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OfflineTonyCanoli
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Registered: 03/16/23
Posts: 218
Last seen: 1 year, 6 months
Let’s talk Agar Recipes * 1
    #28360655 - 06/15/23 10:21 AM (1 year, 6 months ago)

I know there are likely multiple threads for all the different agar recipes and they likely contain a ton of useful information.

I’m basically trying to compile all that information with this thread.

Share your agar recipes and process! Let’s hear it!

I’m blown away by how clear some of you guys can get your plates and I’d like to know what some of the pros are using in real time.

I use the PGT Agar recipe:
3 parts potato flakes - 12 grams
2 parts agar agar - 8 grams
1 part corn syrup - 4 grams
Water - 400 ml - 2 cups


And I can’t imagine I’ll ever get clear agar using something like potato flakes .

So let’s talk about it!

The what and why, let’s go bros! I’m due for a new batch of agar so I’ll start trying some recipes!


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Exploring the Myceliverse sharing Spore Stories

Edited by TonyCanoli (06/15/23 10:22 AM)

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Invisiblemushhead
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Registered: 08/22/14
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Loc: Dimension G-452
Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 1
    #28360658 - 06/15/23 10:27 AM (1 year, 6 months ago)

Best recipe I can recommend is 10-15G LME, 8-10g agar-agar, and Filtered or Distilled water.
PC at 45min. Trust I have gone through about 20 or so different recipes.


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OfflineTonyCanoli
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Registered: 03/16/23
Posts: 218
Last seen: 1 year, 6 months
Re: Let’s talk Agar Recipes [Re: mushhead]
    #28360661 - 06/15/23 10:30 AM (1 year, 6 months ago)

So do you bring the ingredients to a boil together and then PC or do you just mix and then PC and let the PC cook?


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Invisiblemushhead
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Registered: 08/22/14
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Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 1
    #28360665 - 06/15/23 10:32 AM (1 year, 6 months ago)

Honestly I adopted the way Stipe does his agar.
It has produced nothing but good results for me and I was having a rough time because of a few different issues.
Pro-tip: Don't use five-six year old LME.
https://www.shroomery.org/forums/showflat.php/Number/28354611#28354611


--------------------
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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 4
    #28360670 - 06/15/23 10:40 AM (1 year, 6 months ago)

Agar doesn't require loads of nutrients. Click the why agar link in my signature, it's pretty comprehensive.

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OfflineTonyCanoli
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Registered: 03/16/23
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Last seen: 1 year, 6 months
Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28360676 - 06/15/23 10:50 AM (1 year, 6 months ago)

I can’t see your signature. Am I missing something? I’m relatively stupid so pardon me lol.


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OfflineTonyCanoli
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Registered: 03/16/23
Posts: 218
Last seen: 1 year, 6 months
Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28360677 - 06/15/23 10:52 AM (1 year, 6 months ago)

Quote:

mushhead said:
Honestly I adopted the way Stipe does his agar.
It has produced nothing but good results for me and I was having a rough time because of a few different issues.
Pro-tip: Don't use five-six year old LME.
https://www.shroomery.org/forums/showflat.php/Number/28354611#28354611



Quote:

Stipe-n Cap said:
Agar doesn't require loads of nutrients. Click the why agar link in my signature, it's pretty comprehensive.




Oh ok just read it. So literally just ingredient in to watching temp water. Give it the shake weight treatment. PC that sucka for a while, cool, pour? Would i add food coloring with the other ingredients if I wanted to color it?


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Exploring the Myceliverse sharing Spore Stories

Edited by TonyCanoli (06/15/23 10:59 AM)

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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 3
    #28360699 - 06/15/23 11:18 AM (1 year, 6 months ago)

Malt and agar dumped into room temp distilled h2o, then pc for 45 mins @ 15psi.

You can add food coloring or other ammendments to taste, however I'd recommend starting with the the simple recipe first; as you gain experience, tinker as required.

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Invisiblemushhead
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap] * 2
    #28360705 - 06/15/23 11:26 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
Malt and agar dumped into room temp distilled h2o, then pc for 45 mins @ 15psi.

You can add food coloring or other ammendments to taste, however I'd recommend starting with the the simple recipe first; as you gain experience, tinker as required.



:whathesaid::cheers:

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OfflineTonyCanoli
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Registered: 03/16/23
Posts: 218
Last seen: 1 year, 6 months
Re: Let’s talk Agar Recipes [Re: mushhead]
    #28360707 - 06/15/23 11:32 AM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:
Malt and agar dumped into room temp distilled h2o, then pc for 45 mins @ 15psi.

You can add food coloring or other ammendments to taste, however I'd recommend starting with the the simple recipe first; as you gain experience, tinker as required.



Quote:

mushhead said:
Quote:

Stipe-n Cap said:
Malt and agar dumped into room temp distilled h2o, then pc for 45 mins @ 15psi.

You can add food coloring or other ammendments to taste, however I'd recommend starting with the the simple recipe first; as you gain experience, tinker as required.



:whathesaid::cheers:




Heck yeah dudes thank you. Just bought some LME and some more plates right now. I’ll be trying this out on the 18th!

