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Offline3.A.M
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Psilocybin levels tested using a spectrometer ( spectrophotometer )
    #28261477 - 04/04/23 03:45 AM (1 year, 1 month ago)

I have a good friend that owns this equipment for their day to day work and would like to test psilocybin levels of local native mushrooms for research purposes but cannot find any information on techniques that may be viable, they apparently came across a paper years ago but has had no luck tracking it down and neither of us can find anything relevant using the search engine, any help would be greatly appreciated, thank you!


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InvisibleBaba Yaga
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: 3.A.M]
    #28261501 - 04/04/23 04:30 AM (1 year, 1 month ago)


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Offline3.A.M
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: Baba Yaga]
    #28261651 - 04/04/23 07:48 AM (1 year, 1 month ago)

Dang Baba, I reckon you’re the first responder to 99% of my questions, definitely earning that badge!
Congratulations btw :smile:
I think a mass spectrometer is a step up from what they’re using and their main issue (from what I could gather as most was over my head) was stabilising the sample for consistent analysis as oxidisation, light, temperature and humidity along with other variables were messing with results and giving inconsistent data, they know it’s possible but can’t figure out how.
They asked if I could give this stab in the dark on their behalf in the hope that some random big brain might have the information handy or point us in the direction of someone who will.


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InvisibleTung
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: 3.A.M]
    #28269967 - 04/09/23 01:06 PM (1 year, 1 month ago)

I think these papers would be useful in this discussion:
https://academic.oup.com/jaoac/article/86/6/1124/5657041
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3644759/
The TLDR is that they use some color-changing indicator (BCG) which will have a very strong absorbance at a certain wavelength after reacting with the active ingredient they want to find the concentration of. Doing this with psilocybin or any active mushroom alkaloid seems hard since the only commonly available indicator I know that would react with psilocybin is Ehrlich, which would also react and change color in the presence of other non-active indoles in the mushroom. This would artificially boost your concentration results by some unknown amount and make the data essentially useless. Unless someone knows of some publicly available reagent that would only really react with the active alkaloids it seems like the data you'd get wouldn't be very accurate. My friend is building a UV-Vis photo spectrometer soon, so if anyone knows about a chemical like that I would love to hear about it and do some experiments on the accuracy compared to a proper HPLC. :eek:

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InvisibleRatThing
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: 3.A.M]
    #28271904 - 04/10/23 09:04 PM (1 year, 1 month ago)

Tryptamine Potency Analysis of Psilocybe Mushrooms ✚ Further Analysis; Microdosing Ranges
Fall [Autumn] 2022 Hyphae Cup Distributions
Oakland Hyphae LLC [Jan 2023]

https://www.reddit.com/r/PsychedelicStudies/comments/12er395/tryptamine_potency_analysis_of_psilocybe/


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Offline3.A.M
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: Tung]
    #28275410 - 04/13/23 05:40 AM (1 year, 1 month ago)

Cheers for the replies Tung and ratthing, I’ll pass this information along, can’t imagine it’ll be too long before someone comes up with a simple quantity/qualitative method of testing this, I hope.


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OfflineAlan RockefellerM
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: 3.A.M]
    #28278131 - 04/14/23 10:03 PM (1 year, 1 month ago)

Quote:

3.A.M said:
Cheers for the replies Tung and ratthing, I’ll pass this information along, can’t imagine it’ll be too long before someone comes up with a simple quantity/qualitative method of testing this, I hope.





I think if spectrophotometers could measure psilocybin accurately, people would be using them for this regularly.  They are so cheap and fast compared to HPLC. 

People use HPLC for this though - there must be a good reason.

Maybe because the sample needs to be purified to make the spectrophotometry meaningful - so you might as well run it through a chromatography column.

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Offline3.A.M
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: Alan Rockefeller]
    #28278331 - 04/15/23 04:13 AM (1 year, 1 month ago)

Cheers man, more information I can add to the pile :laugh:


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Invisiblemyc_ousin_vinny
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Re: Psilocybin levels tested using a spectrometer ( spectrophotometer ) [Re: 3.A.M]
    #28281101 - 04/16/23 07:51 PM (1 year, 1 month ago)

Saw this in a thread on Psilomethoxin. Reminded me of this discussion.

https://chemrxiv.org/engage/chemrxiv/article-details/64358de9736114c96352edf9


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