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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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Well….I found two green splotches when I cut the liner- dark green and granular. Guess all this work and waiting is Fing wasted. Five months of trying. What do I do with this monotub? It is my only one. I cut the shit out, but I am betting it has sporulated. Any way to save this? Looks like another three months at least from scratch. Started all this nonsense with rye berries spawn bag and syringe. Can anything be recovered? Mycelium for tea? Pins? This sucks.
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phlanx
Experimentor


Registered: 06/11/05
Posts: 191
Loc: U.S.
Last seen: 4 months, 24 days
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Hey Hopeful,
Generally once trich shows up, it will end the tub. That said, if you don’t have anything else going, let it ride for as long as you are comfortable. Any fruit that grows will still be fine to eat, but you probably don’t want to let trich sporulate on anything you might eat, that would be real gross.
If you aren’t interested in trying to let your pins mature, then pick them now. Little baby shrooms can be potent little things.
Going forward, try to find a better method than spore to grain, it will just keep failing and be really expensive. Read all the teks in the stickied posts on the first page of mush cult and find one that looks good to you. If you want to use a spore syringe and want basically guaranteed fruit, do some pf cakes.
-Phlanx
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,340
Last seen: 10 months, 7 days
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Syringes are for agar or pf tek. Start with one of those and you’ll stop wasting your time.
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Way
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
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Re: Mono tub newbie [Re: Screwup] 1
#28306469 - 05/04/23 01:13 PM (1 year, 8 months ago) |
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+
That tub is fucked.
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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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Re: Mono tub newbie [Re: Way]
#28306585 - 05/04/23 02:37 PM (1 year, 8 months ago) |
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Seems to be the case I guess….. Trich has a bad rep which is well deserved. What to do with the tub?Would you try and wait a bit and pick the pins or is that foolish? I certainly will monitor any new growth and cut out before sporulation hoping to get something out of this. One poster says that’s fine to wait, but many posters about trich are get it the F out of the house.
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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I waited…trich is overrunning the tub. Lots of yellow fluid which I assume defensive metabolites from the mycelium against the trich. It was pinning nicely. I pulled the pins and some of them have some green on them. Are these safe to use if scraped off? Can I recover anything thing here for use? I am burying the tub tomorrow. 5 months down the trich drain. Probalay contaminated my whole grow area. So frustrating.
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,801
Loc: Germany
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Quote:
Hopefulshroom said: I waited…trich is overrunning the tub. Lots of yellow fluid which I assume defensive metabolites from the mycelium against the trich. It was pinning nicely. I pulled the pins and some of them have some green on them. Are these safe to use if scraped off? Can I recover anything thing here for use? I am burying the tub tomorrow. 5 months down the trich drain. Probalay contaminated my whole grow area. So frustrating.
I´d toss the shrooms that have visibly mold marks. The yellow fluid is antibiotic formed by mycelium to fight off bacteria. Bacteria weakened the myc network, so other nasties can take over. From mold there´s no recovery. Don´t be afraid to ruin your grow room. Unless you have some nasties like lecanicillium (formerly verticillium), just clean the tubs and keep up working. Be anal with the crucial steps to make healthy spawn and you should be good. Mold spores are all around us. By the time you mix your spawn to a tub, there´s nothing to fear. Your strong mycelium fights it´s own battle now. In general, if you see trich early on, you need to work on these crucial steps 
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johnukguy
Learning



Registered: 06/23/22
Posts: 2,423
Loc: Colorado
Last seen: 1 year, 2 months
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Next time post pics as you were asked to. It helps others to help you.
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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I did not think pics would help at this point the green monster was everywhere- not a green carpet- but certainly enough to realize the tub is gone. I buried it today. Maybe the cooler nights and other biotic in soil, can give them mycelium a fighting chance. There was a lot of yellow anti trich fluid. I thought there looked like there was a difference in mycelial architecture during colonization but I have no experience so I assumed that what I saw was just natural variance. The tufts I saw did end up as pins.Five months down the drain- how do you folks bear it? I had no idea that this would take this long. Unfortunately I do not know anyone so I have no choice by to try again. Brown rice mix in jars with spore syringe have better chance of success? I am done with spawn bags!
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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So the one monotub failed- five months gone, two stalled spawn bags and a trich infested monotub. Pins look to cobweb looking mycelium on the exterior. Scraping this off is probably futile as I imagine the hyphae have likely penetrated the pin itself. Eating these is probably stupid? Very discouraging to say the least. What tek do you think will be close to fool proof? Spawn bags and spore syringe is not something I want to try again.
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,340
Last seen: 10 months, 7 days
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PFTEK or agar to grains
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PBJ710
Strangler


Registered: 07/05/19
Posts: 3,301
Last seen: 6 hours, 35 minutes
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Use agar to clean up your culture before you expand it or use PFTek jars/plates for MS. Anything else will have a high chance of failure.
Show pics of the fruits if you want feedback on them. New cultivators have a tendency to inaccuratly describe things...
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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Re: Mono tub newbie [Re: PBJ710]
#28317210 - 05/12/23 01:13 PM (1 year, 8 months ago) |
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I have a dissection scope and I could not get a pic. I might try a couple of things later. The largest pin has a net of mycelium over it. It is 2” dry. White stuff seems independent of the flesh of the pin. Seems like overgrowth, unless that is normal before the cap starts to spread. What a waste….?
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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Ok- still can’t get a pic, but I studied my spawn bag stall pins versus the triched monotub pins. There is a white covering on both caps. The monotub has these blue rope like structures that appear like roots that go off axis from the stipe of the pin. They are all cracker dry now. This randomized arrangement might be trich I am thinking. It would be tedious, but is it worth scraping that off and would it be safe? I am assuming the downy white over the pin/cap is normal stuff or yet a different mold. The dark stuff is thicker and not tightly woven. Like tree roots cracking into a sidewalk type arrangement. I suppose I should cut the stipe and seem if that is internal or is this all a waste of time? I have wasted five months so far, so a an hour of two of surgery might be worth it.
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fiddle_head
fettucine embargo



Registered: 08/05/08
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Loc: moon
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None of that time is wasted if you learned and gained experience. I spent years figuring shit out before I became confident.
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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Understood- I think I should have opened up the tub and fixed the liner much earlier. Moisture was too high. May not have helped though as the trich was pretty much everywhere. Still not sure what do with pins.
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NoChill
Señor Siesta


Registered: 01/03/18
Posts: 159
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I want to be sure that you hear all the people telling you that your spawn was bad from the very beginning. You didn't get trich from not cutting or taping your liner or whatever. It was from your dirty spawn. Period.
Agar is fun and actually not hard. PF tek is finicky and laborious. Those are my opinions, and your two best noob-friendly, acceptable options.
Keep up the good work!
-NC
-------------------- Agar Saves!
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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Re: Mono tub newbie [Re: NoChill]
#28344268 - 06/02/23 10:02 AM (1 year, 7 months ago) |
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My buried triched out monotub seems to be showing some pins- they look a bit weird and where the soil washed off I see some nasty green. I don’t think it likely that trich could be interwoven inside these pins, but the morphology is not typical. Are these safe?
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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So I am wondering if because of the watering that another shroom is growing in between the pieces of the monotub cake as well. Going to wait a day and see if they grow more.
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Hopefulshroom
Stranger

Registered: 04/12/23
Posts: 54
Last seen: 7 months, 6 days
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I don’t like the look of these. Close inspection looks like there could be trich overgrowth. Hard to tell. Thoughts?
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