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North Spore Shop: Cultivation Supplies

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OfflineThugnar
Newbie in the stream
I'm a teapot


Registered: 01/12/23
Posts: 328
Loc: Next to Mordor
Last seen: 10 months, 10 days
Tyndallization and polystyrene petri dishes.
    #28224716 - 03/11/23 06:12 AM (10 months, 12 days ago)

Hello. Im not really sure if this belongs here but by brother asked to give it a go because he cant. Or could but doesnt want to make another account cause he got banned as my "puppet". =D
What ever the case.. Here i ask for assistance.

My brother was succesful in using a adjusted tyndallisation method to salvage some polystyrene(single use) control dishes and contaminated test dishes with only 2-4 unconfirmed contaminant colonies. Also some dishes with ~20 unconfirmed contaminant colonies. Almost all of them were under 2mm in diameter and white in color. Pre-stages i quess?
The method has two stages and the first one is done in +73c max temperature (beginning as pasteurization) .
Second one in +76-80°c depending on the quality of the dishes and heating/temp measuring equipment.
As a side test he also heated to pasteurizing temps some clean dishes that were knocked over and got a bit messed up.
They settled again nicely and are completely usable.
He wrote the test notes really well but its in finnish. I think you get the idea without the notes also but if someone asks, i can consider translating them when i have the time.
The assistance im asking for is modern study papers about the subject or something similar. If shroomery could provide something that he cant find himself then his future experiements to make no-pour polysterene dishes could be much more easier or even have better multifunctioning results. This one contains the information he already has. Thank you for your time.
(link downloads a pdf from science direct)


https://www.google.fi/url?sa=t&source=web&rct=j&url=https://www.sciencedirect.com/science/article/am/pii/S0308814621016988&ved=2ahUKEwjEy9-G9dP9AhWDuosKHfpWCkgQFnoECAoQAQ&usg=AOvVaw0IpWYi-kAwEY9u3n7lPxDk


--------------------
Seems like 99% of people in shroomery mushcult use the Bucket Tek. I do not and for saying this they call me arrogant cause i dont have pictures of my own mushrooms here. The mycoforum of my country just got their proof of shroomerys hostility. I do like it here though becaus of the agar. The archives are valuable.


Edited by Thugnar (03/11/23 06:15 AM)


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North Spore Shop: Cultivation Supplies


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