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milkboy
Child



Registered: 05/01/21
Posts: 2,314
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Re: SEMI-SOLID LIQUID CULTURE [Re: Pandaskis]
#28269632 - 04/09/23 07:27 AM (10 months, 3 days ago) |
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Quote:
Pandaskis said: I wonder if you can sub the agar for gelatin, that way you can make it more solid initially and as the mycelium eats the gelatin it will become more watery. Wonder if its possible to spot contam in its more solid form before the mycelium turns it watery.
Just some crazy thoughts haha
That would defeat the purpose of using sslc
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Pandaskis
Eating Bamboo



Registered: 03/14/23
Posts: 1,869
Last seen: 1 month, 21 days
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Re: SEMI-SOLID LIQUID CULTURE [Re: milkboy]
#28269634 - 04/09/23 07:28 AM (10 months, 3 days ago) |
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I mean perhaps you can use it before its fully liquified haha, and still semi solid as i said, crazy untested thoughts :P
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Smoothcat
Renegade-master



Registered: 10/07/15
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#28322845 - 05/17/23 06:53 AM (8 months, 27 days ago) |
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Gonna use my first LC today in a bag (always be baggin ) the bag has been sat for the best part of a year in a tote. I just put it in the PC again last night as who knows how sterile it could be at this point. I think the grains have dried a little over time as well so hopefully the LC will add a little extra hydration
Anyway was looking at stuff for using LC and came across this thread so just wanted to get onto it and will probably be giving it a go after I pour out the other LC
Nice one Nate - I do like your teks 
Need to get my supporter status back though as cant post pics at the moment
-------------------- Back once again with the ill behaviour Links I like
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: SEMI-SOLID LIQUID CULTURE [Re: Smoothcat]
#28322931 - 05/17/23 08:23 AM (8 months, 27 days ago) |
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Nice dude hope it goes well
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altford78
What do I put here

Registered: 05/09/23
Posts: 890
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Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
#28339475 - 05/29/23 01:33 PM (8 months, 15 days ago) |
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How long do wait after inoculation to put it on the stir plate? 24-36hours?
I did two 200ml media bottles on Sunday, one with 0.1% agar and the other with 0.2%. The 0.1% is pretty much water like, the 0.2% is kinda syrupy but the stir plate manages to make an almost full vortex at 2000rpm.
0.2% LME ends up crystal clear, I might need to up that a little bit, I suspect my LME is on the weak end.
Awesome thread.
-------------------- Mold cultivator extraordinaire I also dabble in bacteria I'm also a retard sometimes
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altford78
What do I put here

Registered: 05/09/23
Posts: 890
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Re: SEMI-SOLID LIQUID CULTURE [Re: altford78]
#28340518 - 05/30/23 11:11 AM (8 months, 14 days ago) |
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It's been almost 48h since inoculation, I put the stir plate for 10mins, came back and holy shit that's a lot of bubbles.
They are going to go away right? Right?
-------------------- Mold cultivator extraordinaire I also dabble in bacteria I'm also a retard sometimes
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Bobgas
Master of Mistakes

Registered: 09/01/22
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Re: SEMI-SOLID LIQUID CULTURE [Re: altford78]
#28340595 - 05/30/23 12:42 PM (8 months, 14 days ago) |
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Dunno - I dont use stir plates. I swirl a bit each day (sometimes miss a day.) I just didn't worry about it.
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,317
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Re: SEMI-SOLID LIQUID CULTURE [Re: Bobgas]
#28340610 - 05/30/23 12:54 PM (8 months, 14 days ago) |
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Quote:
Bobgas said: Dunno - I dont use stir plates. I swirl a bit each day (sometimes miss a day.) I just didn't worry about it.
Same didnβt matter. I did mine in a media bottle and Iβd shake that bitch like it was a grain jar
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aPurpleCray0n
His Dudeness



Registered: 02/13/23
Posts: 290
Loc: π¨π¦
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Re: SEMI-SOLID LIQUID CULTURE [Re: Screwup]
#28340668 - 05/30/23 01:40 PM (8 months, 14 days ago) |
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You just leave your lids cracked for ge?
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,317
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Quote:
aPurpleCray0n said: You just leave your lids cracked for ge?
Truthfully Iβve only done SSLC one time. I kept the lid cracked and had cling wrap around it just incase lmao. But yes.
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altford78
What do I put here

