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InvisibleBuddaking
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34 Aloha shiitake cultures * 2
    #28303995 - 05/02/23 07:39 PM (8 months, 22 days ago)

So back in 2011 a Groupe buy of Shiitake cultures from Aloha Medicinals happened & Lipa killed it making everyone involved slants.

Dragonaut was one of the members involved & stored his amazing in their original packaging in the refer.

He intrusted them to me to breath back life.
Finally I am getting 34 to leap off.

Original thread https://www.shroomery.org/forums/showflat.php/Number/14756674#14756674

Will they still fruit ?

Looking for a couple experienced members to sign on to this task of finding out.

Maby 5 to 10 members willing to try out 2 to 5 of each. Sure different fruiting conditions & Temps freezing substrate ect will effect results & vigor so overlap is good.

You will receive all 34 If you wish, just please run a couple of the lesser known along with the 3782, 75 ect.

In the coming weeks I will prepare .5 ml sets of ependorfs but would like this amazing community to help try em & see if they are still viable.

A complete, updated list will be posted this weekend.

Post here if intrested & when ready I will prepare vials. Prob 2 to 3 weeks as they just literally leaped off this am & a clean transfer to a new nutritional plate is needed.

I will scratch out those that did not make the Journey by this weekend.


1 - GL - P3
2 - SAM'S - P19
3- AUG - P4
4 - CS2 - P16
5 - DON - P4
6 - JP2 - P4
7 - UK7 - P4
8 - UK5 - P4
9 - UK9 - P4
10  - HO1 - P4
11 - CA - P5
12 - CS - P19
13 - 2001 - P17
14 - AH FOOK - P14
15 - F - P3
16 - STRAW - P22
17 - U8 - P10
18 - JUMBO - P20
19 - 3782 - P4
20 - 4015 - P15
21 - TL2 - P4
22 - GE2 - P22
23 - ED - P3 Bacterial beyond rescue
24 - PO1 - P4
25 - CS3 - P10
26 - AM1 - P11
27 - UK8 - P4
28 - UK6 - P4 slow going to try again
29 - 7402 - P4
30 - SX14 - P3
31 - A10 - P23
32 - 75 - P14
33 - EN2 - P4
34 - QUA GU - P15
35 - US1 - P4
36 - HUN - P4
37 - LE1 - P4
38 - PL - P5 Bacterial beyond rescue
39 - IS4 - P4
40 - 720 - NOT READY Missing


Edited by Buddaking (05/08/23 08:37 PM)


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28304090 - 05/02/23 08:38 PM (8 months, 22 days ago)

:heart: This is amazing. :awepreciation:


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OfflineSeeker2be
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28304268 - 05/02/23 11:11 PM (8 months, 22 days ago)

Id like to join this. PM Me


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OfflineHomerSimpson
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Re: 34 Aloha shiitake cultures [Re: Seeker2be]
    #28304388 - 05/03/23 04:25 AM (8 months, 22 days ago)

I would try 2 or 3 random cultures. Let me know.  Thx.


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OfflineStromriderM
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Re: 34 Aloha shiitake cultures [Re: HomerSimpson]
    #28304476 - 05/03/23 06:06 AM (8 months, 22 days ago)

Blast from the past. I would think they would have lost some vigor not being in cryo but it will be cool to see. Good luck


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: HomerSimpson]
    #28304477 - 05/03/23 06:07 AM (8 months, 22 days ago)

I could toss my hat in this and run a few strains. 4-5 would be reasonable in the grow space I have.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Harry Manbach]
    #28304485 - 05/03/23 06:11 AM (8 months, 22 days ago)

I would love to be a part of this.


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Offlinespitzenkorper
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28308432 - 05/06/23 12:01 AM (8 months, 19 days ago)

Whoa!
All diff shiitakes??
I’ll try a few


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Offlinedyel
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Re: 34 Aloha shiitake cultures [Re: spitzenkorper]
    #28308532 - 05/06/23 02:59 AM (8 months, 19 days ago)

nvm me just here to watch :popcorn:


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InvisibleBuddaking
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Re: 34 Aloha shiitake cultures [Re: dyel] * 1
    #28311909 - 05/08/23 08:06 PM (8 months, 16 days ago)



Got busy. Going to Transfer tomorrow AM.

Updating 1st post on survivors shortly.

Adding my own 3790 along with unknown I call matsc as that was user here I got it from.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28313300 - 05/09/23 07:43 PM (8 months, 15 days ago)

Amazing work.


