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InvisibleJHOVA
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: JHOVA]
    #28163203 - 01/29/23 04:43 PM (1 year, 17 days ago)

What's the best way to get rid of the cloudy film that builds up on these?

Here's some shots of whyte gold on on pasty pda with agave T1. It really is hard to see if theres any contams going on without opening em up and doing a full sab session. Time for glass petris.



Edited by JHOVA (01/30/23 06:51 PM)


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Offlineexamine
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: jay.ach] * 4
    #28169889 - 02/02/23 08:45 PM (1 year, 13 days ago)

I love the HG plates and owe Alien a debt of gratitude for pioneering this approach. I want to try to pay it forward a little by adding my experiences to the thread.

I have been sourcing most of mine from the wholesalesuppliesplus link shared earlier in the thread. The ones from that source are a bit less clear than the ones from the sources Alien linked in the first post, but they're good enough for my needs and a better value (96 containers for $57 shipped).

I have an alternate method for de-fogging the plates that gets me very usable plates much faster and allows me to sterilize more plates at once because I don't need to use outer containers like Alien's nutcracker.  I can comfortably fit at least 75 plates in my AA 25X, and the first 24 plates are clear and ready to use within a few hours with the rest of them ready within a day or two.

The key is to use a heating pad with an adjustable temperature and unlimited heating time (no auto-off safety feature). Here is a non-affiliate link to the one I use: Pure Enrichment PureRelief XL

Here's a rundown of my method -

After sterilizing, I take the plates out as soon as the PC pressure has dropped naturally, and with the lids still loosened about a quarter turn, I lay them out flat on a wire cooling rack in a rectangle the size of my heating pad. I can fit 24 plates at a time under my heating pad in eight rows of three. I lay the heating pad on top of them and turn it up to the maximum temperature (it can maintain around 140 F at maximum), and I put a towel over the top to help conserve the heat. The idea is that the plates are being heated from the top and have cooling and ventilation from the bottom. I check them after half an hour and make sure there is no remaining condensation on the lids, and I turn down the heat by one notch. Then I wait another 30 minutes and turn down the heat another notch, repeating until the heat is off.  Lastly, I wait awhile longer for everything to fully cool down to room temperature before finally removing the towel and heating pad. At this point the plates are clear and stable and ready to store or use.


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InvisibleFloret
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: examine]
    #28180946 - 02/10/23 01:30 PM (1 year, 5 days ago)

Thanks for sharing this. The condensation was driving me nuts and I considered whether a heating pad would work.


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Offlinesporemaster420
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Floret]
    #28198234 - 02/21/23 09:04 PM (11 months, 18 days ago)

I haven’t tried this, but I have some questions.

β‘  if the top container in a stack is the only one with condensation, why not just put an empty top container there?

β‘‘ has anyone tried putting their moisture filled plates in a big air tight tipper wear container with a bag of damp-rid? (supersized bag of duhumidifying agent of some sort)


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Invisibledowodenum
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: sporemaster420] * 1
    #28198242 - 02/21/23 09:10 PM (11 months, 18 days ago)

Quote:

sporemaster420 said:
I haven’t tried this, but I have some questions.

β‘  if the top container in a stack is the only one with condensation, why not just put an empty top container there?

β‘‘ has anyone tried putting their moisture filled plates in a big air tight tipper wear container with a bag of damp-rid? (supersized bag of duhumidifying agent of some sort)




1. Because the top dish needs some heat capacity (from the agar) for that to work, otherwise it's not much different from empty air.

2. This would dry out the agar, possibly even faster than the condensation droplets themselves.

I will point you here though - but it's not great for no-pours with little structural support: https://www.shroomery.org/forums/showflat.php/Number/27189961


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InvisibleBen Phartin

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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dowodenum]
    #28204050 - 02/25/23 12:37 PM (11 months, 14 days ago)

They are back in stock with prime delivery for $13.99 as of right now if anyone has been waiting on them like I was.

https://www.amazon.com/gp/product/B083HXXB34


Edited by Ben Phartin (02/25/23 01:00 PM)


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Invisibledowodenum
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Ben Phartin]
    #28204067 - 02/25/23 12:46 PM (11 months, 14 days ago)

Quote:

Ben Phartin said:
They are back in stock with prime delivery for $13.99 as of right now if anyone has been waiting on them like I was.

https://www.amazon.com/gp/product/B083HXXB34




Heh they're gone already. Cleaned up the Amazon link for you too. They add a lot of junk that tracks who you're sharing it with.


