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OfflineDendrocopos
Latin woodpecker
I'm a teapot


Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 8 months
Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
    #27790843 - 05/24/22 04:17 PM (1 year, 8 months ago)

Quote:

Crackatoa said:
Quote:

holybrown101 said:
no filter you say....
I'm knocking up 2 jars today but with jello, i have agar but wanted to try something different. Will post results. CANT WAIT



:llamastare::pondering:




:lol:


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OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
Re: SEMI-SOLID LIQUID CULTURE [Re: karri0n]
    #27791298 - 05/24/22 09:54 PM (1 year, 8 months ago)

I appreciate the quality reply. All interesting real life experience that will definitely help. I’ve talked to a few people who absolutely swear by peptone, one said they feel like they grew incorrectly for years without it. IDK.

Do you use a magnetic stirrer?


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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
Last seen: 1 day, 1 hour
Trusted Cultivator
Re: SEMI-SOLID LIQUID CULTURE [Re: CatsLoveHouseMusic]
    #27791311 - 05/24/22 10:05 PM (1 year, 8 months ago)

I do, and you will with SSLC, IMO.


--------------------

Crack's Tek's and Shit



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Offlinekarri0n
Mind Traveller
Male User Gallery


Registered: 08/29/20
Posts: 698
Last seen: 10 days, 5 hours
Re: SEMI-SOLID LIQUID CULTURE [Re: Crackatoa]
    #27793674 - 05/26/22 12:46 PM (1 year, 8 months ago)

I've never used an electric stirrer. I've only just started using SSLC, but I can say definitively, a very wide range of nutrients and nutrient levels work great with LC.




I've got psilocybe cubensis, Pleurotus ostreatus(oyster), Coprinus Comatus(shaggy mane), panaeolus Bisporus, and psilocybe natalensis on this shelf.

Nutrients in most of them are different, and different ratios from invisible to slight sediment to opaque(before colonization). The four that show cloudiness, starting three from the left, in this photo, are the SSLC.

Diluted Corn boil water, blackstrap molasses between 0.5g and 10g, WBS + Oat flour fully saturated water(the opaque one in the middle), half teaspoon of same flour water to 250 ml water, and LME at 0.1 to 0.2 % are all shown. Each colonized fine and most of them have been grown to fruits already.

I'm still trying to find a favorite nutrient and I am leaning toward flour water made from the grain you plan on using due to the recovery speed. I love the clarity of blackstrap molasses though. No sediment ever.


--------------------

Panaeolus Bisporus


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OfflineCatsLoveHouseMusic
SpeakerFreaker
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Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
Re: SEMI-SOLID LIQUID CULTURE [Re: karri0n]
    #27794341 - 05/26/22 08:31 PM (1 year, 8 months ago)

I have LME and was thinking about using LME/Peptone/water in quarts and try some pints. Going to try some SSLC and regular.

I’m also going to try some jars with quality SHIPs/Syringe Filters, and some unmodded in 500ml media bottles.

Seems like people have so many different opinions, it’s tough to know where a good starting point is. I have to order the peptone still though, I also have PP5 plastic lids.


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Invisiblenix21
Stranger
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Registered: 05/29/18
Posts: 622
Re: SEMI-SOLID LIQUID CULTURE [Re: CatsLoveHouseMusic]
    #27794498 - 05/26/22 11:10 PM (1 year, 8 months ago)

Did anyone tried store sslc in fridge for few months? Any inputs how it acts after extended period of time in cold storage?


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InvisibleSwabMarleyS
Twisted Metal Head
Male


Registered: 12/07/20
Posts: 1,553
Loc: Drunken Stupor
Re: SEMI-SOLID LIQUID CULTURE [Re: nix21] * 1
    #27931607 - 09/03/22 01:57 AM (1 year, 5 months ago)

Bump!

Yes these store fine in the fridge just like a normal LC. Got one from 3-4 months ago that recovered on grain like it was freshly made!


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OfflinePadaone
Mycophage

Registered: 07/03/22
Posts: 66
Last seen: 1 year, 3 days
Re: SEMI-SOLID LIQUID CULTURE [Re: SwabMarley] * 1
    #27942556 - 09/09/22 07:43 PM (1 year, 5 months ago)

Great tek,

Gave it a go and seemed like I had clean growth. Nocc'd up some jars and its growth looks a little different to what I'm used to though looks similar to pics in OP, what do you guys think?



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OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
Last seen: 1 day, 1 hour
Trusted Cultivator
Re: SEMI-SOLID LIQUID CULTURE [Re: Padaone]
    #27942574 - 09/09/22 07:58 PM (1 year, 5 months ago)

Quote:

Padaone said:
Great tek,

Gave it a go and seemed like I had clean growth. Nocc'd up some jars and its growth looks a little different to what I'm used to though looks similar to pics in OP, what do you guys think?