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InvisibleBuddaking
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns] * 2
    #28327513 - 05/20/23 12:03 PM (8 months, 5 days ago)

OK so I got a little behind on this project but wrapped up the culture aspect this Morning!

4015 & UK6 did not make the cut as if they refuse to show me vigor on multiple agar mixes their is no reason to continue with a lame horse.

I have 5 sets of .5 ependorfs to share & will be back in town early next week.

All cultures are now slanted 4 different ways
1 grain master of each colonizing to knock up 1 HWFP bag & 200 wood dowells.
Fresh plate of each in cold storage to come back to if vigor in my environment at my Temps is achieved indoor.

20 scrub Oaks just fallen & 8k wood dowells ordered for outdoor logs

Dug out the laminator to make a weather resistant lable for each log pile.



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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28328231 - 05/21/23 01:45 AM (8 months, 4 days ago)

Quote:

Buddaking said:
OK so I got a little behind on this project but wrapped up the culture aspect this Morning!

4015 & UK6 did not make the cut as if they refuse to show me vigor on multiple agar mixes their is no reason to continue with a lame horse.

I have 5 sets of .5 ependorfs to share & will be back in town early next week.

All cultures are now slanted 4 different ways
1 grain master of each colonizing to knock up 1 HWFP bag & 200 wood dowells.
Fresh plate of each in cold storage to come back to if vigor in my environment at my Temps is achieved indoor.

20 scrub Oaks just fallen & 8k wood dowells ordered for outdoor logs

Dug out the laminator to make a weather resistant lable for each log pile.





:wat: absolutely AMAZING


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InvisibleBuddaking
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Re: 34 Aloha shiitake cultures [Re: Harry Manbach] * 3
    #28346434 - 06/03/23 10:39 PM (7 months, 21 days ago)

So it looks like they started landing. I will update the 1st post tomorrow with ones that did not make the cut & replacement #'s for cultures added


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InvisibleDirtnapz
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28346722 - 06/04/23 07:10 AM (7 months, 21 days ago)

:takingnotes:

Awesome work, this is great!


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28347598 - 06/04/23 09:29 PM (7 months, 20 days ago)

If I recall correctly, the "missing" 720 culture from the original project was a late arrival, and was not shipped with the rest of the collection. To the best of my knowledge, I had discarded it after some time along with other things stored separately that did not appear to age well. Sorry about that.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut]
    #28354814 - 06/10/23 07:07 PM (7 months, 14 days ago)

Is there some info on each of these? I’ve got about ten or so onto agar, but was wondering if some are better suited to indoor vs log grows. I know there are warm weather strains, but not which is what. I can find no info on the aloha site. Wondering if there is some info out there already, or if we’ll just discover as we go.


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28355922 - 06/11/23 05:24 PM (7 months, 14 days ago)

Quote:

RoscoeReturns said:
Is there some info on each of these? I’ve got about ten or so onto agar, but was wondering if some are better suited to indoor vs log grows. I know there are warm weather strains, but not which is what. I can find no info on the aloha site. Wondering if there is some info out there already, or if we’ll just discover as we go.



:whathesaid:
Just got more agar made and started down this rabbit hole myself.. With little results.

Would love to start a few cultures just based on visual assessment, but do run pretty constant grow conditions and would prefer to apply effort to better suited candidates ... Especially before I give a whack at some log stacks outside!


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Harry Manbach]
    #28366089 - 06/19/23 06:39 PM (7 months, 5 days ago)

Five of these went to grain today. All will be fruited on supplemented sawdust blocks in my tent. I’ll try to get another five to grain next week. Anyone else making any progress?


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns] * 1
    #28366634 - 06/20/23 05:25 AM (7 months, 5 days ago)

I'm still on agar with two of them. My plan is to start 2 per week on agar and either slant or liquid culture each variety as they go to grain for longer storage. This way I don't fill all my incubation space and I can have a nice steady fruiting trial down the road, rather than bombarding my grow room all at once.


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OfflineCloneufc
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Re: 34 Aloha shiitake cultures [Re: Harry Manbach]
    #28372298 - 06/24/23 06:14 AM (7 months, 1 day ago)

I remember that thread way back in the day. It was epic! I still have my Phellinus Linteus slant from Aloha.


Edited by Cloneufc (06/24/23 06:14 AM)


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Cloneufc]
    #28376880 - 06/27/23 06:16 PM (6 months, 28 days ago)

Got some on grain. Five more tomorrow.