Can we break down the recipe though?
So, 10-15G LME, 8-10g agar-agar, how much water for let’s say 10 & 8 LME & Agar


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Exploring the Myceliverse sharing Spore Stories

Edited by TonyCanoli (06/15/23 11:33 AM)

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InvisibleMycolorado
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Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 2
    #28360716 - 06/15/23 11:41 AM (1 year, 6 months ago)

2% nutes and agar of final media volume.

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Invisiblealtford78
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Posts: 890
Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 2
    #28360719 - 06/15/23 11:41 AM (1 year, 6 months ago)

You'd be better off going for percentage rather than a gram based recipe how you are doing it. Your life will be way easier. Like I see "recipes" with grams and I just zone out.

I asked Stipe and he does 2% AA/ 2% LME for all his plates. It's all in his thread here https://www.shroomery.org/forums/showflat.php/Number/28188696#28188696

Once you have the target concentration, you then get the grams needed for the total volume. Plug your numbers in here and you'll get the grams you need https://www.calculator.net/percent-calculator.html


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InvisibleMycolorado
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Re: Let’s talk Agar Recipes [Re: altford78] * 3
    #28360727 - 06/15/23 11:44 AM (1 year, 6 months ago)

You need a calculator for that? :crazy2:

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Invisiblealtford78
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Re: Let’s talk Agar Recipes [Re: Mycolorado] * 1
    #28360733 - 06/15/23 11:49 AM (1 year, 6 months ago)

Yes. :knowwhatnevermind:




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InvisibleMycolorado
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Re: Let’s talk Agar Recipes [Re: altford78]
    #28360738 - 06/15/23 11:52 AM (1 year, 6 months ago)

:rofl:

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OfflineHysteria
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Registered: 02/19/23
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28360752 - 06/15/23 11:57 AM (1 year, 6 months ago)

SnC I just switched to your brand of LME and method and did my first batch with it last night. I was using a different brand and Bod's method, but I never get that strong rope-like rhizo that other people get. It's usually much thinner and and doesn't look as strong. That, and I almost always get a fuzzy layer over the rhizo layer if I let it grow out. It's the same color as the rhizo and doesn't outpace it, and usually it's as uniform as "fuzzy" can be, so not necessarily mold or anything.
I want to see if making this switch gives me better-looking plates. Thank you for sharing, as always.

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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: TonyCanoli] * 3
    #28360756 - 06/15/23 11:59 AM (1 year, 6 months ago)

Quote:

TonyCanoli said:
Can we break down the recipe though?
So, 10-15G LME, 8-10g agar-agar, how much water for let’s say 10 & 8 LME & Agar




Every ml of h2o weights 1 gram. 1 kilo/ 1 Liter = 1000 grams, it's easy to remember :lol:

So, if you're using 400ml to pour a sleeve of 25: 2% of 400 = 8

If using 600ml: 12

1000ml: 20

2% agar/ 2% nutrients. 45 mins @ 15 psi, vent your pc :kingcrankey::lol:

Quote:

Hysteria said:
SnC I just switched to your brand of LME and method and did my first batch with it last night. I was using a different brand and Bod's method, but I never get that strong rope-like rhizo that other people get. It's usually much thinner and and doesn't look as strong. That, and I almost always get a fuzzy layer over the rhizo layer if I let it grow out. It's the same color as the rhizo and doesn't outpace it, and usually it's as uniform as "fuzzy" can be, so not necessarily mold or anything.
I want to see if making this switch gives me better-looking plates. Thank you for sharing, as always.





Keep in mind that whatever morphology you manage to achieve on agar will not be guaranteed to persist on grain. Rhizomorphs v tomentose is irrelevant.

Edit:

Whatever recipe you find, including the ones listed by either bod or myself are not our own intellectual property. We all borrow and adopt methods/recipes from the wider world of microbiology.

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OfflineHysteria
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap] * 1
    #28360767 - 06/15/23 12:07 PM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:


Keep in mind that whatever morphology you manage to achieve on agar will not be guaranteed to persist on grain. Rhizomorphs v tomentose is irrelevant.




Yes, I have read that, just don't have the experience to forsee what will happen on grain from the plate yet, so the more picture-perfect, the better for me, at this level.


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Invisiblealtford78
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Re: Let’s talk Agar Recipes [Re: Stipe-n Cap]
    #28360776 - 06/15/23 12:16 PM (1 year, 6 months ago)

Quote:

Stipe-n Cap said:


So, if you're using 400ml to pour a sleeve of 25: 2% of 400 = 8






Tf everyone pours entire sleeves with 400ml but I tap out at around 16-18 90mm petris. Probably need to pour hotter.


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InvisibleStipe-n CapMDiscord
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Re: Let’s talk Agar Recipes [Re: altford78] * 2
    #28360784 - 06/15/23 12:26 PM (1 year, 6 months ago)

Yeah, I pour pretty hot, just shy of removing my fingerprints :crazy2:

Otherwise I lose some to hardening at the end. I let the plates sit in flow for an hour or so before storing. The top plate usually has some condensation but the rest are fine. I always have blanks made well in advance of use, any condensation has evaporated by then. I find that if I wait until I need them I often won't feel like fucking with agar, so, preparing them when I feel like it definitely helps :lol:

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