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InvisibleBen Phartin

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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dowodenum]
    #28204089 - 02/25/23 01:02 PM (11 months, 14 days ago)

Quote:

dowodenum said:
Quote:

Ben Phartin said:
They are back in stock with prime delivery for $13.99 as of right now if anyone has been waiting on them like I was.

https://www.amazon.com/gp/product/B083HXXB34




Heh they're gone already. Cleaned up the Amazon link for you too. They add a lot of junk that tracks who you're sharing it with.





Cheers!

I still see them in stock right now



Edited by Ben Phartin (02/25/23 01:03 PM)


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Invisibledowodenum
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Ben Phartin]
    #28204105 - 02/25/23 01:08 PM (11 months, 14 days ago)

Huh, I see them unavailable in a normal tab, but available in a private tab? Might be because I'm logged in, with a Canadian account. Amazon is spooky. Pretty nice price though!


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Offlinehedgehopper
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Mr. Alien]
    #28250794 - 03/28/23 10:11 AM (10 months, 14 days ago)

What is the best way to do A2G with these containers?

I just put 7 of these to grain and the process was really not smooth... I tried to cut the agar up like tic tac toe then scrape them off into the grain but I've got a really bad feeling I am gonna end up with 7 jars of germs :lol:

Is A2G not feasible with these? I might just use them for transfers to deli cups that will probably be easier to A2G. Unless there is an easier way of getting A2G??


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Thank you everyone for your patience and advice!



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Invisibledowodenum
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: hedgehopper]
    #28250805 - 03/28/23 10:15 AM (10 months, 14 days ago)

Cut out around the colonized agar, leaving the edges in the plate. Stick your scalpel into the edge you just made, then lever it to lift up the disc. Stick the scalpel through the backside of the cut-out agar disc, and make the drop.

If you're 100% certain the dish is clean - even the sides are free of sneaky bacteria, you can smack it upside down to loosen the whole puck and do a tiger drop.

It's easier to start with fewer drops per plate. Keeps exposure time down, more eggs in more baskets.

If you shake your grain jar hard enough and use soft enough agar, it'll break up in the jar anyway.


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Offlinehedgehopper
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dowodenum]
    #28250889 - 03/28/23 11:14 AM (10 months, 14 days ago)

Quote:

dowodenum said:
Cut out around the colonized agar, leaving the edges in the plate. Stick your scalpel into the edge you just made, then lever it to lift up the disc. Stick the scalpel through the backside of the cut-out agar disc, and make the drop.

If you're 100% certain the dish is clean - even the sides are free of sneaky bacteria, you can smack it upside down to loosen the whole puck and do a tiger drop.

It's easier to start with fewer drops per plate. Keeps exposure time down, more eggs in more baskets.

If you shake your grain jar hard enough and use soft enough agar, it'll break up in the jar anyway.




Ok, I will try to just drop one puck each for my next batch later this week. I tried to smack the puck a few times to get it loose from the bottom but it must have been too thick. I also don't want to stir up too much air in my box by swinging a puck around :lol:

Thx for the tips


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Thank you everyone for your patience and advice!



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Invisibledowodenum
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: hedgehopper]
    #28251046 - 03/28/23 12:41 PM (10 months, 14 days ago)

Right, sorry. Smack is more good for FH. Though I guess you could loosen them all prior to going into the SAB.


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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: dowodenum] * 3
    #28253742 - 03/29/23 09:18 PM (10 months, 13 days ago)

Off topic but I swear I thought your profile picture was a bird until I zoomed in.

Tell me I'm not the only one...

