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InvisibleBuddaking
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Registered: 12/18/09
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34 Aloha shiitake cultures * 2
    #28303995 - 05/02/23 07:39 PM (8 months, 22 days ago)

So back in 2011 a Groupe buy of Shiitake cultures from Aloha Medicinals happened & Lipa killed it making everyone involved slants.

Dragonaut was one of the members involved & stored his amazing in their original packaging in the refer.

He intrusted them to me to breath back life.
Finally I am getting 34 to leap off.

Original thread https://www.shroomery.org/forums/showflat.php/Number/14756674#14756674

Will they still fruit ?

Looking for a couple experienced members to sign on to this task of finding out.

Maby 5 to 10 members willing to try out 2 to 5 of each. Sure different fruiting conditions & Temps freezing substrate ect will effect results & vigor so overlap is good.

You will receive all 34 If you wish, just please run a couple of the lesser known along with the 3782, 75 ect.

In the coming weeks I will prepare .5 ml sets of ependorfs but would like this amazing community to help try em & see if they are still viable.

A complete, updated list will be posted this weekend.

Post here if intrested & when ready I will prepare vials. Prob 2 to 3 weeks as they just literally leaped off this am & a clean transfer to a new nutritional plate is needed.

I will scratch out those that did not make the Journey by this weekend.


1 - GL - P3
2 - SAM'S - P19
3- AUG - P4
4 - CS2 - P16
5 - DON - P4
6 - JP2 - P4
7 - UK7 - P4
8 - UK5 - P4
9 - UK9 - P4
10  - HO1 - P4
11 - CA - P5
12 - CS - P19
13 - 2001 - P17
14 - AH FOOK - P14
15 - F - P3
16 - STRAW - P22
17 - U8 - P10
18 - JUMBO - P20
19 - 3782 - P4
20 - 4015 - P15
21 - TL2 - P4
22 - GE2 - P22
23 - ED - P3 Bacterial beyond rescue
24 - PO1 - P4
25 - CS3 - P10
26 - AM1 - P11
27 - UK8 - P4
28 - UK6 - P4 slow going to try again
29 - 7402 - P4
30 - SX14 - P3
31 - A10 - P23
32 - 75 - P14
33 - EN2 - P4
34 - QUA GU - P15
35 - US1 - P4
36 - HUN - P4
37 - LE1 - P4
38 - PL - P5 Bacterial beyond rescue
39 - IS4 - P4
40 - 720 - NOT READY Missing


Edited by Buddaking (05/08/23 08:37 PM)


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InvisibleDragonaut


Registered: 06/24/04
Posts: 6,190
Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28304090 - 05/02/23 08:38 PM (8 months, 22 days ago)

:heart: This is amazing. :awepreciation:


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OfflineSeeker2be
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28304268 - 05/02/23 11:11 PM (8 months, 22 days ago)

Id like to join this. PM Me


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OfflineHomerSimpson
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Registered: 09/03/22
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Re: 34 Aloha shiitake cultures [Re: Seeker2be]
    #28304388 - 05/03/23 04:25 AM (8 months, 22 days ago)

I would try 2 or 3 random cultures. Let me know.  Thx.


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OfflineStromriderM
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Re: 34 Aloha shiitake cultures [Re: HomerSimpson]
    #28304476 - 05/03/23 06:06 AM (8 months, 22 days ago)

Blast from the past. I would think they would have lost some vigor not being in cryo but it will be cool to see. Good luck


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: HomerSimpson]
    #28304477 - 05/03/23 06:07 AM (8 months, 22 days ago)

I could toss my hat in this and run a few strains. 4-5 would be reasonable in the grow space I have.


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OfflineRoscoeReturnsS
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Registered: 02/12/18
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Re: 34 Aloha shiitake cultures [Re: Harry Manbach]
    #28304485 - 05/03/23 06:11 AM (8 months, 22 days ago)

I would love to be a part of this.


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Offlinespitzenkorper
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Registered: 06/01/17
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28308432 - 05/06/23 12:01 AM (8 months, 19 days ago)

Whoa!
All diff shiitakes??
I’ll try a few


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Offlinedyel
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Registered: 10/15/21
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Re: 34 Aloha shiitake cultures [Re: spitzenkorper]
    #28308532 - 05/06/23 02:59 AM (8 months, 19 days ago)

nvm me just here to watch :popcorn:


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InvisibleBuddaking
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Registered: 12/18/09
Posts: 1,775
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Re: 34 Aloha shiitake cultures [Re: dyel] * 1
    #28311909 - 05/08/23 08:06 PM (8 months, 16 days ago)



Got busy. Going to Transfer tomorrow AM.

