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InvisibleHILLBILLY OUTLAWS
Above And Beyond!
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Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
Re: Kingdom Come [Re: Bigworm]
    #28309090 - 05/06/23 12:37 PM (8 months, 18 days ago)

You having a derby party BigWorm?


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OfflineNFLProof
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Re: Kingdom Come [Re: HILLBILLY OUTLAW]
    #28309258 - 05/06/23 02:45 PM (8 months, 18 days ago)

Landed..woot woot. BK, would mind elaborating when you suggested using two needles to remove. I'm having a hard time grasping the concept. I only have 2 hands lol.


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OfflineTardisTripS
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Registered: 01/09/23
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Re: Kingdom Come [Re: NFLProof]
    #28309328 - 05/06/23 03:42 PM (8 months, 18 days ago)

I was also wondering about this.


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Because everything has its origin
And I come going from place to place from the origin...




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InvisibleBuddaking
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Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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Re: Kingdom Come [Re: TardisTrip]
    #28309360 - 05/06/23 04:24 PM (8 months, 18 days ago)

Open Ependorf & remove a small square with a needle in 1 hand.
Place ependorf down &  slide square off to a new plate using a second tool. Needle, scalpel, xacto ect.
Removing single squares from these tiny ependorfs is a pita & I found needles flame sterilize & cool quickly + fit in no problem.


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OfflineNFLProof
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Re: Kingdom Come [Re: Buddaking]
    #28309394 - 05/06/23 05:22 PM (8 months, 18 days ago)

Quote:

Buddaking said:
Open Ependorf & remove a small square with a needle in 1 hand.
Place ependorf down &  slide square off to a new plate using a second tool. Needle, scalpel, xacto ect.
Removing single squares from these tiny ependorfs is a pita & I found needles flame sterilize & cool quickly + fit in no problem.





Got it. I was imaging holding two needles and the vial at once. Sorry for the confusion. Thank you.


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Yeah, yeah.


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OfflineNFLProof
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Re: Kingdom Come [Re: NFLProof]
    #28309419 - 05/06/23 05:36 PM (8 months, 18 days ago)

One more...any info on Machine Elf?


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Yeah, yeah.


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InvisibleBuddaking
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Re: Kingdom Come [Re: NFLProof] * 4
    #28309436 - 05/06/23 05:54 PM (8 months, 18 days ago)

Peruvian x PEU
Solid stripe good BE
Doesn't spore dump when ripe.


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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: Kingdom Come [Re: HILLBILLY OUTLAW]
    #28310259 - 05/07/23 12:29 PM (8 months, 17 days ago)

Quote:

HILLBILLY OUTLAW said:
You having a derby party BigWorm?



Nah, it was Saturday and I needed to BBQ something with a cold beer in hand while I had music bumping and friends & family around.
Getting the work done today though.


Edited by Bigworm (05/07/23 12:38 PM)


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InvisibleBigwormS
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Registered: 03/19/22
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Re: Kingdom Come [Re: NFLProof]
    #28310260 - 05/07/23 12:31 PM (8 months, 17 days ago)

Quote:

NFLProof said:


Got it. I was imaging holding two needles and the vial at once. Sorry for the confusion. Thank you.



Hypodermic chopsticks.


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OfflineTardisTripS
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Registered: 01/09/23
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Re: Kingdom Come [Re: Bigworm]
    #28310266 - 05/07/23 12:43 PM (8 months, 17 days ago)

I got some white water plated up. Waiting on some slants to come in then doing the rest.


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Because everything has its origin
And I come going from place to place from the origin...




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InvisibleBigwormS
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Re: Kingdom Come [Re: TardisTrip]
    #28310269 - 05/07/23 12:48 PM (8 months, 17 days ago)

Besides plating these I have to get my outdoor ps.cyan bed going today. Only doing a 3ft x 3ft patch but it should be enough for me.


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InvisibleSlugWorthx1S
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Registered: 03/16/20
Posts: 219
Re: Kingdom Come [Re: NFLProof]
    #28310293 - 05/07/23 01:15 PM (8 months, 17 days ago)

Quote:

NFLProof said:
Quote:

Buddaking said:
Open Ependorf & remove a small square with a needle in 1 hand.
Place ependorf down &  slide square off to a new plate using a second tool. Needle, scalpel, xacto ect.
Removing single squares from these tiny ependorfs is a pita & I found needles flame sterilize & cool quickly + fit in no problem.





Got it. I was imaging holding two needles and the vial at once. Sorry for the confusion. Thank you.




i also found inoculation loops to work perfectly to take out individual squares without damaging

got some plates going


Anyone know if rubbing a plate with a sterile swab would be a viable method of culture storage / sharing?


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InvisibleBigwormS
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Registered: 03/19/22
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Loc: Murica
Re: Kingdom Come [Re: SlugWorthx1] * 1
    #28311125 - 05/08/23 08:00 AM (8 months, 17 days ago)

Forgot to put these up yesterday. Transfers are complete. Next up is putting each one of these into steril distilled water storage cryos.



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OfflineTardisTripS
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Re: Kingdom Come [Re: Bigworm] * 1
    #28311295 - 05/08/23 11:23 AM (8 months, 16 days ago)

You think I should transfer them all on to plates first or would they be good to go on slants right away?


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Because everything has its origin
And I come going from place to place from the origin...




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InvisibleBigwormS
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Re: Kingdom Come [Re: TardisTrip]
    #28311545 - 05/08/23 02:30 PM (8 months, 16 days ago)

Normally I would but these look clean to me so it would be pointless in my opinion. I could be wrong but I have them on agar now as well if something shows up.


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InvisibleBuddaking
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Re: Kingdom Come [Re: Bigworm] * 2
    #28311900 - 05/08/23 08:00 PM (8 months, 16 days ago)

Transfers were clean. Only chink in armor could be the cheep chinacom ependorfs.
Personally I put everything on a new plate prior to LC, Grain or being slanted


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OfflineSeaSalt
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Registered: 12/08/21
Posts: 495
Loc: PNW WWA
Last seen: 4 months, 24 days
Re: Kingdom Come [Re: Bigworm] * 1
    #28312027 - 05/08/23 10:06 PM (8 months, 16 days ago)

Quote:

Bigworm said:
Quote:

HILLBILLY OUTLAW said:
You having a derby party BigWorm?



Nah, it was Saturday and I needed to BBQ something with a cold beer in hand while I had music bumping and friends & family around.
Getting the work done today though.



I had a Derby party for you. 15:1 too bad I don't bet.


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OfflineTardisTripS
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Registered: 01/09/23
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Re: Kingdom Come [Re: Buddaking]
    #28312785 - 05/09/23 01:44 PM (8 months, 15 days ago)

Quote:

Buddaking said:
Transfers were clean. Only chink in armor could be the cheep chinacom ependorfs.
Personally I put everything on a new plate prior to LC, Grain or being slanted




Will do!


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Because everything has its origin
And I come going from place to place from the origin...




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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: Kingdom Come [Re: TardisTrip]
    #28318509 - 05/13/23 04:44 PM (8 months, 11 days ago)

Everything is expanding on agar. All but 2 are super clean. The 2 that aren't clean have satellite contam that I'm sure was my fault. I think it was moisture on the lid that dripped on the plate that caused it. The eppendorf containers seem to still be holding up keeping contams out.


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OfflineTardisTripS
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Re: Kingdom Come [Re: Bigworm]
    #28319031 - 05/14/23 03:48 AM (8 months, 11 days ago)

What were the two you had issues with?


--------------------
Because everything has its origin
And I come going from place to place from the origin...




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