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dream.culture
Stranger


Registered: 08/08/17
Posts: 115
Last seen: 1 month, 7 days
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attempt on cloning 1 year old dried mushroom: Contaminations showing up
#28231019 - 03/15/23 06:19 PM (10 months, 8 days ago) |
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Hi all, I'm attempting to clone 1 year old dried mushrooms: FIrst attempt failed because of too many contaminations This time I have used Hydrogen peroxide in my agar mix (PDA) and I dunked the dried mushrooms in a peroxide solution. I don't remember the dilution ratios but I can look it up if someone is interested. The contaminations seemed to be a lot less with peroxide added but they are catching up. the mycellium is tainted black. Hoping that the mycellium outrun the contamination. Please have a look and tell me what you think.
Following pictures are of the main (mother) dish where I placed the mushroom stem after dunking it in peroxide solution.
   
Now I'm posting pics of the trasfers (2 in total from one mother dish) I did from the above as soon as I saw enough growth:
     
some thoughts will be appriciated. I'm planning on making more transfers from the mother dish and its transfers from the trailing edges that don't look as contaminated.
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ElonMuskrat
I Throw Bags Out For A Living


Registered: 06/30/09
Posts: 167
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Re: attempt on cloning 1 year old dried mushroom: Contaminations showing up [Re: dream.culture]
#28233359 - 03/17/23 08:10 AM (10 months, 6 days ago) |
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wow, super sick.
Is it cloned or germinated spores?
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Giermmo
You fuckin' druggo



Registered: 01/01/22
Posts: 360
Last seen: 10 hours, 55 minutes
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Re: attempt on cloning 1 year old dried mushroom: Contaminations showing up [Re: ElonMuskrat] 1
#28233420 - 03/17/23 08:53 AM (10 months, 6 days ago) |
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IMO you're growing some kind of mold, not cube mycelium. Get inner tissue not the whole stem lol. If you used too high heat to dry them it won't probably work anyway.
-------------------- Don't worry mate, what you're gonna feel is a slight burning in your heart, your lungs, your brain, your kidneys, your liver, bottom of your feet, your eyeballs, then you start bleeding from your nose and ears, then you start tripping out and seeing everything in the form of an anime cartoon which is really fucking cool.
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dream.culture
Stranger


Registered: 08/08/17
Posts: 115
Last seen: 1 month, 7 days
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Re: attempt on cloning 1 year old dried mushroom: Contaminations showing up [Re: ElonMuskrat]
#28234130 - 03/17/23 06:41 PM (10 months, 6 days ago) |
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I believe it isn't the spores germinating because the growth was much faster. Also they hydrogen peroxide would have made it hard for spores to germinate if not impossible.
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dream.culture
Stranger


Registered: 08/08/17
Posts: 115
Last seen: 1 month, 7 days
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Re: attempt on cloning 1 year old dried mushroom: Contaminations showing up [Re: Giermmo]
#28234134 - 03/17/23 06:46 PM (10 months, 6 days ago) |
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Yeah I'm keeping my fingers crossed. I have a feeling there is definitely some cube mycelium in there along with tons of other shit. So not sure if I will be able to clean anything out or what ill be cleaning but i'll try a few more transfers. I also took tissue from the insides on another plate, basically had plates from several different parts. The fruit was dried at the lowest heat setting last year so that won't be a problem. Anyway ill keep posting updates. Not sure if I posted this in the right section.
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