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johnnytheswede
Knowledge seeker



Registered: 08/29/14
Posts: 436
Loc: Sweden
Last seen: 7 months, 11 days
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Restarting mycelium from an old jar
#28296399 - 04/26/23 03:54 PM (8 months, 28 days ago) |
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Hi Forum,
I am about to try to restart a culture that has been sitting in a jar for about 5 years I think ... it is originally a fully colonized quart jar of rye.
It has shrunk through the years and created a lot of yellow liquid but I am hoping that the insides of the lump will be ok.
My thought is that I will extract the lump from the jar and take pieces from deep within the lump and put onto agar in front of the flow hood.
What do you think about this?
Kindest regards Johnny
-------------------- Just learning to cultivate mushrooms, immensely impressed by Paul Stamets ("Pair up with Fungi!") and the knowledge and helpfulness at The Shroomery. Concentrating on Gourmet and Medicinal Mushrooms: Reishi, Shiitake, Garden Giant, Lion's Mane, Turkey Tail. Acronyms (for noobs like me): http://www.shroomery.org/forums/showflat.php/Number/5875173#Post5875173 Have opted out of rating system because of personal reasons, read my bio if you are interested in why. (I opted out before receiving or giving any ratings, meaning no offense to anyone.)
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ChRnZN
Din of Doom


Registered: 12/21/08
Posts: 6,265
Loc: ADK
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How do you know the lump isn't just a bacterial blob that killed the mycelium sometime in the past 5 years? Open your jar while wearing a mask and gloves, make sure it isn't something dangerous.
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johnnytheswede
Knowledge seeker



Registered: 08/29/14
Posts: 436
Loc: Sweden
Last seen: 7 months, 11 days
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Re: Restarting mycelium from an old jar [Re: ChRnZN]
#28296412 - 04/26/23 04:05 PM (8 months, 28 days ago) |
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Thank you for your response. My thinking is that I will transfer pieces onto agar and if I from this point on can observe mycelial growth rather than bacterial growth, that would indicate that is is ok.
However, I do become a bit worried when you seem to imply that it might be dangerous ... could it be dangerous somehow?
Kind regards Johnny
-------------------- Just learning to cultivate mushrooms, immensely impressed by Paul Stamets ("Pair up with Fungi!") and the knowledge and helpfulness at The Shroomery. Concentrating on Gourmet and Medicinal Mushrooms: Reishi, Shiitake, Garden Giant, Lion's Mane, Turkey Tail. Acronyms (for noobs like me): http://www.shroomery.org/forums/showflat.php/Number/5875173#Post5875173 Have opted out of rating system because of personal reasons, read my bio if you are interested in why. (I opted out before receiving or giving any ratings, meaning no offense to anyone.)
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ChRnZN
Din of Doom


Registered: 12/21/08
Posts: 6,265
Loc: ADK
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Think about it. Five years is a long time. Imagine what could grow in an undisturbed jar in 5 years. Just about anything.
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dyel



Registered: 10/15/21
Posts: 920
Last seen: 16 hours, 24 minutes
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Re: Restarting mycelium from an old jar [Re: ChRnZN]
#28297370 - 04/27/23 09:37 AM (8 months, 27 days ago) |
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Quote:
johnnytheswede said: Thank you for your response. My thinking is that I will transfer pieces onto agar and if I from this point on can observe mycelial growth rather than bacterial growth, that would indicate that is is ok.
However, I do become a bit worried when you seem to imply that it might be dangerous ... could it be dangerous somehow?
Kind regards Johnny
go for it, you should be using sterile procedure while working with agar anyways
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johnnytheswede
Knowledge seeker



Registered: 08/29/14
Posts: 436
Loc: Sweden
Last seen: 7 months, 11 days
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Re: Restarting mycelium from an old jar [Re: dyel]
#28298699 - 04/28/23 09:37 AM (8 months, 26 days ago) |
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Thank you guys, I will think about this a bit and then make a decision. If I go ahead with this, I will of course post an update describing my experiences.
Kindest regards Johnny
-------------------- Just learning to cultivate mushrooms, immensely impressed by Paul Stamets ("Pair up with Fungi!") and the knowledge and helpfulness at The Shroomery. Concentrating on Gourmet and Medicinal Mushrooms: Reishi, Shiitake, Garden Giant, Lion's Mane, Turkey Tail. Acronyms (for noobs like me): http://www.shroomery.org/forums/showflat.php/Number/5875173#Post5875173 Have opted out of rating system because of personal reasons, read my bio if you are interested in why. (I opted out before receiving or giving any ratings, meaning no offense to anyone.)
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