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Offlineargenpills
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Registered: 06/14/23
Posts: 5
Last seen: 7 months, 1 hour
Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61]
    #28359336 - 06/14/23 08:54 AM (7 months, 10 days ago)

I will gladly replicate it to see if anything happens "by eye." But the reality is that this experiment from the beginning is destined to have no results, and let me explain why...

It would be good to carry out this experiment involving more people who are interested and knowledgeable or capable of conducting a chromatographic analysis of the control strain that we will be exposing to tryptophan. Initially, it would be good to have a control strain where we only administer one type of grain, perhaps oats... After a few weeks or months, when it bears fruit, we would have to dissect them at a constant temperature that does not exceed 50 degrees and micronize them to make a powder of that control strain. Then we would have to conduct a chromatographic analysis to determine the presence of tryptamines in that strain with that grain. Once we have the report on what that strain can metabolize with that type of grain we provided for nourishment, we will have an average value of what we can obtain with just the grain. Then we would have to repeat the entire procedure, but this time adding tryptophan.

It is a lengthy process that can take months or years. The only way to verify it is with scientific data because you won't notice anything at first glance.

To determine the potency of your psilocybin mushroom bread, you would need to harvest them, dissect them, micronize them, and perform chromatographic analysis in a laboratory or with people who are interested in these topics and dedicated to field research (there should be many qualified individuals).


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Offlineargenpills
Stranger
Registered: 06/14/23
Posts: 5
Last seen: 7 months, 1 hour
Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61]
    #28360950 - 06/15/23 02:06 PM (7 months, 9 days ago)

Friend, I've been researching and reading on the forum, and it seems like things are quite clear. While there aren't very specialized studies showing evidence with photos and chromatographic exams, in this article, you'll find quite a few answers.

Many of these experiments are conducted at "Oakland Hyphae" where they perform chromatographic exams on all participating strains. We should get in touch with those people to conduct tests like the ones mentioned above.

There are many scientists who do good science, but I believe that they, knowing the truth and holding the key to that knowledge, don't engage in debates on these topics. They simply do their thing. It could be everyone's secret.


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Offlineargenpills
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Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61]
    #28360973 - 06/15/23 02:15 PM (7 months, 9 days ago)



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Offlineargenpills
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Last seen: 7 months, 1 hour
Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: nektar61] * 1
    #28361891 - 06/16/23 09:24 AM (7 months, 8 days ago)

Regarding my account on this forum: I created my account specifically to comment on your thread. I'm from Argentina and I have been researching psychedelics for over 15 years (more focused on the synthetic side, particularly MDxx). It seems like not many people care about your thread, but it caught my attention, which is why I'm here, my friend.

I read through the thread, but I didn't quite understand your original post about the conclusions you had reached. English is not my native language, as I mentioned before. However, you explained your results in a simpler way here.

I am also conducting experiments on this subject, and I am confident that the questions that you and I have today already have answers. Someone has already conducted these experiments and knows well what happens. The thing is, those people are not hanging around internet forums, enlightening newcomers who want to play "Breaking Bad" with biological organisms (although it would be good if it happened, LOL).

Predicting what a biological organism will do is very difficult. We have to consider the starting point, such as starting with spores and how long ago those spores were taken from the mushrooms. If we start with agar on a plate or in a liquid culture, we have to make sure the mycelium is not "old" (mycelium ages and loses potency), so we need to regularly sexualize it with spores and allow it to create diploid mycelium.

The link I shared with you resolved many of these doubts for me but raised new ones that go beyond my current capacity for study. I don't think any of these guys here will join our conversation and explain to us how we can make our mushrooms better in a cheap way with a simple element...


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Offlineargenpills
Stranger
Registered: 06/14/23
Posts: 5
Last seen: 7 months, 1 hour
Re: Melatonin added to substrate, and also Tryptophan added to substrate [Re: x7x_x7x]
    #28373045 - 06/24/23 04:38 PM (7 months, 5 hours ago)

Brother... what the hell are you talking about? You wrote so much shit to contribute absolutely NOTHING to the conversation we were having with another user. If my comment hurt your LGBT feelings, I couldn't care less.

:nyan:


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