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OfflineFeelFighters
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Registered: 06/06/23
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28360829 - 06/15/23 01:06 PM (1 year, 6 months ago)

What's easier/simpler for someone who has never worked with Agar before? Pour or no-pour? I read the massive thread on why No-pour sucks by Rua (which I don't think it does) but for someone who has never done it, I feel fairly confident in doing pour but it seems a little more involved. Seems as though you prefer poured. Any insight?


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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: FeelFighters]
    #28360855 - 06/15/23 01:23 PM (1 year, 6 months ago)

I've never used no-pour agar, petri dishes are super simple. Either of them are a good choice, both are easy af. Petri dishes are more of an investment, financial resources would be the determining factor.

I've made a number of comments in the thread you mentioned, the issue was user error in the end, I believe.

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OfflineFeelFighters
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap] * 1
    #28360957 - 06/15/23 02:08 PM (1 year, 6 months ago)

Thank you! And yes it seemed user error did him in, though it was as if he was in denial the whole time... I've been trying to read as many threads as I can to kind of get an idea of what I'm getting into with Agar. I think I'll do the pour method, any borosilicate media bottle with gl45 cap will work for the pc correct? And a sleeve of 40 petri dished 90mmx15 seems to be the most common? Thanks again!


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Invisiblealtford78
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Registered: 05/09/23
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Re: Let’s talk Agar Recipes [Re: FeelFighters]
    #28360970 - 06/15/23 02:13 PM (1 year, 6 months ago)

40 sleeve? that's not going to fit in any standard sab


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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: FeelFighters] * 2
    #28360993 - 06/15/23 02:27 PM (1 year, 6 months ago)

I buy boxes of 500. 100mm x 15:

https://www.coleparmer.ca/i/celltreat-scientific-products-229694-slideable-sterile-petri-dishes-100-x-15-mm-500-cs/0193228

And use 500ml Pyrex media bottles, orange cap, 4 pack:

Pyrex 1399-500CN-M Slim line Media Bottle Easy Pour Corning 500ML Ca, Glass (Pack of 4) https://a.co/d/iV6rVMo

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OfflineFeelFighters
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Re: Let’s talk Agar Recipes [Re: altford78]
    #28361038 - 06/15/23 02:56 PM (1 year, 6 months ago)

It's a pretty big SAB, what's the typical number in a sleeve when pouring? Since it's my first time I don't want to go balls to wall per se, but money also is not an issue. SnC buys in box of 500, not sure how many per sleeve, but just starting out idk if I want THAT many. Thank you!


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OfflineFeelFighters
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28361044 - 06/15/23 02:57 PM (1 year, 6 months ago)

Thank you for the help! Cheers!  :toast:


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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: FeelFighters]
    #28361046 - 06/15/23 03:00 PM (1 year, 6 months ago)

Quote:

FeelFighters said:
SnC buys in box of 500, not sure how many per sleeve




The Celltreat are 20 plates/sleeve. Other brands usually come with 25/sleeve.

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OfflineFeelFighters
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap] * 2
    #28361084 - 06/15/23 03:34 PM (1 year, 6 months ago)

Just found some celltreat on Amazon $90 for 500, and a pack of 4 slimline pyrex media bottles w/ orange caps $65. Ordered. LME is on the way and I picked up Telephone agar last night. Next week I should be doing my own pours. Whats your opinion on the premade agar plates? They seem pretty expensive for how little you get, and perhaps a little sketchy coming in and not being contamd. Thanks again for the help!!


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How would you feel if you hadn't eaten breakfast?

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Invisiblemushhead
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Re: Let’s talk Agar Recipes [Re: FeelFighters] * 1
    #28361144 - 06/15/23 04:04 PM (1 year, 6 months ago)

Don't ever buy premade agar plates or grain spawn.
Always make your own and sterilize your own, this way you can make sure it's usable


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OfflineFeelFighters
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Re: Let’s talk Agar Recipes [Re: mushhead]
    #28361163 - 06/15/23 04:14 PM (1 year, 6 months ago)

:takingnotes: Noted. Thank you.


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Offlinesprocket
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Re: Let’s talk Agar Recipes [Re: FeelFighters]
    #28361210 - 06/15/23 04:39 PM (1 year, 6 months ago)

Would using 1.6-1.8% nutrients help with cleaning up germ plates


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: sprocket]
    #28361216 - 06/15/23 04:46 PM (1 year, 6 months ago)

Use a loop or cell spreader. Properly applying spores to agar will significantly increase your chances of separation.