:asianofapproval:


--------------------

Crack's Tek's and Shit



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OfflineCob
Ovoid fanboy
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Registered: 07/01/22
Posts: 444
Loc: East Coast, USA
Last seen: 8 months, 29 days
Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27942782 - 09/09/22 11:13 PM (1 year, 5 months ago)

Awesome Tek, I just wanted to post to keep it in my threads. Looks like I could use my tall pint jars for this SSLC with unmodified plastic lids I have. The tall pints don't work so well for me doing PF Tek. This looks like a great way to make use of them!


--------------------


****FUGGIN' FROGZ****

Trade List


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Offlinejakemp
Stranger


Registered: 09/11/22
Posts: 73
Last seen: 15 days, 19 hours
Re: SEMI-SOLID LIQUID CULTURE [Re: Cob]
    #27956171 - 09/18/22 02:31 PM (1 year, 4 months ago)

This seems awesome! Will try this when i get to LC stage!


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OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,317
Last seen: 3 hours, 42 minutes
Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #27976556 - 10/01/22 12:10 PM (1 year, 4 months ago)

Good contributions my guy 👍


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OfflineSymPlayTon
Noobinstien

Registered: 09/03/22
Posts: 420 420 Posts!
Loc: In a state of confusion
Last seen: 4 months, 4 days
Re: SEMI-SOLID LIQUID CULTURE [Re: Screwup]
    #27976604 - 10/01/22 12:35 PM (1 year, 4 months ago)

I made a batch yesterday.  Put a magnetic stir rod in it. 

It just sets up like a jello, but the stir rod was able to make it flow like a liquid still.  It is an interesting LC. 

I think an advantage it might have over Nirmal LC is the ability to fix air bubbles into suspension.  This creates a massive surface area, and gas pocket availability. 

I have yet to inoculate it still, but I will take pictures later and keep you updated.


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Offlinejakemp
Stranger


Registered: 09/11/22
Posts: 73
Last seen: 15 days, 19 hours
Re: SEMI-SOLID LIQUID CULTURE [Re: SymPlayTon]
    #27976619 - 10/01/22 12:42 PM (1 year, 4 months ago)

Let us know for sure! I am gonna try this within the next week or so. waiting for some agar plates to grow out.


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OfflineRusty2096
rah rah raw in Lady gaga
I'm a teapot User Gallery


Registered: 08/23/22
Posts: 4,946
Loc: 🌌
Last seen: 9 days, 22 hours
Re: SEMI-SOLID LIQUID CULTURE [Re: SymPlayTon]
    #27977040 - 10/01/22 05:44 PM (1 year, 4 months ago)

Quote:

Cob said:
Awesome Tek, I just wanted to post to keep it in my threads. Looks like I could use my tall pint jars for this SSLC with unmodified plastic lids I have. The tall pints don't work so well for me doing PF Tek. This looks like a great way to make use of them!




Scroll all the way to the bottom. On the left. Click :wink:



Quote:

SymPlayTon said:
I made a batch yesterday.  Put a magnetic stir rod in it. 

It just sets up like a jello, but the stir rod was able to make it flow like a liquid still.  It is an interesting LC. 

I think an advantage it might have over Nirmal LC is the ability to fix air bubbles into suspension.  This creates a massive surface area, and gas pocket availability. 

I have yet to inoculate it still, but I will take pictures later and keep you updated.




Try with a little less agar maybe. I don't have a magnetic stirrer so I just dropped 3 pieces of broken glass in mine and I can easily hand swirl it.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)


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OfflineSymPlayTon
Noobinstien

Registered: 09/03/22
Posts: 420 420 Posts!
Loc: In a state of confusion
Last seen: 4 months, 4 days
Re: SEMI-SOLID LIQUID CULTURE [Re: Rusty2096]
    #27977095 - 10/01/22 06:33 PM (1 year, 4 months ago)

Quote:

Rusty2096 said:
Quote:

Cob said:
Awesome Tek, I just wanted to post to keep it in my threads. Looks like I could use my tall pint jars for this SSLC with unmodified plastic lids I have. The tall pints don't work so well for me doing PF Tek. This looks like a great way to make use of them!




Scroll all the way to the bottom. On the left. Click :wink:



Quote:

SymPlayTon said:
I made a batch yesterday.  Put a magnetic stir rod in it. 

It just sets up like a jello, but the stir rod was able to make it flow like a liquid still.  It is an interesting LC. 

I think an advantage it might have over Nirmal LC is the ability to fix air bubbles into suspension.  This creates a massive surface area, and gas pocket availability. 

