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InvisibleKeyMaker
Urban Wizard

Registered: 01/03/22
Posts: 518
Loc: in a van down by the river
Re: Ask Quick Questions, Get Quick Answers [Re: Grogu] * 1
    #28247766 - 03/26/23 02:32 PM (9 months, 29 days ago)

Quote:

Grogu said:
Quote:

KeyMaker said:
Do you store them in the fridge or what?




Not yet, it's a new tek a friend shared, I just put all 8 in 3x4 baggies and do them about a week or so before sending, but I guess once they've colonized the agar piece they could be stored in fridge. I suggest recepient use a staralized paper clip to get them out or maybe they might just fall out if tapped a little!?

:ancientaliens:



And they come sterile in the package, so you just cut out a piece in front of flow how/in sab, and put the cap on?  Sounds like this is best suited for sharing, but smellyhobbit said it's good for using in the future?  How far into the future?


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Invisibledowodenum
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Registered: 08/05/19
Posts: 1,268
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Re: Ask Quick Questions, Get Quick Answers [Re: KeyMaker]
    #28247844 - 03/26/23 03:26 PM (9 months, 29 days ago)

I believe they're pp5 and can be sterilized. LC or distilled water slants are good for years. I heard 6 years for LC in the fridge once, could probably go even longer though.


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OfflineSmellyhobbit
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Registered: 04/01/22
Posts: 11,151
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Re: Ask Quick Questions, Get Quick Answers [Re: dowodenum] * 1
    #28247871 - 03/26/23 03:52 PM (9 months, 28 days ago)

I’m sure the upper limit for LC is unknown. Probably unguessable. So long as the conditions are good, which they should always be for our purposes, I imagine it can last longer than any of us think


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OfflineNotSheekle
OTD FOREVER
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Registered: 02/05/23
Posts: 2,055
Loc: Libertatia
Last seen: 1 month, 24 days
Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28247898 - 03/26/23 04:07 PM (9 months, 28 days ago)

Yea I guess you’re right, most of us cannot guarantee a stable temperature for prolong periods of time, if the temp sways in your house it sways in your fridge, it sways in your sample.. after that there are only so much nutrients you can provide it before theres nothing.


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OfflineSan Pedro GirlS
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Re: Ask Quick Questions, Get Quick Answers [Re: NotSheekle]
    #28247909 - 03/26/23 04:15 PM (9 months, 28 days ago)

Quote:

NotSheekle said:
Yea I guess you’re right, most of us cannot guarantee a stable temperature for prolong periods of time, if the temp sways in your house it sways in your fridge, it sways in your sample.. after that there are only so much nutrients you can provide it before theres nothing.



If your house temp changes your fridge temp, you have a shit fridge.


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OfflineNotSheekle
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Registered: 02/05/23
Posts: 2,055
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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl] * 1
    #28247925 - 03/26/23 04:22 PM (9 months, 28 days ago)

You ain’t wrong but I think it’s very common, you do really well at creating a home lab and managing your specs. I think you have farless to worry about as someone like me who’s walls have no insulation, who’s air and heat are not centralized and who has to work with only what they’ve got. Not to say you don’t find your own pitfalls and challenges, I am confident you have finding your way to where you are now, I however, have never had a house/fridge/grow that had stable temps.


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OfflineSmellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: NotSheekle]
    #28247929 - 03/26/23 04:24 PM (9 months, 28 days ago)

As I understand it LC runs out of nutrients at full colonization. After that it’s just in stasis anyway. There’s not 4+ years of nutes in a 500ml bottle. We know it can last at least that long.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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OfflineMushroomMania007


Registered: 03/15/23
Posts: 139
Last seen: 9 months, 16 days
Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28248043 - 03/26/23 05:34 PM (9 months, 28 days ago)

Is the white blobs ok ? Or more FAE



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Invisiblemyc_ousin_vinny
Keeping_It_Real
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Registered: 04/29/20
Posts: 1,415
Re: Ask Quick Questions, Get Quick Answers [Re: MushroomMania007]
    #28248074 - 03/26/23 06:04 PM (9 months, 28 days ago)

Quote:

MushroomMania007 said:
Is the white blobs ok ? Or more FAE






Those aren’t blobs really. Looks like healthy cakes to me. Stay the course.


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OfflineMushroomMania007


Registered: 03/15/23
Posts: 139
Last seen: 9 months, 16 days
Re: Ask Quick Questions, Get Quick Answers [Re: myc_ousin_vinny]
    #28248080 - 03/26/23 06:06 PM (9 months, 28 days ago)

Thank you ! I’ve been reading about Arial mycellium the one in the middle , has a few pointy things .. I’ll fan more mist less maybe.  007


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OfflineScrewup
Googles your dumb questions
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Registered: 01/27/22
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Re: Ask Quick Questions, Get Quick Answers [Re: MushroomMania007]
    #28248096 - 03/26/23 06:14 PM (9 months, 28 days ago)

Or don’t fan at all and only mist when there isn’t micro droplets on the surface of your cakes.


