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MushMan303
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Registered: 01/26/23
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Fruiting from my cloned rhizo agar into cv sub
#28298180 - 04/27/23 10:11 PM (1 year, 8 months ago) |
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https://files.shroomery.org/files/23-17/265502941-IMG_4814.jpg
Hey y’all I cloned a fine one from my first flush I did from spores last go. I grew two plates off it with rhizo agar and put into multi grain then to Coco Coir, Vermiculite, Hydrated Lime, Gypsum, ph buffers, ph stabilizers, mineral water and a few other choice ingredients. Is this normal for rhizomorphic mycelium out of casing layer?
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johnukguy
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Re: Fruiting from my cloned rhizo agar into cv sub [Re: MushMan303]
#28298198 - 04/27/23 10:24 PM (1 year, 8 months ago) |
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Welcome. First off, good to know that you're using agar. That's refreshing here and a solid way to do things. The mycelium may be a little on the dry side if the container is fully open like that. I am wondering why that container is on top of perlite? Perlite is usually for cakes, which aren't in a separate container as I understand it with PF Tek.
Also, why all the additives? Gypsum for example doesn't do anything for mycelium and all the ph buffers, lime, mineral water and so on are also unnecessary, especially if you just use straight coir, or coir and vermiculite substrate. The substrate for cubes is primarily a water reservoir, not really food for the mycelium, so additives do little to nothing.
A shoebox setup, with coir and a non airtight lid on the shoebox, would likely be a better option. The fewer additives, the less contamination vectors and the less potential complications. Is this from a Tek?
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Way
The


Registered: 01/14/23
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Re: Fruiting from my cloned rhizo agar into cv sub [Re: johnukguy] 1
#28298213 - 04/27/23 10:48 PM (1 year, 8 months ago) |
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 Aerial mycelium is due to lack of fresh air. Looks fairly normal and like it's still colonizing.
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MushMan303
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Re: Fruiting from my cloned rhizo agar into cv sub [Re: johnukguy]
#28298220 - 04/27/23 10:54 PM (1 year, 8 months ago) |
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I live in a dry climate and I am using a two tub setup. The bottom has water and an aquarium heater. The top tub is a regular 6 whole tub with perlite and some water to keep it at 75-90% humidity. The top tub is my stage for fruiting. I colonized a multi grain jay perfect from agar and a couple other ways that aren’t ready yet. The first that colonized was the grain jay so I mixed it and topped it off with a substrate that was pre bought and sterilized. I left that sit for 5 days in the container and lid on for it to colonize then I put it into the mono tub for fruiting as it is very dry here. My temp is 77 steady and 80% humidity. The warm water in the tub under the top stays constant temp and heats the water in the perlite to create a high RH, I just never saw the mycelium fluff out on the ends like this.

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johnukguy
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Re: Fruiting from my cloned rhizo agar into cv sub [Re: MushMan303] 1
#28298228 - 04/27/23 11:04 PM (1 year, 8 months ago) |
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I would say to forget about relative humidity. It's not that important, if at all, so long as the substrate itself is at, or near field capacity, and the surface of the substrate when around full colonization, has lots of tiny beads of water on it. The evaporation of those tiny water beads from the substrate surface is a major pinning trigger. What's going on even a couple of inches away from the surface isn't really a factor.
There appears to me here to be a kind of confusion between shoebox type growing and using a water tub for something like PF Tek. I would recommend doing either one or the other to start off with, as opposed to trying to do both. So ditch the water heater and perlite setup.
Since the substrate in this case is already colonized, I'd say to take the container out of the water tub setup, put a non airtight lid on it, don't fan it, mist it if it dries up and there are no little water beads on the surface. Let it have a little light, ambient light for example is fine. Going forward, I'd say to follow a trusted Tek, use plain coir, or coir and verm for cube substrate, and leave out all of the additives.
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“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
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MushMan303
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Registered: 01/26/23
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Re: Fruiting from my cloned rhizo agar into cv sub [Re: johnukguy]
#28298245 - 04/27/23 11:24 PM (1 year, 8 months ago) |
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Thanks for the input. The last time I used this same setup with a 5lb all in one bag that colonized and pinned, then I removed it from the bag and put it in this setup and it went off for 5 flushes. I will take this out and cover it until it pins then take it out of container and put it on foil back in tub. So it is probably too much moisture and too little air for the shoebox/container I put in there.
Edited by MushMan303 (04/27/23 11:26 PM)
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johnukguy
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Re: Fruiting from my cloned rhizo agar into cv sub [Re: MushMan303]
#28298250 - 04/27/23 11:28 PM (1 year, 8 months ago) |
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It's not a good idea to keep disturbing the mycelium. If you're going to do it as a shoebox type grow, then do that. If you're going to do it as a PF Tek type grow, then stick with that. Just don't keep mixing the two.
-------------------- How to post pictures to shroomery TEK
Shroomery Trusted Cultivator And Member YouTube Channels.
“Evey Hammond: Who are you?
V: Who? Who is but the form following the function of what and what I am is a man in a mask.
Evey Hammond: Well I can see that.
V: Of course you can. I'm not questioning your powers of observation I'm merely remarking upon the paradox of asking a masked man who he is”
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MushMan303
Stranger

Registered: 01/26/23
Posts: 4
Last seen: 1 year, 3 months
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Re: Fruiting from my cloned rhizo agar into cv sub [Re: MushMan303]
#28370621 - 06/22/23 08:11 PM (1 year, 6 months ago) |
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Came out good continued this way the cake did 11 flushes now have 5 going. This is the 2nd flush.
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