:teabird:


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Edited by Rhizomorph (03/29/23 09:19 PM)


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InvisibleYuggoth
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Exbat]
    #28270983 - 04/10/23 11:03 AM (10 months, 1 day ago)

Quote:

Exbat said:
I just found these in-stock on Amazon: https://www.amazon.com/gp/product/B09JN6G7MX/. They look like the right ones, and seller confirmed they're PP, and a customer review states that they withstand PC use.

10 for $12.99, 20 for $22.99. Ships from Amazon.




I decided to try these containers, though I ordered the 8 oz variant. I wanted a wider agar surface so I could do streaking, plus I thought that the containers unused for agar would be useful for other things.

The clarity is quite good. Unfortunately they don't seal perfectly. If I put some water in and screw the top on, then shake the dickens out of it, I can get drops of water to leak out. Of course, I do not plan on shaking my cultures like a crying baby, but I was hoping for a perfect seal.

I will do a batch of no-pour plates soon.

LMK if I can answer any questions about these containers.



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OfflineTCan4
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Yuggoth]
    #28271955 - 04/10/23 09:27 PM (10 months, 1 day ago)

Does anyone have data on just stacking the containers on the trivet and PCing? What is the use of the tall container?


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InvisibleYuggoth
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: TCan4]
    #28280733 - 04/16/23 03:29 PM (9 months, 26 days ago)

I did a no-pour agar run with the 8 oz containers shown above, with mixed results.

I didn't have a way other than foil to enclose the stack of containers so I knew I'd have condensation, but I wanted to give it a go anyway.

I found that a stack of the containers was hard to work with... It was very wobbly. It was a little tricky wrapping the stacks in foil without them falling over.

And, despite my best efforts to close them all loosely, 2 of the 15 were closed too tightly, and imploded.

The survivors are in a warm place to see if anything grows, and hopefully to clear up the condensation. We'll see what happens. But if for no other reason that the wobbly stacking, these are no "holy grail" containers. The lack of a water-tight seal when screwed down tightly is also concerning.

I thought no-pour would be easier, but even if these containers perform perfectly I have realized that the work of prepping the plates, stacking them, PCing them, and handling them afterwards doesn't really seem like less work than PCing a bottle of agar and pouring it.



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Invisiblemyc_ousin_vinny
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: TCan4]
    #28280765 - 04/16/23 04:00 PM (9 months, 26 days ago)

Quote:

TCan4 said:
Does anyone have data on just stacking the containers on the trivet and PCing? What is the use of the tall container?




The idea is to prevent condensation. I don’t find that using the quart jars for that purpose does much.


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InvisibleRhizomorph
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Yuggoth] * 1
    #28281456 - 04/17/23 01:27 AM (9 months, 26 days ago)

Quote:

Yuggoth said:
But if for no other reason that the wobbly stacking, these are no "holy grail" containers



It's almost like the OP used a container that prevents wobbling and recommended the same...

Quote:

Yuggoth said:
The lack of a water-tight seal when screwed down tightly is also concerning



I've had 0 contams so far and every plate comes out of the PC with some water in the threads. If you're really that worried give them a shake and the water flings out.

Quote:

Yuggoth said:
despite my best efforts to close them all loosely, 2 of the 15 were closed too tightly, and imploded



Any plates that have "imploded" for me have only had a mild concave that doesn't impair the functioning. The only plates that have imploded also imploded in the first run through the PC with no issues in consecutive runs. My guess is it has to do with the fact that you bought containers that are noticeably different than the ones suggested by the OP, which have a lip/different seal.

Your issues/concerns seem premature, no offense. I'm only being blunt because you're quick to criticize issues that have easy solutions in the OP/rest of the thread and you're not following the OP's instructions properly. You get a shitty cake if you skip the step about adding baking soda :shrug:

All the best to your future plates :grin:


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Edited by Rhizomorph (04/17/23 01:36 AM)


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InvisibleYuggoth
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Re: Alien's Holy Grail No pour Agar unmodified containers. [Re: Rhizomorph]
    #28281463 - 04/17/23 01:51 AM (9 months, 26 days ago)

OP’s containers are apparently no longer availableβ€”not without caveats about bad lids anyway. So, I tried something different and reported some observations. I’m not criticizing OP, the post was very helpful. I think finding another source of quality containers would be helpful too.


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