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Invisiblemushhead
Livonia Wanderer
I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: Pandaskis]
    #28335564 - 05/26/23 12:52 PM (1 year, 7 months ago)


The little plant is looking alright.
The mycelia in the last two pics is reacting to a bacteria that is present, this is perhaps why the plant is alive still.
The battle between the bacteria and mycelia is providing the plant with food.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Invisiblemushhead
Livonia Wanderer
I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: mushhead]
    #28336777 - 05/27/23 10:31 AM (1 year, 7 months ago)


Update on the larger tub, it's doing pretty well.
I would get a better pic but it's on the bottom of a few tubs and I'm lazy.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Invisiblemushhead
Livonia Wanderer
I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: mushhead]
    #28340850 - 05/30/23 04:14 PM (1 year, 7 months ago)


Here's some updated pics of the tubs.
During this process I have reached my goal.
One of the tubs has already given fruits and I have collected a clean spore print.
I followed basic procedure to take a print, one print was done for 6hrs, the second for 24hrs. (https://www.shroomery.org/forums/showflat.php/Number/24461957)
6 Hour Print:

The 6hr Print I really didn't care for so it got folded a bit funky and tossed into my emergency spore collection.

24 Hour Print:

This is the print I used to make my plates.

I then made up Four spore plates.


Now we've reached a critical stage of this log.
I have gotten my print, my plates look clean, and I now have a pretty clean culture.
From here I intend to take transfers, propagate onto grain, and my next flushes with this culture will end this log.
Stay tuned!

:teabird:

Edited by mushhead (05/30/23 04:44 PM)

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Invisiblemushhead
Livonia Wanderer
I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: mushhead]
    #28346770 - 06/04/23 08:28 AM (1 year, 7 months ago)

Alright! Another full tub update! Here are all the tubs from the jars that passed the contam check.(I say passed but what I really mean is "good enough")
You will notice that some of them are having bacterial issues, though that does not matter to me.
I am just cultivating this out to clean up the cultures by collecting spore for agar work as previously stated.


You can see from the above that spore syringe to grain is a bad idea.
However, since grain was at the time my only option to cultivate with, and because I did not have the funds to acquire additional equipment I worked with what I had.
I expect within the next couple of days that these tubs mature out and I'll toss up pics of them fully fruited off.
There are the exceptions:

This is the only good tub out of the whole lot that looks at all healthy enough to keep around for a second flush.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

Edited by mushhead (06/04/23 08:45 AM)

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InvisibleProlific Koi
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Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 1
    #28347055 - 06/04/23 12:47 PM (1 year, 7 months ago)

Beautiful coloration! Great work on this thread Mush, excited to follow.

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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: Prolific Koi] * 1
    #28347921 - 06/05/23 07:55 AM (1 year, 7 months ago)

Just a quick update on the one good tub out of the whole lot:

After a little under ~20hrs and she's looking gorgeous as hell.

Additionally the other tubs are doing alright as well, except they're just slower due to the bacteria present, though I will say still quite as beautiful.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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InvisibleAyePlus
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Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 2
    #28347940 - 06/05/23 08:13 AM (1 year, 7 months ago)

Either the clones/ cultures are bunk or something is off with your suface conditions, i think its the latter,

Even with slightly bacterial sub You should be seeing much more even fuller pinsets,
smaller tubs like shoeboxes were always harder for me to maintain, you might need thicker subs or just a little less fae. I

see a few that look good but most are on the dry side, one or two looks to have been sprayed/slightly matted but still on the dryish side,

I also find i get better pinsets when my top layer is thicker, but not super compressed. And semi controversial opinion, a straight verm casing is easy af and can dramatically inprove surface conditions in less than ideal climates, same with true casings, neither are necessary but can both make a huge difference in the right situation


Good start! Best of luck moving forward


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: AyePlus] * 1
    #28347952 - 06/05/23 08:27 AM (1 year, 7 months ago)

Quote:

AyePlus said:
Either the clones/ cultures are bunk or something is off with your suface conditions, i think its the latter,

Even with slightly bacterial sub You should be seeing much more even fuller pinsets,
smaller tubs like shoeboxes were always harder for me to maintain, you might need thicker subs or just a little less fae. I

see a few that look good but most are on the dry side, one or two looks to have been sprayed/slightly matted but still on the dryish side,

I also find i get better pinsets when my top layer is thicker, but not super compressed. And semi controversial opinion, a straight verm casing is easy af and can dramatically inprove surface conditions in less than ideal climates, same with true casings, neither are necessary but can both make a huge difference in the right situation


Good start! Best of luck moving forward



Thanks for the advice! These tubs are sitting in a grow room that has constant air flow due to running a carbon filter within the room itself.
The jars for these were bacterial(this was spore syringe to grain) and when pressed upon by my finger are fairly wet, although a couple were on the dry side this morning when I took the pics.
The bacteria is slowing things down, no matter how hydrated the tubs are.
I used a pseudo casing layer for each of these tubs and I'm really not wanting to use vermiculite anymore simply due to the amount of dust and debris it causes.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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InvisibleAyePlus
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Registered: 12/18/14
Posts: 3,393
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Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 1
    #28348042 - 06/05/23 09:53 AM (1 year, 7 months ago)

For sure, Maybe just try goin thicker with the too layer/pseudo. And bacterial spawn does go slower especially in wetter subs, I don’t compress it at all, spawn it on the dryer side and spray just the top layer after spawning to compensate and to avoid overdrying … then check it and mist as knotting or bottom water when the pins are in.

Keep dialing in conditions and cultures, seems like you already know spores to grain is less than ideal so but faht makes it work, i’m an agar guy but if i werent, i’d rather make a small brf cake and transfer a pin clone to another and one more transfer before slurrying that to grain.

