing it out a bit more on agar, then Im going to isolate some, agar it again, then x-fer it to grain and see if it fruits.


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OfflineShadowfungus
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Re: Revival of B+ LQ, jellified at RTC for 3 years, revived on agar. [Re: prawnmunch3r]
    #28435829 - 08/16/23 08:27 AM (5 months, 9 days ago)

This summer I took a syringe of B+ LC that had been in a closet since 2018 and found the mycelium still grew on agar. Given that, I tried inoculating a small PF jar and the growth was slow, thin, and looked weak, but it colonized and fruited. I'm optimistic your experiments will be fruitful as well.


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