|
Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
|
34 Aloha shiitake cultures 2
#28303995 - 05/02/23 07:39 PM (8 months, 22 days ago) |
|
|
So back in 2011 a Groupe buy of Shiitake cultures from Aloha Medicinals happened & Lipa killed it making everyone involved slants.
Dragonaut was one of the members involved & stored his amazing in their original packaging in the refer.
He intrusted them to me to breath back life. Finally I am getting 34 to leap off.
Original thread https://www.shroomery.org/forums/showflat.php/Number/14756674#14756674
Will they still fruit ?
Looking for a couple experienced members to sign on to this task of finding out.
Maby 5 to 10 members willing to try out 2 to 5 of each. Sure different fruiting conditions & Temps freezing substrate ect will effect results & vigor so overlap is good.
You will receive all 34 If you wish, just please run a couple of the lesser known along with the 3782, 75 ect.
In the coming weeks I will prepare .5 ml sets of ependorfs but would like this amazing community to help try em & see if they are still viable.
A complete, updated list will be posted this weekend.
Post here if intrested & when ready I will prepare vials. Prob 2 to 3 weeks as they just literally leaped off this am & a clean transfer to a new nutritional plate is needed.
I will scratch out those that did not make the Journey by this weekend.
1 - GL - P3 2 - SAM'S - P19 3- AUG - P4 4 - CS2 - P16 5 - DON - P4 6 - JP2 - P4 7 - UK7 - P4 8 - UK5 - P4 9 - UK9 - P4 10 - HO1 - P4 11 - CA - P5 12 - CS - P19 13 - 2001 - P17 14 - AH FOOK - P14 15 - F - P3 16 - STRAW - P22 17 - U8 - P10 18 - JUMBO - P20 19 - 3782 - P4 20 - 4015 - P15 21 - TL2 - P4 22 - GE2 - P22
23 - ED - P3 Bacterial beyond rescue 24 - PO1 - P4 25 - CS3 - P10 26 - AM1 - P11 27 - UK8 - P4
28 - UK6 - P4 slow going to try again 29 - 7402 - P4 30 - SX14 - P3 31 - A10 - P23 32 - 75 - P14 33 - EN2 - P4 34 - QUA GU - P15 35 - US1 - P4 36 - HUN - P4 37 - LE1 - P4
38 - PL - P5 Bacterial beyond rescue 39 - IS4 - P4
40 - 720 - NOT READY Missing
Edited by Buddaking (05/08/23 08:37 PM)
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28304090 - 05/02/23 08:38 PM (8 months, 22 days ago) |
|
|
This is amazing.
--------------------
|
Seeker2be
Stranger



Registered: 06/10/13
Posts: 233
Last seen: 39 minutes, 5 seconds
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28304268 - 05/02/23 11:11 PM (8 months, 22 days ago) |
|
|
Id like to join this. PM Me
|
HomerSimpson
Stranger

Registered: 09/03/22
Posts: 40
Last seen: 6 days, 22 hours
|
Re: 34 Aloha shiitake cultures [Re: Seeker2be]
#28304388 - 05/03/23 04:25 AM (8 months, 22 days ago) |
|
|
I would try 2 or 3 random cultures. Let me know. Thx.
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 12 minutes, 8 seconds
|
|
Blast from the past. I would think they would have lost some vigor not being in cryo but it will be cool to see. Good luck
|
Harry Manbach
Lock the door,Kill the light


Registered: 09/04/20
Posts: 339
Loc: 6
Last seen: 2 months, 21 days
|
|
I could toss my hat in this and run a few strains. 4-5 would be reasonable in the grow space I have.
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
|
I would love to be a part of this.
|
spitzenkorper
dabberwook


Registered: 06/01/17
Posts: 189
Loc: bout tree fiddy
Last seen: 2 months, 23 hours
|
|
Whoa! All diff shiitakes?? I’ll try a few
-------------------- Trade List seeds, spores, & more!
|
dyel


Registered: 10/15/21
Posts: 920
Last seen: 12 hours, 57 minutes
|
|
nvm me just here to watch
|
Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
|
Re: 34 Aloha shiitake cultures [Re: dyel] 1
#28311909 - 05/08/23 08:06 PM (8 months, 16 days ago) |
|
|

