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HILLBILLY OUTLAW
Above And Beyond!



Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
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Re: Kingdom Come [Re: Bigworm]
#28309090 - 05/06/23 12:37 PM (8 months, 18 days ago) |
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You having a derby party BigWorm?
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 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 TEAM SPREAD THE LOVE! Smellyhobbit said: Embarrassment and bashfulness are leeches on your ability to learn.
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NFLProof
Sponge



Registered: 05/31/18
Posts: 893
Loc: In the ether
Last seen: 13 hours, 10 minutes
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Landed..woot woot. BK, would mind elaborating when you suggested using two needles to remove. I'm having a hard time grasping the concept. I only have 2 hands lol.
-------------------- Yeah, yeah.
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TardisTrip
Time Lord



Registered: 01/09/23
Posts: 222
Loc: Colorado
Last seen: 18 hours, 45 minutes
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Re: Kingdom Come [Re: NFLProof]
#28309328 - 05/06/23 03:42 PM (8 months, 18 days ago) |
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I was also wondering about this.
-------------------- Because everything has its origin And I come going from place to place from the origin...

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Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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Open Ependorf & remove a small square with a needle in 1 hand. Place ependorf down & slide square off to a new plate using a second tool. Needle, scalpel, xacto ect. Removing single squares from these tiny ependorfs is a pita & I found needles flame sterilize & cool quickly + fit in no problem.
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 AMU Q & A
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NFLProof
Sponge



Registered: 05/31/18
Posts: 893
Loc: In the ether
Last seen: 13 hours, 10 minutes
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Quote:
Buddaking said: Open Ependorf & remove a small square with a needle in 1 hand. Place ependorf down & slide square off to a new plate using a second tool. Needle, scalpel, xacto ect. Removing single squares from these tiny ependorfs is a pita & I found needles flame sterilize & cool quickly + fit in no problem.
Got it. I was imaging holding two needles and the vial at once. Sorry for the confusion. Thank you.
-------------------- Yeah, yeah.
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NFLProof
Sponge



Registered: 05/31/18
Posts: 893
Loc: In the ether
Last seen: 13 hours, 10 minutes
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Re: Kingdom Come [Re: NFLProof]
#28309419 - 05/06/23 05:36 PM (8 months, 18 days ago) |
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One more...any info on Machine Elf?
-------------------- Yeah, yeah.
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Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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Re: Kingdom Come [Re: NFLProof] 4
#28309436 - 05/06/23 05:54 PM (8 months, 18 days ago) |
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Peruvian x PEU Solid stripe good BE Doesn't spore dump when ripe.
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 AMU Q & A
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Quote:
HILLBILLY OUTLAW said: You having a derby party BigWorm?
Nah, it was Saturday and I needed to BBQ something with a cold beer in hand while I had music bumping and friends & family around. Getting the work done today though.
Edited by Bigworm (05/07/23 12:38 PM)
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Re: Kingdom Come [Re: NFLProof]
#28310260 - 05/07/23 12:31 PM (8 months, 17 days ago) |
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Quote:
NFLProof said:
Got it. I was imaging holding two needles and the vial at once. Sorry for the confusion. Thank you.
Hypodermic chopsticks.
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TardisTrip
Time Lord



Registered: 01/09/23
Posts: 222
Loc: Colorado
Last seen: 18 hours, 45 minutes
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Re: Kingdom Come [Re: Bigworm]
#28310266 - 05/07/23 12:43 PM (8 months, 17 days ago) |
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I got some white water plated up. Waiting on some slants to come in then doing the rest.
-------------------- Because everything has its origin And I come going from place to place from the origin...

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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Besides plating these I have to get my outdoor ps.cyan bed going today. Only doing a 3ft x 3ft patch but it should be enough for me.
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SlugWorthx1


Registered: 03/16/20
Posts: 219
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Re: Kingdom Come [Re: NFLProof]
#28310293 - 05/07/23 01:15 PM (8 months, 17 days ago) |
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Quote:
NFLProof said:
Quote:
Buddaking said: Open Ependorf & remove a small square with a needle in 1 hand. Place ependorf down & slide square off to a new plate using a second tool. Needle, scalpel, xacto ect. Removing single squares from these tiny ependorfs is a pita & I found needles flame sterilize & cool quickly + fit in no problem.
Got it. I was imaging holding two needles and the vial at once. Sorry for the confusion. Thank you.
i also found inoculation loops to work perfectly to take out individual squares without damaging
got some plates going

Anyone know if rubbing a plate with a sterile swab would be a viable method of culture storage / sharing?
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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Forgot to put these up yesterday. Transfers are complete. Next up is putting each one of these into steril distilled water storage cryos.
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TardisTrip
Time Lord



Registered: 01/09/23
Posts: 222
Loc: Colorado
Last seen: 18 hours, 45 minutes
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Re: Kingdom Come [Re: Bigworm] 1
#28311295 - 05/08/23 11:23 AM (8 months, 16 days ago) |
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You think I should transfer them all on to plates first or would they be good to go on slants right away?
-------------------- Because everything has its origin And I come going from place to place from the origin...

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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Normally I would but these look clean to me so it would be pointless in my opinion. I could be wrong but I have them on agar now as well if something shows up.
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Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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Re: Kingdom Come [Re: Bigworm] 2
#28311900 - 05/08/23 08:00 PM (8 months, 16 days ago) |
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Transfers were clean. Only chink in armor could be the cheep chinacom ependorfs. Personally I put everything on a new plate prior to LC, Grain or being slanted
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 AMU Q & A
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SeaSalt



Registered: 12/08/21
Posts: 495
Loc: PNW WWA
Last seen: 4 months, 24 days
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Re: Kingdom Come [Re: Bigworm] 1
#28312027 - 05/08/23 10:06 PM (8 months, 16 days ago) |
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Quote:
Bigworm said:
Quote:
HILLBILLY OUTLAW said: You having a derby party BigWorm?
Nah, it was Saturday and I needed to BBQ something with a cold beer in hand while I had music bumping and friends & family around. Getting the work done today though.
I had a Derby party for you. 15:1 too bad I don't bet.
-------------------- Avatar by Sol_Exposure
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TardisTrip
Time Lord



Registered: 01/09/23
Posts: 222
Loc: Colorado
Last seen: 18 hours, 45 minutes
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Quote:
Buddaking said: Transfers were clean. Only chink in armor could be the cheep chinacom ependorfs. Personally I put everything on a new plate prior to LC, Grain or being slanted
Will do!
-------------------- Because everything has its origin And I come going from place to place from the origin...

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Bigworm



Registered: 03/19/22
Posts: 1,868
Loc: Murica
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Everything is expanding on agar. All but 2 are super clean. The 2 that aren't clean have satellite contam that I'm sure was my fault. I think it was moisture on the lid that dripped on the plate that caused it. The eppendorf containers seem to still be holding up keeping contams out.
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TardisTrip
Time Lord



Registered: 01/09/23
Posts: 222
Loc: Colorado
Last seen: 18 hours, 45 minutes
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Re: Kingdom Come [Re: Bigworm]
#28319031 - 05/14/23 03:48 AM (8 months, 11 days ago) |
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What were the two you had issues with?
-------------------- Because everything has its origin And I come going from place to place from the origin...

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