75


UK7


AH FOOK


Jumbo


DON


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Offlinedeadmandave
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns] * 1
    #28377106 - 06/27/23 09:01 PM (6 months, 28 days ago)

Green agar is an interesting choice :lol:

At first glance I thought it was mold.


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28377697 - 06/28/23 11:40 AM (6 months, 28 days ago)

Quote:

RoscoeReturns said:
Got some on grain. Five more tomorrow.

75


UK7


AH FOOK


Jumbo


DON





Looking great!
I've been grabbing cultures out by random, but so far I've got
DON, P04,& AM1 on agar.


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: Harry Manbach]
    #28414571 - 07/30/23 10:20 PM (5 months, 26 days ago)

Anyone got updates?


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 1
    #28414716 - 07/31/23 04:04 AM (5 months, 26 days ago)

Not anything exciting. Eight varieties on supplemented HWFP colonizing away in bags, eight more on grain, and a final ten on agar. None of the bags have reached 100% colonization yet, so we have a good wait ahead of us.



Edited by RoscoeReturns (07/31/23 04:26 AM)


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28455449 - 09/01/23 09:13 PM (4 months, 24 days ago)

:bump: Just bumping the thread to check for updates.


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: Dragonaut]
    #28455745 - 09/02/23 08:40 AM (4 months, 24 days ago)

I've lost all progress and a lot of bags and grain as well due to a couple of faulty a/c units and some drastic heat issues in the lab/ colonizing room. Doing some remodeling and waiting until cooler temps to start back up on agar.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 1
    #28456265 - 09/02/23 05:20 PM (4 months, 24 days ago)

Quote:

Dragonaut said:
:bump: Just bumping the thread to check for updates.




I have a dozen sawdust bags looking like popcorn and starting to brown. I have more colonizing, and some grain jars going. All different varieties. I don’t have them all on grain yet, but almost!


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28477011 - 09/20/23 07:47 PM (4 months, 5 days ago)



Eight more onto sawdust!


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28477219 - 09/20/23 09:53 PM (4 months, 5 days ago)

Hell yeah. I can't wait to see some fruits!


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Offlinevolleyshroom
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Re: 34 Aloha shiitake cultures [Re: Dragonaut]
    #28477736 - 09/21/23 02:42 PM (4 months, 5 days ago)

This is a really cool project! How did that original group buy work? I'm a newer cultivator and it's encouraging to see group projects that allow for access to such interesting cultures.


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OfflineRedCapRandy
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28516741 - 10/24/23 03:34 PM (3 months, 3 days ago)

This seems like an amazing thing going on here. I am very new but would love to help out and give some a try if you would have me. I really am enjoying the enthusiasm so many in this field are to spread what they can to keep them going. Thank you for your consideration.


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Re: 34 Aloha shiitake cultures [Re: RedCapRandy] * 1
    #28526887 - 11/02/23 05:19 PM (2 months, 25 days ago)

Little update!

Now that we got some cold weather I put six blocks outside to cold shock overnight. Brought them in today and the are soaking. Tomorrow they will get a few slaps and put in the tent.

I lost on block to some contamination. There is about an inch of metabolites in the bottom of the bag, and the block crumbles when touched.

The six that are still going have browned up really nicely.

Two of the blocks have a few fruit in the bag, both LE1 and QUA GU.

The block of 75, the only variety I’ve grown in the past, is quite light and sounds almost hollow.

There are eight more bags that started later. The browning has started, but they’re not quite ready yet. Update and some pics tomorrow!


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28526981 - 11/02/23 06:55 PM (2 months, 24 days ago)

Nice work! Are you going to try to clone anything, or take prints?


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 1
    #28526989 - 11/02/23 07:07 PM (2 months, 24 days ago)

Quote:

Dragonaut said:
Nice work! Are you going to try to clone anything, or take prints?



I never tried printing shiitake. I like to harvest before they start dropping spore. I’ll clone anything that looks interesting, but I still have plates of all these cultures.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns] * 1
    #28530576 - 11/05/23 12:40 PM (2 months, 22 days ago)

Six blocks in the tent a few days ago when I unbagged them.



LE1 showing a few pins already.



Qua Gu pinning up, too!



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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: Buddaking] * 1
    #28530668 - 11/05/23 02:01 PM (2 months, 22 days ago)

:awepreciation:


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 2
    #28537554 - 11/10/23 05:46 PM (2 months, 16 days ago)

Bad news, two of my blocks have some mold. Could be bad spawn, or a nasty tent. So 75 and DON are moldy just as they start pinning.