Got busy. Going to Transfer tomorrow AM.
Updating 1st post on survivors shortly.
Adding my own 3790 along with unknown I call matsc as that was user here I got it from.
--------------------
 AMU Q & A
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28313300 - 05/09/23 07:43 PM (8 months, 15 days ago) |
|
|
Amazing work.
|
Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
|
|
OK so I got a little behind on this project but wrapped up the culture aspect this Morning!
4015 & UK6 did not make the cut as if they refuse to show me vigor on multiple agar mixes their is no reason to continue with a lame horse.
I have 5 sets of .5 ependorfs to share & will be back in town early next week.
All cultures are now slanted 4 different ways 1 grain master of each colonizing to knock up 1 HWFP bag & 200 wood dowells. Fresh plate of each in cold storage to come back to if vigor in my environment at my Temps is achieved indoor.
20 scrub Oaks just fallen & 8k wood dowells ordered for outdoor logs
Dug out the laminator to make a weather resistant lable for each log pile.
--------------------
 AMU Q & A
|
Harry Manbach
Lock the door,Kill the light


Registered: 09/04/20
Posts: 339
Loc: 6
Last seen: 2 months, 21 days
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28328231 - 05/21/23 01:45 AM (8 months, 4 days ago) |
|
|
Quote:
Buddaking said: OK so I got a little behind on this project but wrapped up the culture aspect this Morning!
4015 & UK6 did not make the cut as if they refuse to show me vigor on multiple agar mixes their is no reason to continue with a lame horse.
I have 5 sets of .5 ependorfs to share & will be back in town early next week.
All cultures are now slanted 4 different ways 1 grain master of each colonizing to knock up 1 HWFP bag & 200 wood dowells. Fresh plate of each in cold storage to come back to if vigor in my environment at my Temps is achieved indoor.
20 scrub Oaks just fallen & 8k wood dowells ordered for outdoor logs
Dug out the laminator to make a weather resistant lable for each log pile.

absolutely AMAZING
|
Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
|
|
So it looks like they started landing. I will update the 1st post tomorrow with ones that did not make the cut & replacement #'s for cultures added
--------------------
 AMU Q & A
|
Dirtnapz
Cake Kingdom



Registered: 02/22/23
Posts: 338
Loc: Lurkin yo Threads
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28346722 - 06/04/23 07:10 AM (7 months, 21 days ago) |
|
|

Awesome work, this is great!
-------------------- Extinction is forever
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28347598 - 06/04/23 09:29 PM (7 months, 20 days ago) |
|
|
If I recall correctly, the "missing" 720 culture from the original project was a late arrival, and was not shipped with the rest of the collection. To the best of my knowledge, I had discarded it after some time along with other things stored separately that did not appear to age well. Sorry about that.
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut]
#28354814 - 06/10/23 07:07 PM (7 months, 14 days ago) |
|
|
Is there some info on each of these? I’ve got about ten or so onto agar, but was wondering if some are better suited to indoor vs log grows. I know there are warm weather strains, but not which is what. I can find no info on the aloha site. Wondering if there is some info out there already, or if we’ll just discover as we go.
|
Harry Manbach
Lock the door,Kill the light


Registered: 09/04/20
Posts: 339
Loc: 6
Last seen: 2 months, 21 days
|
|
Quote:
RoscoeReturns said: Is there some info on each of these? I’ve got about ten or so onto agar, but was wondering if some are better suited to indoor vs log grows. I know there are warm weather strains, but not which is what. I can find no info on the aloha site. Wondering if there is some info out there already, or if we’ll just discover as we go.
 Just got more agar made and started down this rabbit hole myself.. With little results.
Would love to start a few cultures just based on visual assessment, but do run pretty constant grow conditions and would prefer to apply effort to better suited candidates ... Especially before I give a whack at some log stacks outside!
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
|
Five of these went to grain today. All will be fruited on supplemented sawdust blocks in my tent. I’ll try to get another five to grain next week. Anyone else making any progress?
|
Harry Manbach
Lock the door,Kill the light


Registered: 09/04/20
Posts: 339
Loc: 6
Last seen: 2 months, 21 days
|
|
I'm still on agar with two of them. My plan is to start 2 per week on agar and either slant or liquid culture each variety as they go to grain for longer storage. This way I don't fill all my incubation space and I can have a nice steady fruiting trial down the road, rather than bombarding my grow room all at once.
|
Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
Last seen: 5 months, 29 days
|
|
I remember that thread way back in the day. It was epic! I still have my Phellinus Linteus slant from Aloha.
Edited by Cloneufc (06/24/23 06:14 AM)
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Cloneufc]
#28376880 - 06/27/23 06:16 PM (6 months, 28 days ago) |
|
|
Got some on grain. Five more tomorrow.
75

