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Offlinemusshiegrwr
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Registered: 06/26/04
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Last seen: 13 years, 1 month
newbie PLEASE HELP ME!!!!
    #2834469 - 06/27/04 09:33 PM (13 years, 5 months ago)

ok everyone i have no problem growing pot plants but these musshies are much differnt ok first off i bought the spores for thailand-koh samui cubensis and costa rica psilocybe cubensis,  :mushroom2: i followed instructions perfectally stirilizing everything including my self lol now the recipe i used was 140 ml of verm to 40 ml of brown rice flour and water. when i put it in the jars it was packed down very lightly still leaving air inside. then i took a stiril pair of sizzors and cut up my spore print and slid the small pieces of print down into the cake not knowing at the time i had them under a 40 watt light bulb for the first 12 or so days and quickly realising my fault i changed this these were spored on may 10 and no change what so ever. now i have a second batch going i follwed the same recipe and did not put under the light and they were spored on the 5 of june ...what did i do wrong and is there any better ways....
please help me :confused: :sad:
and would it be worth my time putting these cakes that havn't done anything in a compost pile?


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OfflinePsilocybeingzz
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Registered: 12/15/02
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Re: newbie PLEASE HELP ME!!!! [Re: musshiegrwr]
    #2834481 - 06/27/04 09:38 PM (13 years, 5 months ago)

Why dint you inject th cake with sprores???
Never just slip some foil in a jar :nonono:
and when you opened it you didnt use a glovebox I assume, so chances are you are in for contams, sorry. :frown:


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Edited by Psilocybeingzz (06/27/04 09:39 PM)


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Offlineempath
I feel you

Registered: 03/03/04
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Re: newbie PLEASE HELP ME!!!! [Re: musshiegrwr]
    #2834482 - 06/27/04 09:39 PM (13 years, 5 months ago)

How do you know the scissors were sterile? I do not let anything touch my prints beside the foil its on and a syringe needle. What temp did you incubate your jars? You should have made syringes first

Seems like you need to do a little bit more reading before you try again. the FAQs on here are a great place to start.


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"It tastes like burning"-Ralph Wiggum


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Offlinemidway
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Re: newbie PLEASE HELP ME!!!! [Re: musshiegrwr]
    #2834483 - 06/27/04 09:39 PM (13 years, 5 months ago)

innoculating like that is asking for trouble contamination wise, as well as leaving most of the spores dormant from lack of moisture. Next time if you order spore prints, follow a tek for rehydrating the spores and getting them in a large guage syringe for injection innoculation.
Pays to read!
mid


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Offlinemusshiegrwr
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Re: newbie PLEASE HELP ME!!!! [Re: midway]
    #2834498 - 06/27/04 09:46 PM (13 years, 5 months ago)

i knew the scissors were stirile cause i stirilixed them first... i put the spores in at 75 deg room tmp


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OfflinePsilocybeingzz
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Re: newbie PLEASE HELP ME!!!! [Re: musshiegrwr]
    #2834509 - 06/27/04 09:49 PM (13 years, 5 months ago)

First of all thats a fruiting temp,other then that your spores were put in with no water,in an unclean enviroment....you dont just OPEN JARS without a GLOVEBOX :wink: its bad Mojo ( :thumbdown: contams)

Sterile scissors should be used for making prints etc, not for starting jars!

Try try TRY again :grin:


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Offlinecaimen
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Re: newbie PLEASE HELP ME!!!! [Re: Psilocybeingzz]
    #2834515 - 06/27/04 09:51 PM (13 years, 5 months ago)

Man im a noob too But if u read all the post these kick ass people make And the link they provide . the SEARCH works great. read the frist 2 post on this fourm


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InvisibleMagashM
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Re: newbie PLEASE HELP ME!!!! [Re: musshiegrwr]
    #2834557 - 06/27/04 10:09 PM (13 years, 5 months ago)

Way of putting in the spores is off, temps are off, 140 mil to 40 mil, 40 mil. You will get beter results if you try 120 mil verm, 60 brf, 60 water or 2 parts verm 1 part water, 1 part brf.