Updating 1st post on survivors shortly.

Adding my own 3790 along with unknown I call matsc as that was user here I got it from.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28313300 - 05/09/23 07:43 PM (8 months, 15 days ago)

Amazing work.


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InvisibleBuddaking
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns] * 2
    #28327513 - 05/20/23 12:03 PM (8 months, 5 days ago)

OK so I got a little behind on this project but wrapped up the culture aspect this Morning!

4015 & UK6 did not make the cut as if they refuse to show me vigor on multiple agar mixes their is no reason to continue with a lame horse.

I have 5 sets of .5 ependorfs to share & will be back in town early next week.

All cultures are now slanted 4 different ways
1 grain master of each colonizing to knock up 1 HWFP bag & 200 wood dowells.
Fresh plate of each in cold storage to come back to if vigor in my environment at my Temps is achieved indoor.

20 scrub Oaks just fallen & 8k wood dowells ordered for outdoor logs

Dug out the laminator to make a weather resistant lable for each log pile.



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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28328231 - 05/21/23 01:45 AM (8 months, 4 days ago)

Quote:

Buddaking said:
OK so I got a little behind on this project but wrapped up the culture aspect this Morning!

4015 & UK6 did not make the cut as if they refuse to show me vigor on multiple agar mixes their is no reason to continue with a lame horse.

I have 5 sets of .5 ependorfs to share & will be back in town early next week.

All cultures are now slanted 4 different ways
1 grain master of each colonizing to knock up 1 HWFP bag & 200 wood dowells.
Fresh plate of each in cold storage to come back to if vigor in my environment at my Temps is achieved indoor.

20 scrub Oaks just fallen & 8k wood dowells ordered for outdoor logs

Dug out the laminator to make a weather resistant lable for each log pile.





:wat: absolutely AMAZING


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InvisibleBuddaking
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Registered: 12/18/09
Posts: 1,775
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Re: 34 Aloha shiitake cultures [Re: Harry Manbach] * 3
    #28346434 - 06/03/23 10:39 PM (7 months, 21 days ago)

So it looks like they started landing. I will update the 1st post tomorrow with ones that did not make the cut & replacement #'s for cultures added


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InvisibleDirtnapz
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Registered: 02/22/23
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Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28346722 - 06/04/23 07:10 AM (7 months, 21 days ago)

:takingnotes:

Awesome work, this is great!


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InvisibleDragonaut


Registered: 06/24/04
Posts: 6,190
Re: 34 Aloha shiitake cultures [Re: Buddaking]
    #28347598 - 06/04/23 09:29 PM (7 months, 20 days ago)

If I recall correctly, the "missing" 720 culture from the original project was a late arrival, and was not shipped with the rest of the collection. To the best of my knowledge, I had discarded it after some time along with other things stored separately that did not appear to age well. Sorry about that.


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Dragonaut]
    #28354814 - 06/10/23 07:07 PM (7 months, 14 days ago)

Is there some info on each of these? I’ve got about ten or so onto agar, but was wondering if some are better suited to indoor vs log grows. I know there are warm weather strains, but not which is what. I can find no info on the aloha site. Wondering if there is some info out there already, or if we’ll just discover as we go.


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns]
    #28355922 - 06/11/23 05:24 PM (7 months, 14 days ago)

Quote:

RoscoeReturns said:
Is there some info on each of these? I’ve got about ten or so onto agar, but was wondering if some are better suited to indoor vs log grows. I know there are warm weather strains, but not which is what. I can find no info on the aloha site. Wondering if there is some info out there already, or if we’ll just discover as we go.



:whathesaid:
Just got more agar made and started down this rabbit hole myself.. With little results.

Would love to start a few cultures just based on visual assessment, but do run pretty constant grow conditions and would prefer to apply effort to better suited candidates ... Especially before I give a whack at some log stacks outside!


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OfflineRoscoeReturnsS
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Re: 34 Aloha shiitake cultures [Re: Harry Manbach]
    #28366089 - 06/19/23 06:39 PM (7 months, 5 days ago)

Five of these went to grain today. All will be fruited on supplemented sawdust blocks in my tent. I’ll try to get another five to grain next week. Anyone else making any progress?


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OfflineHarry Manbach
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Re: 34 Aloha shiitake cultures [Re: RoscoeReturns] * 1
    #28366634 - 06/20/23 05:25 AM (7 months, 5 days ago)

I'm still on agar with two of them. My plan is to start 2 per week on agar and either slant or liquid culture each variety as they go to grain for longer storage. This way I don't fill all my incubation space and I can have a nice steady fruiting trial down the road, rather than bombarding my grow room all at once.


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