UK7

AH FOOK

Jumbo

DON
|
deadmandave
Slime


Registered: 02/16/10
Posts: 3,355
Loc:
Last seen: 4 hours, 12 minutes
|
|
Green agar is an interesting choice 
At first glance I thought it was mold.
|
Harry Manbach
Lock the door,Kill the light


Registered: 09/04/20
Posts: 339
Loc: 6
Last seen: 2 months, 21 days
|
|
Quote:
RoscoeReturns said: Got some on grain. Five more tomorrow.
75

UK7

AH FOOK

Jumbo

DON

Looking great! I've been grabbing cultures out by random, but so far I've got DON, P04,& AM1 on agar.
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
|
Anyone got updates?
--------------------
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 1
#28414716 - 07/31/23 04:04 AM (5 months, 26 days ago) |
|
|
Not anything exciting. Eight varieties on supplemented HWFP colonizing away in bags, eight more on grain, and a final ten on agar. None of the bags have reached 100% colonization yet, so we have a good wait ahead of us.
Edited by RoscoeReturns (07/31/23 04:26 AM)
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
|
Just bumping the thread to check for updates.
--------------------
|
Harry Manbach
Lock the door,Kill the light


Registered: 09/04/20
Posts: 339
Loc: 6
Last seen: 2 months, 21 days
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut]
#28455745 - 09/02/23 08:40 AM (4 months, 24 days ago) |
|
|
I've lost all progress and a lot of bags and grain as well due to a couple of faulty a/c units and some drastic heat issues in the lab/ colonizing room. Doing some remodeling and waiting until cooler temps to start back up on agar.
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 1
#28456265 - 09/02/23 05:20 PM (4 months, 24 days ago) |
|
|
Quote:
Dragonaut said:
Just bumping the thread to check for updates.
I have a dozen sawdust bags looking like popcorn and starting to brown. I have more colonizing, and some grain jars going. All different varieties. I don’t have them all on grain yet, but almost!
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
|

Eight more onto sawdust!
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28477219 - 09/20/23 09:53 PM (4 months, 5 days ago) |
|
|
Hell yeah. I can't wait to see some fruits!
--------------------
|
volleyshroom
Stranger
Registered: 05/14/23
Posts: 21
Last seen: 1 day, 38 minutes
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut]
#28477736 - 09/21/23 02:42 PM (4 months, 5 days ago) |
|
|
This is a really cool project! How did that original group buy work? I'm a newer cultivator and it's encouraging to see group projects that allow for access to such interesting cultures.
|
RedCapRandy
Stranger


Registered: 08/23/23
Posts: 3
Loc: United States
Last seen: 1 month, 9 days
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28516741 - 10/24/23 03:34 PM (3 months, 3 days ago) |
|
|
This seems like an amazing thing going on here. I am very new but would love to help out and give some a try if you would have me. I really am enjoying the enthusiasm so many in this field are to spread what they can to keep them going. Thank you for your consideration.
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: RedCapRandy] 1
#28526887 - 11/02/23 05:19 PM (2 months, 25 days ago) |
|
|
Little update!
Now that we got some cold weather I put six blocks outside to cold shock overnight. Brought them in today and the are soaking. Tomorrow they will get a few slaps and put in the tent.
I lost on block to some contamination. There is about an inch of metabolites in the bottom of the bag, and the block crumbles when touched.
The six that are still going have browned up really nicely.
Two of the blocks have a few fruit in the bag, both LE1 and QUA GU.
The block of 75, the only variety I’ve grown in the past, is quite light and sounds almost hollow.
There are eight more bags that started later. The browning has started, but they’re not quite ready yet. Update and some pics tomorrow!
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28526981 - 11/02/23 06:55 PM (2 months, 24 days ago) |
|
|
Nice work! Are you going to try to clone anything, or take prints?
--------------------
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 1
#28526989 - 11/02/23 07:07 PM (2 months, 24 days ago) |
|
|
Quote:
Dragonaut said: Nice work! Are you going to try to clone anything, or take prints?
I never tried printing shiitake. I like to harvest before they start dropping spore. I’ll clone anything that looks interesting, but I still have plates of all these cultures.
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
|
Six blocks in the tent a few days ago when I unbagged them.

LE1 showing a few pins already.

Qua Gu pinning up, too!
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
Re: 34 Aloha shiitake cultures [Re: Buddaking] 1
#28530668 - 11/05/23 02:01 PM (2 months, 22 days ago) |
|
|
--------------------
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 2
#28537554 - 11/10/23 05:46 PM (2 months, 16 days ago) |
|
|
Bad news, two of my blocks have some mold. Could be bad spawn, or a nasty tent. So 75 and DON are moldy just as they start pinning.
Good news, Qua Gu is looking good.

