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matthew2703
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Registered: 04/21/23
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Loc: Poland
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Can i make gallons of lc from one lc syringe?
#28321789 - 05/16/23 10:22 AM (1 year, 7 months ago) |
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Good morning, good afternoon and good evening whereever you are. So, as i title, can i make few jars of lc from one lc syringe, by adding few cc's of it to pressure cooked water with honey? Will it grow further and further in jars, or won't it work like I think ?
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KnateKing89
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Registered: 06/29/22
Posts: 110
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Re: Can i make gallons of lc from one lc syringe? [Re: matthew2703]
#28321804 - 05/16/23 10:34 AM (1 year, 7 months ago) |
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You can expand LC.
However I would reconsider expanding it into one, one gallon container for a couple of reasons.
1 If the LC goes bad you have to throw out the entire gallon
2 Depending on how you are inoculating your spawn working with an entire gallon would be a pain in the ass.
If using syringes to extract your LC over and over and over there is a good chance one of those times you will introduce bacteria into the LC.
My recommendation and you can take it or leave it is this.
Use the LC to make up a master Agar plate for making more LC in the future. After the agar plate grows out a bit and you are sure it is clean put the rest of the syringe to spawn jars. Grain to grain transfer those jars to more jars before spawning to bulk.
Consider making a master slant if you really like the culture and you can continue to use it for years to come. Done this way you should never have to buy another LC of that culture ever again.
Edit: sorry re read and you were not talking about making a full gallon in one jar. Yes what you are talking about will work.
Edited by KnateKing89 (05/16/23 10:35 AM)
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matthew2703
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Re: Can i make gallons of lc from one lc syringe? [Re: KnateKing89]
#28321825 - 05/16/23 10:50 AM (1 year, 7 months ago) |
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Yea, i also was thinking about making agar plates, but i read that without very sterile environment it have no sense to even try it and i am not ready yet to build a whole flow hood, that's why i thought about just expanding liquid culture that I bought online, which i am sure that is not contaminated to a few jars and store it for later. It is much cheaper and easier for newbie like me. Thanks a lot for answer tho.
Edited by matthew2703 (05/16/23 10:51 AM)
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KnateKing89
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Registered: 06/29/22
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Re: Can i make gallons of lc from one lc syringe? [Re: matthew2703]
#28321834 - 05/16/23 10:55 AM (1 year, 7 months ago) |
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You don't need a flow hood. SAB is all you need. You could buy a plastic tote for $10 or even use a cardboard box. I would not recommend expanding the LC outside of an SAB.
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matthew2703
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Registered: 04/21/23
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Loc: Poland
Last seen: 1 month, 12 days
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Re: Can i make gallons of lc from one lc syringe? [Re: KnateKing89]
#28321842 - 05/16/23 11:04 AM (1 year, 7 months ago) |
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Yeah, I'm hoping to start building a sab this week, i already bought 80l box, just need some time after work, and i will definitely expand this lc in sab if u say so, i also got a silicone for injection ports, so i will try to keep everything as sterile as i can.
Oh and by the way, do you maybe know how this tek is called, i was trying to find it on forum but ended unsuccessfully, or do you have link by any chance to guide :/
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KnateKing89
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Registered: 06/29/22
Posts: 110
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Re: Can i make gallons of lc from one lc syringe? [Re: matthew2703]
#28321882 - 05/16/23 11:40 AM (1 year, 7 months ago) |
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Throwaway
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Registered: 04/22/21
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Re: Can i make gallons of lc from one lc syringe? [Re: matthew2703] 1
#28321979 - 05/16/23 01:09 PM (1 year, 7 months ago) |
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LC is crazy sensitive to contamination so be wary! Good luck and welcome to the Shroomery. Also don't expand into a gallon. Having a gallon anything inside your SAB is just gonna be a pain in the ass and make you fuck up. Imagine having to tilt over the gallon jar with one hand to suck up 10ml of LC with the other. It's just not gonna work.
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matthew2703
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Registered: 04/21/23
Posts: 22
Loc: Poland
Last seen: 1 month, 12 days
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Re: Can i make gallons of lc from one lc syringe? [Re: matthew2703]
#28372663 - 06/24/23 12:16 PM (1 year, 6 months ago) |
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Update In the end I made about 800 ml in three jars. I don't know what this tek is called, but I simply added 3ml of healthy liquid culture purchased online to a mixture of water and honey (96-4). Of course, everything was done under sterile conditions, in a still air box, and the injections were done through an injection port (made from red rtv silicone). Each of the jars came out healthy, below are photos of the final result.

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