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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
ampicillin, cefazolin ?
    #2822513 - 06/24/04 12:10 AM (13 years, 2 months ago)



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Offlinetubervegan
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Registered: 07/02/04
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Re: ampicillin, cefazolin ? [Re: matts]
    #2856305 - 07/04/04 02:28 PM (13 years, 2 months ago)

well the mushrooms should be fine, I don't know about you or whoever is eating the mushrooms though...

I would suggest using the lowest concentrations possible to prevent bacterial contamination, but on the other hand I have feeling you are using these as an extra safety net, which is not a good idea. Don't use anti-biotic unless you need to, because unless you are trying to fight a specific re-occuring problem, you don't really have an idea of how much you need, or if you need it at all. don't use antibiotics blindly.


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Invisiblemicro
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Re: ampicillin, cefazolin ? [Re: matts]
    #2856507 - 07/04/04 04:20 PM (13 years, 2 months ago)

Ampicillin is used a lot in labs (about 100ug/ml) -- it's a broad-spectrum penicillin and works very well. I've never heard of cefazolin being used in agar -- it's a cephalosporin that's active against most aerobes, but only moderately active against anaerobes (but you shouldn't be growing anaerobes, anyway, so it shouldn't matter.)

I would recommend the ampicillin -- time tested.

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Micro


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
Re: ampicillin, cefazolin ? [Re: tubervegan]
    #2856826 - 07/04/04 07:18 PM (13 years, 2 months ago)



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OfflinePsilygirl
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Re: ampicillin, cefazolin ? [Re: matts]
    #2862120 - 07/06/04 06:54 PM (13 years, 2 months ago)

just so you know... ampicillin is heat-sensitive, and must be kept frozen until just before adding it to the agar--after PCing and cooled to about 55C. i have only worked with ampicillin at my job, never with cultivating.

gentamycin sulfate can be added prior to pressure cooking, and i use this antibiotic for my cultivation


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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