I know this belongs in the growlog forum but it seems more would see it here that can use it. That and I?m sure most have seen it already but maby somebody can use this to help them out.

Grow Log
Tek : PF
Strain : South American [SA]
Spores : Came from homemade prints. 36 total jars to be injected.
Substrate :Brown Rice Flour [BRF] and Vermiculite [Verm]
---------------------------------------------------------------------------------------------------
10:15 PM 3/4/03 : Project is started. Jar prep is just putting small holes in the lids for the spore syringe and then taping them closed until inoculation. Tape used is the standard black electrical tape. First batch of jars is mixed. The mix used is 2 parts verm, 1 part water, and 1 part brf. [ This mix is the max fruiting formula by PF.] Mix is put into 1/2 pint canning jars lightly, not packed in, and left it airy. Put substrate in until it reaches the threads of the jar, then add dry verm the rest of the way.
Jars are PCd at 10psi or 240f for a period of 45 minutes. Then jars were left to sit overnight to cool.



8:39 PM 3/5/2003 : First batch of jars injected. (24 jars)

10:04 AM 3/6/2003 : Incubator is running to low 77F. Going to turn it up today.



5:45 PM 3/6/2003 : Second batch of jars is in the PC will be injected tomorrow. Turned up incubator runs at 83F. 86F is the best and if the incubator is 83F and myc growing in the jars is suppose to create a little heat I figure this should work out well.

1:11 PM 3/7/2003 : Second batch of jars is done. (12 jars)

12:10 PM 3/9/2003 : Jars are doing good, no signs of contams



2:54 AM 3/19/2003 : Tape removed from the holes today and coffee filters put over the top of the jars for added protection. Holes were opened so the CO2 in the jars could escape. I gave 12 jars to a friend who kept fucking up. 24 jar?s about 75%done.



12:13 PM 3/22/2003 : Well it?s Saturday and the jars will probably be done by the 25th. So it was 36 jars. 12 were given to a friend, 24 done by Tuesday.

10:26 PM 3/23/2003 : 15 jars were done enough to put into dunk. I dunk and cold shock before birthing and after every flush. The other 9 jars have a spot the size of a pea that has to be covered so I?ll do those on the 25th.



7:17 PM 3/24/2003 : 15 jars put into the pod. Put in and put a layer of moist verm on top. With geolite I just put the cakes on it. With some other chambers I put the cake on a lid filled with verm. This is done to give the shrooms on the bottom and the top of the cake some added moisture.
The other 9 jars just finished so I?m going to give them a couple more days to make sure the middle of the cake is done.



4:57 PM 3/27/2003 : 5 More cakes birthed today, 4 left going to do them tomorrow or 3/29/2003

3:58 PM 3/29/2003 : Well I lost another cake. My wife?s little 1 cake pod made from a 2 liter and a small fish pump and a little geolite used up her last cake so I gave her a SA to grow. The last 3 are in the cold shock dunk.
So the total is 36 jars done all colonized with no contams. 12 given to a friend, 1 to the wife, 23 mine

12:08 PM 3/30/2003 : Last of jars put in

12:02 PM 3/31/2003 : The first 15 cakes are pinning nicely and some have shrooms coming from the bottom.



11:01 AM 4/3/2003 : Things look to be going well.



1:02 AM 4/4/2003 : Took the first flush from four of the cakes. More will be done later today.



5/8/2003 : Total 23 cakes 164 grams dried.

From what I?ve seen this needs to be up for a little bit.


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


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Offlinemusshiegrwr
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Registered: 06/26/04
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Re: newbie PLEASE HELP ME!!!! [Re: Magash]
    #2836022 - 06/28/04 12:11 PM (13 years, 5 months ago)

if 75 is fruiting temp what temp should i use too colonize the jars?
ty


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OfflineMinimumEfrt2
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Registered: 03/17/04
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Re: newbie PLEASE HELP ME!!!! [Re: musshiegrwr]
    #2836438 - 06/28/04 02:43 PM (13 years, 5 months ago)

like 85 degrees


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