Good news, Qua Gu is looking good.



LE1 is looking really nice.



The other two are beginning to pin as well. These are in a tent @ 65 degrees F and 85% RH. I’ll be eating some shiitake tomorrow!


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28537636 - 11/10/23 06:33 PM (2 months, 16 days ago)

:highfive: this is amazing! Nice job.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 2
    #28564256 - 12/01/23 07:14 PM (1 month, 26 days ago)

Eight blocks in the tent. I disinfected it after the poor performance last time, and I think it’s much better now. These two have taken off in the last few days.

AM-1



SX-14


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28564286 - 12/01/23 07:26 PM (1 month, 26 days ago)

Looking good!


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 2
    #28568026 - 12/04/23 09:37 AM (1 month, 24 days ago)

Love the sombrero look of these.



They are the AM-1 shiitakes.



They covered the bottom and grew through the wire mesh shelf.



1 1/2 lbs from a 3 1/2 lbs block.


Edited by RoscoeReturns (12/04/23 09:41 AM)


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InvisibleDragonaut


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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28568030 - 12/04/23 09:39 AM (1 month, 24 days ago)

That's awesome! :awebig:

What is that...233% B.E. ? That good for shiitake?


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 1
    #28574800 - 12/08/23 06:45 PM (1 month, 19 days ago)

I don’t know from BE. I know they tasted great.

A pound of 154



And another of AUG



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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28574918 - 12/08/23 08:08 PM (1 month, 19 days ago)

Nice. I love shiitake. Looks appetizing


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Re: 34 Aloha shiitake cultures [Re: deadmandave]
    #28575183 - 12/08/23 11:44 PM (1 month, 19 days ago)

Damn...they do look tasty. :homerdrool:


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InvisiblePhaethon
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Re: 34 Aloha shiitake cultures [Re: Dragonaut] * 1
    #28597622 - 12/25/23 02:30 PM (1 month, 3 days ago)

Very late to the party, but I'd love to have a few cultures if you're willing. I've fruited a supposed S75 culture a few times. Interested to see whether the S75 from Aloha is any better, and I'd love to try 3782 and any random ones we haven't tested yet. I'm planning to focus on shiitake next year, so it'd be awesome to have a few options to see which one works best in my low tech setup (fruiting two blocks per "monotub" now, and hopefully a tent soon, likely without constant humidification or airflow, which seems like it will work fine for at least some shiitake strains).

I'm amazed at your yield with AM-1, RoscoeReturns!


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OfflineMorePies
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Re: 34 Aloha shiitake cultures [Re: Phaethon] * 1
    #28599093 - 12/26/23 08:55 PM (1 month, 1 day ago)

For whatever reason, I stopped by the shroomery and saw this thread. As a commercial grower, I got my start into learning here and it really makes me smile to see this piece of mushroom genetic history being revived. I sent lipa a msg, I wonder if he will stop by too.

Thank you.


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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28599377 - 12/27/23 04:04 AM (1 month, 1 day ago)

holy Shitake


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Offlineflugelizor
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Re: 34 Aloha shiitake cultures [Re: wtfcrazymofo] * 2
    #28604680 - 12/31/23 02:37 PM (28 days, 2 hours ago)

Hmm,
A blast from the past. (was it really 12 years ago? :ahahaha:)
I was a part of this group buy. I was one of the few who actually grew out my assigned strains.
https://www.shroomery.org/forums/showflat.php/Number/16800562#16800562

I still have the original packaging and many of the original cultures. (unrefrigerated)
So many cultures! I only made masters and refrigerated 3:
STRAW, 3782, S75

I have more time on my hands, so I will probably buy a flow hood and revive some.


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Offlineflugelizor
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28604682 - 12/31/23 02:38 PM (28 days, 2 hours ago)

Great work Roscoe,
What was your sawdust recipe?


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OfflineRoscoeReturnsS
Crotchety chode man
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Registered: 02/12/18
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Re: 34 Aloha shiitake cultures [Re: flugelizor] * 1
    #28604934 - 12/31/23 05:28 PM (27 days, 23 hours ago)

Quote:

flugelizor said:
Great work Roscoe,
What was your sawdust recipe?




Thanks.

I’m making 3 - 4 pound blocks with 20% fuel pellets, 20% soy hulls, 60% water all by weight.


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