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OfflineTheHairPillow
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Registered: 06/25/04
Posts: 2
Loc: Floating
Last seen: 12 years, 5 months
Containers?
    #2826768 - 06/25/04 04:43 AM (12 years, 5 months ago)

Afoaf has been thinking about the possibly of using ziploc containers (microwave safe) for syringe innoculation.  The idea is to cut a 1"X1" hole in the lid and with clean hands put a fresh big band-aid over it.  Then microwave WBS(or other substrate)  with the lid on for 3-4 minutes, allow to cool.  Then, using clean techniques, syringe inject spore/culture and follow by covering syringe hole(s) with small round band-aid(s).  Would the baid-aids work to keep contams out but allow for gas exchange?  If so,  would this mean goodbye to the pressure cooker?  If a band-aid would work in this situation, one could techinically make their own spawn bags no?  Either way if the idea would work, it would be simple to case..No switching containers.  This foaf also thinks after innoculation it might not be a bad idea to rub the outside of the band-aid with h2o2 or alcohol.  Anyone here experimented with this tek? Is polyfill microwave safe?  If so, replace band-aids with polyfill in said ziPtek? Looking for comments :smile:

Pillow


--------------------
"My boys get that syrup in them and they get all antsy in their pantsy"


Edited by TheHairPillow (06/25/04 05:46 AM)


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OfflineAnnoA
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Re: Containers? [Re: TheHairPillow]
    #2826882 - 06/25/04 06:56 AM (12 years, 5 months ago)

You can not effectively sterilize any substrates by microwaving them for only a few minutes. Particulary not grains. And when you microwave it for a longer time, you get problems with the water content.


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OfflineTheHairPillow
jazzer
Registered: 06/25/04
Posts: 2
Loc: Floating
Last seen: 12 years, 5 months
Re: Containers? [Re: Anno]
    #2827006 - 06/25/04 07:53 AM (12 years, 5 months ago)

Anno, is there any way to compensate with more water and go somewhere like 8 minutes?

pillow


--------------------
"My boys get that syrup in them and they get all antsy in their pantsy"


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OfflineAnnoA
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Re: Containers? [Re: TheHairPillow]
    #2827073 - 06/25/04 08:28 AM (12 years, 5 months ago)

You can try, still, you won't have much luck with grains and microwave.


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Offlinemycoguy
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Registered: 03/25/04
Posts: 874
Loc: PNW
Last seen: 6 years, 1 month
Re: Containers? [Re: TheHairPillow]
    #2828164 - 06/25/04 03:12 PM (12 years, 5 months ago)

there's been some discussions on "fractional sterilization" in the micro, but just go out and find a PC at a thrift store. I've found many there. Just make sure it has the weight, and a healthy seal.
They're cheap at thrift stores man.


--------------------

(and no, that's not me in the avatar)
Yahoo! Pacific Northwest Mycology Group


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