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Invisiblebakedbeings
orbiter of truth
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Registered: 09/01/20
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Re: Ask Quick Questions, Get Quick Answers [Re: Screwup]
    #28248100 - 03/26/23 06:17 PM (9 months, 28 days ago)

Quote:

Screwup said:
Or don’t fan at all and only mist when there isn’t micro droplets on the surface of your cakes.



:whathesaid:


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OfflineProposition 122
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Registered: 11/14/22
Posts: 375
Loc: Colorado Flag
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Re: Ask Quick Questions, Get Quick Answers [Re: NotSheekle]
    #28248176 - 03/26/23 07:05 PM (9 months, 28 days ago)

Quote:

NotSheekle said:
You ain’t wrong but I think it’s very common, you do really well at creating a home lab and managing your specs. I think you have farless to worry about as someone like me who’s walls have no insulation, who’s air and heat are not centralized and who has to work with only what they’ve got. Not to say you don’t find your own pitfalls and challenges, I am confident you have finding your way to where you are now, I however, have never had a house/fridge/grow that had stable temps.



I have had a lot of crapy friges in the various rental places I have lived, with conditions like you mention, but it never affected the frig. Even the one with the frayed seal. It just caused the frig to work more/harder. My 2-cents.
Have you noticed the the cents sign has vanished from the keyboards!


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"Learn from yesterday, live for today, hope for tomorrow. The important thing is not to stop questioning." - Albert Einstein


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OfflineMushroomMania007


Registered: 03/15/23
Posts: 139
Last seen: 9 months, 16 days
Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28248214 - 03/26/23 07:28 PM (9 months, 28 days ago)

They just look differently than my last this is my 2nd attempt



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OfflineSmellyhobbit
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Registered: 04/01/22
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Re: Ask Quick Questions, Get Quick Answers [Re: MushroomMania007]
    #28248223 - 03/26/23 07:33 PM (9 months, 28 days ago)

Looks good to me


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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Invisiblepbnj
Stranger

Registered: 03/26/23
Posts: 112
Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28248243 - 03/26/23 07:41 PM (9 months, 28 days ago)

How often do you guys see pins on your agar plates? 5%-10% of the time?

I'm streaking a bunch of 60mm plates and just waiting for pins to clone.


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OfflineGrogu
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Registered: 07/02/22
Posts: 1,712
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Re: Ask Quick Questions, Get Quick Answers [Re: KeyMaker]
    #28248247 - 03/26/23 07:43 PM (9 months, 28 days ago)

Quote:

KeyMaker said:
Quote:

Grogu said:
Quote:

KeyMaker said:
Do you store them in the fridge or what?




Not yet, it's a new tek a friend shared, I just put all 8 in 3x4 baggies and do them about a week or so before sending, but I guess once they've colonized the agar piece they could be stored in fridge. I suggest recepient use a staralized paper clip to get them out or maybe they might just fall out if tapped a little!?

:ancientaliens:



And they come sterile in the package, so you just cut out a piece in front of flow how/in sab, and put the cap on?  Sounds like this is best suited for sharing, but smellyhobbit said it's good for using in the future?  How far into the future?




Hi, They come in a bag with a hole in it, I figured they were ok to just put agar piece in and mail and have checked a couple times to see if there's contam but havne't seen it yet, knock on wood...

I'm guessing they'd last a couple months in the fridge after they colonized and I noticed they all almost are not colonzing the agar piece too quickly except a couple that are fast colonizers...

https://www.amazon.com/dp/B082WXKT8K

:deathstar:


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InvisibleTormatoMFacebookDiscordReddit
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Trusted Cultivator
Re: Ask Quick Questions, Get Quick Answers [Re: pbnj]
    #28248253 - 03/26/23 07:45 PM (9 months, 28 days ago)

Quote:

pbnj said:
How often do you guys see pins on your agar plates? 5%-10% of the time?

I'm streaking a bunch of 60mm plates and just waiting for pins to clone.




It's not a set percentage. Depends on genetics, agar, conditions, etc.


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OfflineMushroomMania007


Registered: 03/15/23
Posts: 139
Last seen: 9 months, 16 days
Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28248260 - 03/26/23 07:47 PM (9 months, 28 days ago)

Quote:

Smellyhobbit said:
Looks good to me




Ok I know , This is my 2nd time . Last time they didn’t do like this it was more smooth.

I’ve been reading about the reaching mycelium everything here is so outdated eh ..


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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: Ask Quick Questions, Get Quick Answers [Re: Grogu]
    #28248263 - 03/26/23 07:49 PM (9 months, 28 days ago)

I have about 30 cultures in distilled water cryo vials. I did test one and it is clean. Basically I bought 15ml cryo vials, filled about 3/4 of distilled water, PC them, once cool I dropped in a small colonized agar piece about the size of a grain of rice. A little myc grew off the agar and floats around in stasis. Now I have a steril culture that will last years. No need to refrigerate and takes less room than slants.


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