I have yet to inoculate it still, but I will take pictures later and keep you updated.




Try with a little less agar maybe. I don't have a magnetic stirrer so I just dropped 3 pieces of broken glass in mine and I can easily hand swirl it.





I think what happened with mine is evaporation. 

I started with .5g for 250ml.  It went down to about 210ml during PC. That is a big difference in the ratio at that volume.


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Offlinejakemp
Stranger


Registered: 09/11/22
Posts: 73
Last seen: 15 days, 19 hours
Re: SEMI-SOLID LIQUID CULTURE [Re: SymPlayTon]
    #27977396 - 10/02/22 12:25 AM (1 year, 4 months ago)

Make sure your cap to the container is lose.. I i lost about 100mls on my last agar run.. I loosened the cap a little more on my second and didnt lose any!
(could of got lucky i guess..lol)


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Offlinenakadash1
Stranger
Registered: 11/02/22
Posts: 156
Last seen: 3 months, 1 day
Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #28269606 - 04/09/23 06:59 AM (10 months, 3 days ago)

Are these low nutrient concentrations (like 0.1%) something specific to this agar LC technique or is it just a matter of preference? I used to use 2-4% dextrose/LME, which I recently reduced to 1.5% which seemed like a substantial decrease already. Going to 0.1% is so far from what I'm currently using that I can't help but to wonder how the results could be similar. Is it wrong to assume that there must be some diminished amount of mycelium after full colonization between using a 0.1% nutrient solution vs a 1.5% solution? Even if its not 15 times less mycelium, how can a similar amount of mycelium colonize the jar with so much less available nutrients?

Also, the comment about adding 0.5% agar to the LC causing it to solidify (and others reporting solidifying LC with similarly low concentrations) is very confusing to me. I make my plates with 1.8% to 2% agar; and when I tried concentrations between 1.6-1.7%, the agar was very jello-like and was difficult to cut and especially hard to transfer because I wasn't able to poke it. Instead I basically had to try to use the scalpel as a spoon and pick the soft jelly-like wedge up. I just can't imagine how there could be agar that is so different from other types that it would cause LC to solidify when used at 0.5%. :confused:


--------------------
#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold


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OfflinePandaskis
Eating Bamboo
Male User Gallery


Registered: 03/14/23
Posts: 1,869
Last seen: 1 month, 21 days
Re: SEMI-SOLID LIQUID CULTURE [Re: natedawgnow]
    #28269618 - 04/09/23 07:15 AM (10 months, 3 days ago)

I wonder if you can sub the agar for gelatin, that way you can make it more solid initially and as the mycelium eats the gelatin it will become more watery. Wonder if its possible to spot contam in its more solid form before the mycelium turns it watery.

Just some crazy thoughts haha


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Invisiblemilkboy
Child
I'm a teapot User Gallery


Registered: 05/01/21
Posts: 2,314
Re: SEMI-SOLID LIQUID CULTURE [Re: nakadash1]
    #28269629 - 04/09/23 07:27 AM (10 months, 3 days ago)

Quote:

nakadash1 said:
Are these low nutrient concentrations (like 0.1%) something specific to this agar LC technique or is it just a matter of preference? I used to use 2-4% dextrose/LME, which I recently reduced to 1.5% which seemed like a substantial decrease already. Going to 0.1% is so far from what I'm currently using that I can't help but to wonder how the results could be similar. Is it wrong to assume that there must be some diminished amount of mycelium after full colonization between using a 0.1% nutrient solution vs a 1.5% solution? Even if its not 15 times less mycelium, how can a similar amount of mycelium colonize the jar with so much less available nutrients?

Also, the comment about adding 0.5% agar to the LC causing it to solidify (and others reporting solidifying LC with similarly low concentrations) is very confusing to me. I make my plates with 1.8% to 2% agar; and when I tried concentrations between 1.6-1.7%, the agar was very jello-like and was difficult to cut and especially hard to transfer because I wasn't able to poke it. Instead I basically had to try to use the scalpel as a spoon and pick the soft jelly-like wedge up. I just can't imagine how there could be agar that is so different from other types that it would cause LC to solidify when used at 0.5%. :confused:




Nutes in LC is very much a preference thing. I find high nutritional LC becomes very.... thick over time, this with the addition of agar in a SSLC can cause some damn thick solutions if sitting for a while which is not my preference.

Feel free to try .5% agar but you wont like the results, .1% works great, .2% felt unnecessary thicker to me. I also believe agar strength can vary a little by brand.


--------------------
Gummies by milkboy
Milkboys Public Display of Affection

"Vires acquirit eundo"



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