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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61] 2
#28499184 - 10/10/23 06:01 PM (3 months, 16 days ago) |
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There's plenty of l-tryptophan already present in cereal grains. Raw wheat berries contain roughly 450mg of l-tryptophan/100g of wheat, for example.
The biogenesis of neurotransmitters and hormones such as serotonin or melatonin begins with l-tryptophan, along with the prized 4-phosphoryloxy-N,N-dimethyltryptamine.
On the otherhand, de novo biosynthesis from d-erythrose-4-phosphate and phosphoenol pyruvate requires 13 steps and 12 enzymes, with some unknown percentage of intact l-tryptophan potentially taken from the environment, but I'm not certain how much of the alkoloid content is produced de novo versus recruitment from the substrate, perhaps none, that is to say, other than the necessary amino acids.
I believe the chemistry is far too complicated to assume one generic precursor like l-tryptophan, added to what is essentially a swimming pool of already available l-tryptophan, would correlate directly to the production of more 4-phosphoryloxy-N,N-dimethyltryptamine.
To be certain, this would require some serious equipment and training to ascertain. With the abundance of l-tryptophan already present in cereal grains, I have serious doubts regarding supplentation, much like bringing sand to a very sandy beach.
I'm not certain but my gut tells me that melatonin is not along the correct pathway to result in 4-phosphoryloxy-N,N-dimethyltryptamine, feel free to correct me if I'm wrong....

But I don't appear to be.
This seems like the most reasonable position, in my humble opinion.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61] 4
#28499369 - 10/10/23 08:20 PM (3 months, 16 days ago) |
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Yeah, I have doubts that dmt just converts to psilocin when introduced to the sub. I have no idea why he'd think such a thing, but maybe I'm wrong. I'm not a a famous drug chemist, or any kind of chemist, and I doubt he was much of a mycologist.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61] 3
#28499669 - 10/11/23 06:03 AM (3 months, 15 days ago) |
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Psychoactive fungi are organisms which do not require many environmental resources, with the exception of water, to produce fruit and chemistry. The highest concentrations of these compounds are found in species growing in environments nearly void of resources other than decaying wood, grasses, and water.
I am inclined to believe that cereal grains by comparison are quite rich, and provides more than the organism can handle.

I'd be very surprised if it could be proven that supplementation beyond the addition of cereal grains would have any effect whatsoever, unless one could identify a gap in the provision supplied by any particular grain. Grains are rich in fats, proteins, amino acids, l-tryptophan, etc.
I would lean more in the direction of complex symbiosis with other organsms rather than direct feeding via supplementation.
Edited by Stipe-n Cap (10/12/23 07:51 AM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61] 1
#28501293 - 10/12/23 07:51 AM (3 months, 14 days ago) |
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Whoopsie doodle, "organisms". I write microorganism with inordinate frequency, my bad. Slip of the ol thumbs 
Edited for clarity.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: on_a_dventure] 3
#28549895 - 11/20/23 07:35 AM (2 months, 6 days ago) |
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Lol.
This is about boosting trypatamine content in fruit bodies by amending substrate with precursors like melatonin.
You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts.
Perhaps you can provide a few more details? I'd be interested to hear how you're achieving this.
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