LE1 is looking really nice.

The other two are beginning to pin as well. These are in a tent @ 65 degrees F and 85% RH. I’ll be eating some shiitake tomorrow!
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
Re: 34 Aloha shiitake cultures [Re: Buddaking]
#28537636 - 11/10/23 06:33 PM (2 months, 16 days ago) |
|
|
this is amazing! Nice job.
--------------------
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 2
#28564256 - 12/01/23 07:14 PM (1 month, 26 days ago) |
|
|
Eight blocks in the tent. I disinfected it after the poor performance last time, and I think it’s much better now. These two have taken off in the last few days.
AM-1

SX-14
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
|
Looking good!
--------------------
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 2
#28568026 - 12/04/23 09:37 AM (1 month, 24 days ago) |
|
|
Love the sombrero look of these.

They are the AM-1 shiitakes.

They covered the bottom and grew through the wire mesh shelf.

1 1/2 lbs from a 3 1/2 lbs block.
Edited by RoscoeReturns (12/04/23 09:41 AM)
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
|
That's awesome! 
What is that...233% B.E. ? That good for shiitake?
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 1
#28574800 - 12/08/23 06:45 PM (1 month, 19 days ago) |
|
|
I don’t know from BE. I know they tasted great.
A pound of 154

And another of AUG
|
deadmandave
Slime


Registered: 02/16/10
Posts: 3,355
Loc:
Last seen: 4 hours, 12 minutes
|
|
Nice. I love shiitake. Looks appetizing
|
Dragonaut


Registered: 06/24/04
Posts: 6,190
|
Re: 34 Aloha shiitake cultures [Re: deadmandave]
#28575183 - 12/08/23 11:44 PM (1 month, 19 days ago) |
|
|
Damn...they do look tasty.
--------------------
|
Phaethon
Stranger
Registered: 07/04/22
Posts: 18
|
Re: 34 Aloha shiitake cultures [Re: Dragonaut] 1
#28597622 - 12/25/23 02:30 PM (1 month, 3 days ago) |
|
|
Very late to the party, but I'd love to have a few cultures if you're willing. I've fruited a supposed S75 culture a few times. Interested to see whether the S75 from Aloha is any better, and I'd love to try 3782 and any random ones we haven't tested yet. I'm planning to focus on shiitake next year, so it'd be awesome to have a few options to see which one works best in my low tech setup (fruiting two blocks per "monotub" now, and hopefully a tent soon, likely without constant humidification or airflow, which seems like it will work fine for at least some shiitake strains).
I'm amazed at your yield with AM-1, RoscoeReturns!
-------------------- LAGM2.023
|
MorePies
Stranger
Registered: 02/09/16
Posts: 808
Loc: TX
Last seen: 1 month, 1 day
|
Re: 34 Aloha shiitake cultures [Re: Phaethon] 1
#28599093 - 12/26/23 08:55 PM (1 month, 1 day ago) |
|
|
For whatever reason, I stopped by the shroomery and saw this thread. As a commercial grower, I got my start into learning here and it really makes me smile to see this piece of mushroom genetic history being revived. I sent lipa a msg, I wonder if he will stop by too.
Thank you.
|
wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,197
Loc: Colonial alley
Last seen: 4 hours, 9 minutes
|
|
holy Shitake
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
|
flugelizor
Furious ball of nothing


Registered: 11/16/08
Posts: 2,107
Loc: Western NY
Last seen: 3 hours, 30 minutes
|
|
Hmm, A blast from the past. (was it really 12 years ago? ) I was a part of this group buy. I was one of the few who actually grew out my assigned strains. https://www.shroomery.org/forums/showflat.php/Number/16800562#16800562
I still have the original packaging and many of the original cultures. (unrefrigerated) So many cultures! I only made masters and refrigerated 3: STRAW, 3782, S75
I have more time on my hands, so I will probably buy a flow hood and revive some.
|
flugelizor
Furious ball of nothing


Registered: 11/16/08
Posts: 2,107
Loc: Western NY
Last seen: 3 hours, 30 minutes
|
|
Great work Roscoe, What was your sawdust recipe?
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 37 minutes, 36 seconds
|
Re: 34 Aloha shiitake cultures [Re: flugelizor] 1
#28604934 - 12/31/23 05:28 PM (27 days, 23 hours ago) |
|
|
Quote:
flugelizor said: Great work Roscoe, What was your sawdust recipe?
Thanks.
I’m making 3 - 4 pound blocks with 20% fuel pellets, 20% soy hulls, 60% water all by weight.
|
|