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Offlinesprocket
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28361223 - 06/15/23 04:51 PM (1 year, 6 months ago)

I meant for taking transfers from a germ plate or from a plate with some contamination. I know I should take my transfer far from Amy obvious contamination just seeking an expert opinion on low nutrient agar. I've been battling some contaminated jars and want to get the cleanest mycelium possible to eliminate it as a source of contamination. I will also be PCing my jars for a full 2 hours from now on.


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

Edited by sprocket (06/15/23 04:51 PM)

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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: sprocket] * 2
    #28361230 - 06/15/23 04:57 PM (1 year, 6 months ago)

In all fairness to the OP, let's move that line of questioning to the gen discussion thread, or the agar envy thread so as not to high jack this thread.

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Offlinesprocket
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28361234 - 06/15/23 05:00 PM (1 year, 6 months ago)

Ok, sorry for that.


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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OfflineTonyCanoli
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Registered: 03/16/23
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Re: Let’s talk Agar Recipes [Re: sprocket] * 1
    #28361729 - 06/16/23 05:39 AM (1 year, 6 months ago)

Heck yeah guys this conversation went way further than I ever imagined. Love this place.


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Exploring the Myceliverse sharing Spore Stories

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Invisibledna24
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Re: Let’s talk Agar Recipes [Re: mushhead] * 1
    #28361844 - 06/16/23 08:25 AM (1 year, 6 months ago)

Quote:

mushhead said:
Honestly I adopted the way Stipe does his agar.
It has produced nothing but good results for me and I was having a rough time because of a few different issues.
Pro-tip: Don't use five-six year old LME.
https://www.shroomery.org/forums/showflat.php/Number/28354611#28354611





shit i use 10yr old lme and its perfectly fine, its how its held that matters


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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: dna24] * 1
    #28361886 - 06/16/23 09:20 AM (1 year, 6 months ago)

:whathesaid:

Storing your powders in air tight containers will keep them indefinitely. I keep my malt in a glass, swing-top container.

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OfflineWhatEyeFind
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Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 1
    #28361918 - 06/16/23 10:09 AM (1 year, 6 months ago)

I am on my fifth batch of agar, I had many failures, and I have learned five ways to NOT make agar plate and cup. Allow me to share.

I just got my agar to show life starting from a few spores in just three days. Finally. It seems that I have found two ways that work, and are crystal clear.

I boiled a scrubbed red potato (not washed, just lightly scrubbed with brush under water, no soap), about 110-135g (sliced) boiled in 500ml 'purified' water (a plastic 20+/- oz bottle is 500 ml) for 15 minutes and then I fished out the slices with a fork. Allow the water to cool and top up to 500 ml with purified water to compensate for evaporation during cooking.

- Lightly salt and eat the potato slices while waiting to cool to room tempº -

Added 8-10g agar to cooled water and stir. (** I should say, if you really want it crystal clear, use a coffee filter first, before agar, to remove any remaining red skin flits or starch cloudiness) mine is clear enough for me.
Agar mixes into cool water better than into hot liquid. (Ask grandma about mixing gravy with flour water or corn starch, she'll tell you to mix it cool)
Cook (slow boil) for 10 minutes and then pour into your media bottle. I recycled a few glass kombucha bottles with a long neck as I couldn't afford fancy liquor like Roger Rabbit.
PC for 20 minutes @ 15psig AFTER the recommended 15 minutes of PC venting. Turn off stove and let cool (under pressure) at very least, until the safety plunger drops, but better to cool for about two hours. Go watch Alice in Wonderland on DVD? -and then allow it to cool until you can barely stand to handle it.
Using a flow hood or SAB, follow stack pouring petri plate techniques and don't forget to twist the bottle like a sommelier serving fancy wine at the end of each plate fill. I also pour fill about a dozen 2oz Redi cups.

My first agar attempt (and first agar failure) was PGT's 3-2-1 and I think I learned that my 2 year old potato flakes AND / OR the Karo Corn (fresh) syrup was the break point on that. BUT I did learn my PC, and I got practice pouring in my SAB... I was later advised that it was too nutrient rich and had I just used potato flakes and not used sugar (Karo), I might have had better success.

Attempts 2-5 were better, nice, clear, 2% nutrient... I learned from my previous mistakes BUT that shit couldn't culture a sneeze.

I now believe that perhaps my Walmart GEL food coloring, that the sulfites and preservative may have been my problem.

I merely dipped a bamboo toothpick and swirled to tint but nothing would grow. They sure were pretty plates though...




* I am just getting started so take my advice with a pinch of... 'nutritional yeast'?

Edited by WhatEyeFind (06/16/23 11:02 AM)

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