Registered: 05/09/23
Posts: 890
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Re: SEMI-SOLID LIQUID CULTURE [Re: Screwup] 1
#28340703 - 05/30/23 02:07 PM (8 months, 14 days ago) |
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I have mine tight like a fish's asshole. Can't be fucked to deal with loose lids right now, I'll probably crack it an a couple days. Or not, we'll see. I remember reading somewhere that one guy had it tight and it made no difference.
Man those bubbles aint going nowhere. I fucked up, shouldn't have left it on the stir plate and left. How the fuck am I going to see anything in this lol.
-------------------- Mold cultivator extraordinaire I also dabble in bacteria I'm also a retard sometimes
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aPurpleCray0n
His Dudeness



Registered: 02/13/23
Posts: 290
Loc: π¨π¦
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Re: SEMI-SOLID LIQUID CULTURE [Re: Screwup]
#28340980 - 05/30/23 06:47 PM (8 months, 14 days ago) |
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Quote:
Screwup said:
Quote:
aPurpleCray0n said: You just leave your lids cracked for ge?
Truthfully Iβve only done SSLC one time. I kept the lid cracked and had cling wrap around it just incase lmao. But yes.
Awesome, Iβve got a clone culture Iβve been working on I want to try this with
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Yuggoth
Mi-Go Cultivator



Registered: 03/04/23
Posts: 496
Loc: Lost Carcosa
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If you heat the jar in a water bath, the mixture will loosen up and the bubbles can rise to the top more easily. How hot can the mycelium take without harm? 90F maybe? Precise warming would be easy to do with a sous vide cooker.
Or maybe we are witnessing the birth of... BLUBBLE TEK.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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Phat69
Stranger

Registered: 11/11/11
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Re: SEMI-SOLID LIQUID CULTURE [Re: Yuggoth]
#28341635 - 05/31/23 09:05 AM (8 months, 13 days ago) |
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I have my grain water sslc proofing out right now I like this tek.
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altford78
What do I put here

Registered: 05/09/23
Posts: 890
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Re: SEMI-SOLID LIQUID CULTURE [Re: Yuggoth]
#28343962 - 06/02/23 02:24 AM (8 months, 11 days ago) |
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I actually put it back on the stir plate at a lower rpm for 40mins and all the bubbles migrated to the surface. I then shook it gently by hand and the bubbles went back under the surface. So there's an easy fix if you want to clear them. I can see the myc growing so I don't really mind them anymore.
Side question, I've seen people that have their LC on the stir plates like 24/7. Is there a point on doing this? Doesn't the myc need some time to rest?
-------------------- Mold cultivator extraordinaire I also dabble in bacteria I'm also a retard sometimes
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Shrimps
Traveler


Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: SEMI-SOLID LIQUID CULTURE [Re: altford78]
#28343968 - 06/02/23 02:29 AM (8 months, 11 days ago) |
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it's just energy waste, I wouldn't do that at all. I work with lc/sslc without stirplate
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,317
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Re: SEMI-SOLID LIQUID CULTURE [Re: Shrimps]
#28343969 - 06/02/23 02:31 AM (8 months, 11 days ago) |
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Quote:
Shrimps said: it's just energy waste, I wouldn't do that at all. I work with lc/sslc without stirplate
Yeah just likeβ¦swirl it lol.
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altford78
What do I put here

Registered: 05/09/23
Posts: 890
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Re: SEMI-SOLID LIQUID CULTURE [Re: Screwup]
#28343976 - 06/02/23 02:45 AM (8 months, 11 days ago) |
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Yeah that's what I was thinking as well, what's the point of having them spin all the time.
I don't remember where I saw it, maybe its was one of the youtube pros 
Dude had like a dozen stirplates just spinning.
-------------------- Mold cultivator extraordinaire I also dabble in bacteria I'm also a retard sometimes
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Yuggoth
Mi-Go Cultivator



Registered: 03/04/23
Posts: 496
Loc: Lost Carcosa
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Re: SEMI-SOLID LIQUID CULTURE [Re: altford78]
#28344273 - 06/02/23 10:08 AM (8 months, 11 days ago) |
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In yeast anyway, stirring definitely makes the yeast grow faster. But yeast is not mycelium. Maybe it doesn't help at all. But even if the stir plate has no effect on growth rate, it does keep the mycelium from clumping up, which is nice.
And, when you are making jars of LC, it's nice to spin the ingredients with a stir plate instead of a spoon.
I will keep using my stir plate since I have it and I like it, but I would agree it is not critical equipment.
-------------------- We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo
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altford78
What do I put here

Registered: 05/09/23
Posts: 890
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Re: SEMI-SOLID LIQUID CULTURE [Re: Yuggoth]
#28344371 - 06/02/23 11:49 AM (8 months, 11 days ago) |
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I have a plate and I like using too, I just was wondering why I read/saw something about having them spin all the time (or something like 10h a day). Isn't like even 5mins a day enough? or even 1min
-------------------- Mold cultivator extraordinaire I also dabble in bacteria I'm also a retard sometimes
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