Another thought. The recirculating fan on carbon filter may be giving too much airflow but not providing enough fresh air, unless you also have an intake/exhaust setup too..


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: AyePlus]
    #28348070 - 06/05/23 10:07 AM (1 year, 7 months ago)

The room is set up with external ventilation so fresh air is constantly being cycled through the room itself.
The filter is in place to capture scents from herbs growing nearby.
I don't compress my substrates either, usually I try to leave them as fluffy as I can.
I've been around for some time, I know faht makes spore syringe to grain work and I've done it multiple times before.
It can be a serious shit show and I'm fairly lucky that it didn't turn into a big green mess this go around. I'm not afraid of a wee bit of bacteria.
You perhaps maybe want to go back to the first page and read the whole log :laugh:


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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InvisibleAyePlus
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Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 1
    #28348246 - 06/05/23 01:06 PM (1 year, 7 months ago)

Theres only one page and i read the whole thing, i just teread it to see if there was anything I missed, you didnt specify whether you compressed and your photo shows your top layer super thin, your pinsets are weak even for bacterial sub and im seeing alot of aborts that all point to me with issues with airflow. so i was merely offering suggestions about how I handle it…

But  if you arent looking for input and more i interested in keeping a log of your grow then perhapsin the future a journal entry would be a better place for this as opposed to the open forum... I’ll see myself out

:highfive:

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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: AyePlus] * 1
    #28348259 - 06/05/23 01:20 PM (1 year, 7 months ago)

Quote:

AyePlus said:
Theres only one page and i read the whole thing, i just teread it to see if there was anything I missed, you didnt specify whether you compressed and your photo shows your top layer super thin, your pinsets are weak even for bacterial sub and im seeing alot of aborts that all point to me with issues with airflow. so i was merely offering suggestions about how I handle it…

But  if you arent looking for input and more i interested in keeping a log of your grow then perhapsin the future a journal entry would be a better place for this as opposed to the open forum... I’ll see myself out

:highfive:



I appreciate your feed back and I was in no way being rude my friend. I took your advice to heart and I even admitted that some were dry as you had stated.
I in no way wanted to make you feel as though you were wasting your time and I enjoy the feedback.
If I wanted to a journal-log I would have done it that way to begin with <3
:shrug:
EDIT:
I also agreed with you that you should make your subs a bit fluffy, which is what I do, and indeed a thicker case would be preferable.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

Edited by mushhead (06/05/23 01:28 PM)

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InvisibleProlific Koi
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Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 2
    #28348298 - 06/05/23 01:48 PM (1 year, 7 months ago)

Haha do my forum next AyePlus?

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InvisibleAyePlus
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Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 1
    #28348306 - 06/05/23 02:01 PM (1 year, 7 months ago)

Quote:

mushhead said:





I apologize if I took it the wrong way when you suggested i scroll to the top and read it again


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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OfflinePandaskis
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Re: Musheads 2023 B+ Grow Log [Re: AyePlus] * 2
    #28348314 - 06/05/23 02:07 PM (1 year, 7 months ago)

Nothing to see here, just civil adults being civil, love it

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Invisiblemushhead
Livonia Wanderer
I'm a teapot


Registered: 08/22/14
Posts: 2,242
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Re: Musheads 2023 B+ Grow Log [Re: AyePlus] * 1
    #28348551 - 06/05/23 05:18 PM (1 year, 7 months ago)

Quote:

AyePlus said:
Quote:

mushhead said:





I apologize if I took it the wrong way when you suggested i scroll to the top and read it again



No worries! I honestly thought you hadn't noticed the front page and thought perhaps you missed my agar plates and such lol.
Truly my dude I appreciate your advice, in fact I actually cleared those subs and began a soak to re-hydrate for perhaps a larger second flush.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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Invisiblemushhead
Livonia Wanderer
I'm a teapot


Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452
Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 1
    #28352487 - 06/08/23 06:45 PM (1 year, 7 months ago)


Overall the harvest has been nice, dried weight at about 408g rounded down.
Most of the fruits I've harvested have been fairly chunky with solid stipes and thick caps.
During this time I have taken clones, spore prints, and will continue propagation.

I did re-hydrate all my tubs, I allowed them to sit in distilled water until soaked(10hrs), then drained.
I have already observed pins and an additional flush coming off most of them, will post pics soon.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

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InvisibleProlific Koi
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Re: Musheads 2023 B+ Grow Log [Re: mushhead] * 1
    #28363342 - 06/17/23 01:59 PM (1 year, 6 months ago)

:threadmonitor:

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Invisiblemushhead
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Registered: 08/22/14
Posts: 2,242
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Re: Musheads 2023 B+ Grow Log [Re: Prolific Koi] * 1
    #28363590 - 06/17/23 06:16 PM (1 year, 6 months ago)

Alright! So it's been a second since I posted an update.
Here are the spore swab plates from this grow now. You'll notice wedges have been taken.


Here are the first transfer plates. You can see wedges have also been taken from these.
Don't worry I saw the bacteria, it is the main reason why I transferred from that plate so early.


And here we have second transfers.


Soon these will be going to grain and the final culmination of this log will be those final tubs.

Here are the surviving test plates. The others will not be shown as they have been disposed of.
2% LME to 500ml water is your best bet with agar.



--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room

IRC: ##mycology on Libera.chat come hang!

Edited by mushhead (06/17/23 07:04 PM)

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InvisibleProlific Koi
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Registered: 12/26/22
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Re: Musheads 2023 B+ Grow Log [Re: mushhead]
    #28364709 - 06/18/23 08:05 PM (1 year, 6 months ago)

Looking good mush! 2% without agar just lme?

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